Dr. Kim Jae-Yean
Professor/ Division of Applied Life Science (BK21 Four program)
Division of Life Science
Gyeongsang National University (GNU)
Founder & CEO, Nulla Bio Inc.
kimjy@gnu.ac.kr
Education
l Ph.D. Plant Molecular & Cell Biology, Univ. Paris XI. France (1998)
l M.S. Biotechnology KAIST, Korea (1992),
l B.S. Agricultural Chemistry, Seoul National University, Korea (1990)
Major appointments
l CEO, Nulla Bio Inc (2022-present)
l Professor, Division of Applied Life Sciences, Gyeongsang National University (2003-present)
l The First President, Korean Society of Plant Breeding Innovation(2019-2022)
l Director, Outstanding Creative Korea(CK-1) Program for Life Science Education, GNU (2014-2019)
l Director, WCU Plant Cell Communication (2008-2013)
l Post-Doc, Cold Spring Harbor Laboratory, USA (2000-2003)
l Post-Doc, Univ. Warwick, UK (1998-2000)
Major research interests
l Development of genome editing technology in plants
l Development of genome engineered crops using CRISPR-Cas9/Cpf1
l Plant synthetic biology for therapeutics and high-value biomaterials
Selected Publications
Recent 6yr publications (from 144 publications)
l Tien Van Vu, Ngan Thi Nguyen, Jihae Kim, Young Jong Song, Thu Hoai Nguyen, Jae-Yean Kim. Optimized Dicot Prime Editing Enables Heritable Desired Edits in Tomato and Arabidopsis. Nature Plants 2024, doi.org/10.1038/s41477-024-01786-w
l Tien Van Vu, Ngan Thi Nguyen, Jihae Kim, Minh Huy Vu, Young Jong Song, Mil Thi Tran, Yeon Woo Sung, Jae-Yean Kim. Enhancing CRISPR-Cas-based gene targeting in tomato using a dominant-negative ku80 Horticulture Research 2024. In press
l Jin-hee Jeong, Eun-young Jeon, Min Ki Hwang, Young Jong Song, and Jae-Yean Kim. Development of super-infective ternary vector systems for enhancing the agrobacterium-mediated plant transformation and genome editing efficiency Horticulture Research 2024, doi:10.1093/hr/uhae187
l Rahul Mahadev Shelake, Sopan Ganpatrao Wagh, Akshay MILIND Patil, Jan Cerveny, Rajesh Ramdas Waghunde, Jae-Yean Kim. Heat Stress and Plant-Biotic Interactions: Advances and Perspectives. Plants 2024. https://doi.org/10.3390/plants13152022
l Ritesh Kumar, Arya Bagus Boedi Iswanto, Dhinesh Kumar, Wu Shuwei, Kyujin Oh, Geon Hui Son, Eun-Seok Oh, Minh Huy Vu, Jinsu Lee, Keun Woo Lee, Man-Ho Oh, Chian Kwon, Woo Sik Chung, Jae-Yean Kim* and Sang Hee Kim*. C-TYPE LECTIN RECEPTOR-LIKE KINASE 1 and ACTIN DEPOLYMERIZING FACTOR 3 negatively regulate plasmodesmata callose. Plant Cell Environment. doi.org/10.1111/pce.14957
l Arya Bagus Boedi Iswanto, Minh Huy Vu, Jong Cheol Shon, Ritesh Kumar, Shuwei Wu, Hobin Kang, Da-Ran Kim, Geon Hui Son, Woe Yoen Kim, Youn-Sig Kwak, Kwang Hyeon Liu, Sang Hee Kim and Jae-Yean Kim. α1-COP modulates plasmodesmata function through sphingolipid enzymes regulation. Journal Integrative Plant Biology, 2024. doi: 10.1111/jipb.13711
l Rahul Mahadev Shelake1, Dibyajyoti Pramanik, Jae-Yean Kim. CRISPR base editor-based targeted random mutagenesis (BE-TRM) toolbox for directed evolution. BMB Reports 2024. 10.5483/BMBRep.2023-0086.
l Cam Chau Nguyen, Rahul Mahadev Shelake, Tien Van Vu, Hai Van Tong, Nhan Thi Nguyen, Xuan Canh Nguyen, Vo-Anh-Khoa Do, Hai Thanh Nguyen, Jae-Yean Kim. Characterization of yield and fruit quality parameters of Vietnamese elite tomato lines generated through phenotypic selection and conventional breeding methods. Euphytica 2024. 10.21203/rs.3.rs-2626574/v1.
l Tien V. Vu, Ngan Thi Nguyen, Jihae Kim , Jong Chan Hong and Jae-Yean Kim. Prime Editing: Mechanism Insight and Recent Applications in Plants. Plant Biotechnology Journal 2024. https://doi.org/10.1111/pbi.14188.
l Cam Chau Nguyen, Tien Van Vu, Rahul Mahadev Shelake, Nhan Thi Nguyen, Tran Dang Khanh, Jae-Yean Kim. Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system. Molecular Breeding 2024. https://doi.org/10.1101/2023.04.27.538515.
l Swati Das, Moonhyuk Kwon and Jae-Yean Kim. Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants. Frontiers in Plant Science 2024. 10.3389/fpls.2024.1279738.
l Jin-hee Jeong, Eun-young Jeon, Young Jong Song, Min Ki Hwang, Yeongji Gwak and Jae-Yean Kim. Impact of CRISPR/Cas9-induced mutations in nicotine biosynthesis core genes A622 and BBL on tobacco: Reduction in nicotine content and developmental abnormalities. Current Plant Biology 2024, 10.1016/j.cpb.2024.100343
l Rahul Mahadev Shelake, Jae-Yean Kim. Cas9 variants expand the targeting scope of base editing systems in bacteria. Nucleus 2024. https://doi.org/10.1007/s13237-024-00473-7.
(2023)
Rajesh Ramdas Waghunde, Mrugesh Dhirajlal Khunt, Rahul Mahadev Shelake, Neelkanth Hiremani, Vijay Aadhar Patil, Jae-Yean Kim. Chapter 2 - Endophytes: a potential bioagent for plant disease management. Academic press, In Microbial Endophytes and Plant Growth 2023. 10.1016/B978-0-323-90620-3.00013-1
l Minh Huy Vu, Tae Kyung Hyun, Sungwha Bahk, Yeonhwa Jo, Ritesh Kumar, Dhinesh Kumar, Arya Bagus Boedi Iswanto, Rahul Mahadev Shelake, Woo Sik Chung, Jae-Yean Kim. ROS-mediated plasmodesmal regulation requires a network of an Arabidopsis receptor-like kinase, calmodulin-like proteins, and callose synthases . Frontiers in Plant Science 2023. 10.3389/fpls.2022.1107224.
l Rahul Mahadev Shelake, Dibyajyoti Pramanik, Rajesh Ramdas Waghunde, Jae-Yean Kim. Plant Mutagenesis Tools for Precision Breeding: Conventional CRISPR/Cas9 Tools and Beyond. Mutation Breeding for Sustainable Food Production and Climate Resilience 2023. 10.1007/978-981-16-9720-3_10.
l Mil Thi Tran, Geon Hui Son, Young Jong Song, Ngan Thi Nguyen, Seonyeong Park, Thanh Vu Thach, Jihae Kim, Yeon Woo Sung, Swati Das, Dibyajyoti Pramanik, Jinsu Lee, Ki-Ho Son, Sang Hee Kim, Tien Van Vu, Jae-Yean Kim. CRISPR-Cas9-based precise engineering of SlHyPRP1 protein towards multi-stress tolerance in tomato. Frontiers in Plant Science 2023. doi.org/10.3389/fpls.2023.1186932.
l Bich Ngoc Vu, Tien Van Vu, Jae Yong Yoo , Ngan Thi Nguyen, Ki Seong Ko, Jae-Yean Kim* and Kyun Oh Lee*. CRISPR-Cas-mediated unfolded protein response control for enhancing plant stress resistance. Frontiers in Plant Science. 10.3389/fpls.2023.1271368.
l Rahul Mahadev Shelake, Amol Maruti Jadhav, Pritam Bhagwan Bhosale, and Jae-Yean Kim. Unlocking secrets of nature's chemists: Potential of CRISPR/Cas-based tools in plant metabolic engineering for customized nutraceutical and medicinal profiles. Plant Physiology and Biochemistry 2023. https://doi.org/10.1016/j.plaphy.2023.108070.
l Ganesh Chandrakant Nikalje, Ashish Kumar Srivastava, Rahul Mahadev Shelake, Ulhas Sopanrao Kadam, Jong Chan Hong, Jae-Yean Kim, T D Nikam, Penna Suprasanna. Profiling of conserved orthologs and miRNAs for understanding their role in salt tolerance mechanism of Sesuvium portulacastrum L. Molecular Biology Reports 2023. 10.1007/s11033-023-08892-6.
l Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae-Yean Kim. Improved dual base editor systems for simultaneous conversion of adenine and cytosine in bacteria. mBio 2023.01 https://doi.org/10.1128/mbio.02296-22
(2022)
l Rahul Mahadev Shelakea, Ulhas Sopanrao Kadama, Ritesh Kumarb, Dibyajyoti Pramanika, Anil Kumar Singhc, Jae-Yean Kim. Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, challenges, and perspectives. Plant Communication 2022.07 https://doi.org/10.1016/j.xplc.2022.100417
l Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae-Yean Kim. "In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in Escherichia coli (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their Components. International Journal of Molecular Sciences. 2022.01. https://doi.org/0.3390/ijms23031145
l Tien Van Vu, Swati Das, Goetz Hensel, Jae-Yean Kim. Gene editing and beyond: what would it mean for the future of our plant breeding? Planta 2022.05. https://doi.org/10.1007/s00425-022-03906-2
l Tien Van Vu, Swati Das, Cam Chau Nguyen, Jihae Kim, Jae-Yean Kim. Single-strand annealing: molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing. Biotechnology Journal 2022.07. https://doi.org/10.1002/biot.202100413
l Arya Bagus Boedi Iswanto, Minh Huy Vu, Sharon Pike, Jihyun Lee, Hobin Kang, Geon Hui Son, Jae-Yean Kim and Sang Hee Kim. Pathogen effectors: What do they do at plasmodesmata? Molecular Plant Pathology 2022. https://doi.org/10.1111/mpp.13142
l Tien Van Vu, Ngan Thi Nguyen, Jihae Kim, Swati Das, Jinsu Lee, Jae-Yean Kim. The obstacles and potential solution clues of prime editing applications in tomato. Biodesign Research 2022. 10.34133/bdr.0001
(2021)
l Mil Thi Tran, Duong Thi Hai Doan, Jihae Kim, Yeong Jong Song, Yeon Woo Sung, Swati Das, Geon Hui Son, Sang Hee Kim, Tien Van Vu, Jae-Yean Kim. CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato. Plant Cell Report 2020.10/2021. https://doi.org/10.1007/s00299-020-02622-z
l Tien Van Vu, Duong Thi Hai Doan, Jihae Kim, Yeon Woo Sung, Mil Thi Tran, Yeong Jong Song, Swati Das, Jae-Yean Kim. CRISPR/Cas-based precision genome editing via microhomology-mediated end joining (MMEJ). Plant Biotechnology Journal 2021.02. https://doi.org/10.1111/pbi.13490
l Dibyajyoti Pramanik, Rahul Mahadev Shelake, Mi Jung Kim and Jae-Yean Kim. CRISPR-mediated engineering across the central dogma in plant biology for basic research and crop improvement. Molecular Plant 2021.01. https://doi.org/10.1016/j.molp.2020.11.002
l Dibyajyoti Pramanik, Rahul Mahadev Shelake, Jiyeon Park, Mi Jung Kim, Indeok Hwang, Younghoon Park, and Jae-Yean Kim. CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew. International Journal of Molecular Science. 2021.02. https://doi.org/10.3390/ijms22041878
l Arya Bagus Boedi Iswanto, Rahul Mahadev Shelake, Minh Huy Vu, Jae-Yean Kim and Sang Hee Kim. Genome Editing for Plasmodesmal Biology. Frontiers in Plant Science, section Plant Cell Biology. 2021.06. https://doi.org/10.3389/fpls.2021.679140
l Jin Hwa Park, Halim Park, Yejin Lee, Dong U Woo, Ho Hwi Jeon, Yeon Woo Sung, Sangrea Shim, Sang Hee Kim, Kyun Oh Lee, Jae-Yean Kim, Chang-Kug Kim, Hwan Su Yoon, Yang Jae Kang. The genome of the world's smallest flowering plant, Wolffia australiana, disclosing its unique physiology and the most extreme morphological evolution. Communication Biology 2021.06. https://doi.org/10.1038/s42003-021-02422-5
l Kumar Nishant Chourasia, Milan Kumar Lal, Rahul Kumar Tiwari, Devanshu Dev, Hemant Balasaheb Kadile, Virupaksh U. Patil, Amarjeet Kumar, Girimalla Vanishree, Dharmendra Kumar, Vinay Bhardwaj, Jitendra Kumar Meena, Vikas Mangal, Rahul Mahadev Shelake, Jae-Yean Kim, Dibyajyoti Pramanik. Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses. Life 2021.06. https://doi.org/10.3390/life11060545
l "Geon Hui Son, Jiyun Moon, Rahul Mahadev Shelake, Uyen Thi Vuong, Robert A. Ingle, Walter Gassmann, Jae-Yean Kim and Sang Hee Kim. Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1. International Journal of Molecular Sciences. 2021. https://doi.org/10.3390/ijms22126427
l Tien Van Vu, Duong Thi Hai Doan, Mil Thi Tran, Yeon Woo Sung, Young Jong Song, Jae-Yean Kim. Improvement of the LbCas12a-crRNA system for efficient gene targeting in tomato. Frontiers in Plant Science. 2021.07. https://doi.org/10.21203/rs.3.rs-301508/v2
(2020)
l Vu TV, Swati Das1, Tran MT, Hong JC, Kim JY 2020. Precision Genome Engineering for the Breeding of Tomatoes: Recent Progress and Future Perspectives. Front in Genome Editing. Doi:10.3389/fgeed.2020.612137.
l Iswanto ABB, Shon JC,.., Liu GH, Kim JY 2020. Sphingolipids Involve the Modulation of the Secretion Machinery of a Subset of Plasmodesmata Proteins and a Change in the Plasmodesmata Callose Level. Plant Physiology. 184(1)407-420.
l Vu MH, Iswanto ABB, Lee J, Kim JY 2020. The role of plasmodesmata associated-receptor in plant development and environmental response. Plants. 9(2):216
l Vu VT, ..,Kim JY et al. 2020. Highly efficient homology-directed repair using CRISPR/Cpf1-geminiviral replicon in tomato. Plant Biotechnology Journal. 18(10) 2133-2143
l Kim SH, Bahk S, An J, Hussain S, Nguyen NT, Do HL, Kim JY, Chung WS 2020. A gain-of-function mutant of IAA15 inhibits lateral root development by transcriptional repression of LBD genes in Arabidopsis. Frontiers in Plant Science. 11, 1239
l Rajesh Ramdas Waghunde, Rahul Mahadev Shelake, Dibyajyoti Pramanik, Harish Sutar, Puja Pandey, Jae-Yean Kim. Application of cereal and vegetable endophytes in plant health management. In Microbiomes and plant health: Panoply and their applications 10.1016/B978-0-12-819715-8.00002-1.
l Pankaj Prakash Verma, Rahul Mahadev Shelake, Parul Sharma, Jae-Yean Kim, Suvendu Das, Mohinder Kaur. Microbes in crop production: Formulation and Application. Microcosm: Microbial Diversity, Interventions and Scope. doi.org/10.1007/978-981-15-4099-8_3
(2019)
l 김재연, 손건희 유전자가위 기술을 활용한 정밀육종 신품종 개발의 혜택. Biosafety 2019
l Rahul Mahadev Shelake, Rajesh Ramdas Waghunde, Pankaj Prakash Verma, Chandrakant Singh, Jae-Yean Kim. Carbon Sequestration for Soil Fertility Management: Microbiological Perspective. Soil Fertility Management for Sustainable Development. doi.org/10.1007/978-981-13-5904-0_3
l Amir Zeb, Donghwan Kim, Sayed Ibrar Alam, Minky Son, Raj Kumar, Shailima Rampogu, Saravanan Parameswaran, Rahul Mahadev Shelake, Rabia Mukhtar Rana, Shraddha Parate, Jae-Yean Kim, Keun Woo Lee. Computational Simulations Identify Pyrrolidine-2,3-dione Derivatives as Novel Inhibitors of Cdk5/p25 Complex to Attenuate Alzheimer’s Pathology. Journal of Clinical Medicine. doi.org/10.3390/jcm8050746
l Rahul Mahadev Shelake, Rajesh Ramdas Waghunde, Jae-Yean Kim. Plant–Microbe–Metal (PMM) Interactions and Strategies for Remediating Metal Ions. Plant-Metal Interactions (Springer Nature). doi.org/10.1007/978-3-030-20732-8_12.
l Rahul Mahadev Shelake, Dibyajyoti Pramanik and Jae-Yean Kim. Exploration of Plant-microbe Interactions for Sustainable Agriculture in CRISPR Era. Microorganisms 2019. doi:10.3390/microorganisms7080269
l Dongwon Baek, Min Chul Kim, Dhinesh Kumar, Bokyung Park, Mi Sun Cheong, Wonkyun Choi, Hyeong Cheol Park, Hyun Jin Chun, Hee Jin Park, Sang Yeol Lee, Ray A. Bressan, Jae-Yean Kim, Dae-Jin Yun. AtPR5K2, a PR5-Like Receptor Kinase, Modulates Plant Responses to Drought Stress by Phosphorylating Protein Phosphatase 2Cs. Frontiers in Plant Science 2019. doi.org/10.3389/fpls.2019.01146
l Rahul Mahadev Shelake, Dibyajyoti Pramanik and Jae-Yean Kim. Evolution of plant mutagenesis tools: A shifting paradigm from random to targeted genome editing. Plant Biotechnology Reports 2019. doi.org/10.1007/s11816-019-00562-z.
l Jeong-II Kim, Jae-Yean Kim. New era of precision plant breeding using genome editing. Plant Biotechnology Reports 2019. doi.org/10.1007/s11816-019-00562-z.
l Tien Van Vu, Yeon Woo Sung, Jihae Kim, Duong Thi Hai Doan, Mil Thi Tran, Jae-Yean Kim. Challenges and perspectives in homology-directed Gene targeting in monocot plants. Rice 2019. doi.org/10.1007/s11816-019-00581-w.
l Pankaj Prakash Verma, Rahul Mahadev Shelake, Suvendu Das, Parul Sharma, Jae-Yean Kim. Plant growth-promoting rhizobacteria (PGPR) and fungi (PGPF): Potential biological control agents of diseases and pests. Microbial interventions in agriculture and environment, (Springer Nature). https://doi.org/10.1007/978-981-13-8391-5_11.