Publications

Journal publications:

2025

103 Endo, K., Akiyama, H., Kano, K., Mishiro-Sato, E., Karashima, T., Kajiwara, Y., Takashita, H., Shimizu, K., Honda, H.: Identification of barley-derived peptides with angiotensin converting enzyme inhibitory activity, International Journal of Food Engineering, Accepted

2024

102 Nagai, A., Kaneda, Y., Izumo, T., Nakao, Y., Honda, H., Shimizu, K.: Quercetin induces a slow myofiber phenotype in engineered human skeletal muscle tissues, The FASEB Journal, 38: e70009 (2024)

Selected as a Cover Image

101 Fujii, I., Kinoshita, R., Akiyama, H., Nakamura, A., Iwamori, k., Fukada, S., Honda, H., Shimizu, K.: Discovery of Fibroblast Growth Factor 2-Derived Peptides for Enhancing Mice Skeletal Muscle Satellite Cell Proliferation, Biotechnology Journal, Accepted 

Review in Japanese 1 永井研迅、本多裕之、清水一憲：三次元ヒト骨格筋組織の筋力を指標にしたL-アンセリンの効能評価、Functional Food Research、Accepted 

100 Ding, R., Xi, Y., Ito, A., Shimizu, K., Nagamori, E., Fujita, H., Kawamoto, T., Horie, M.: BMP signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12, Journal of Bioscience and Bioengineering, Accepted

99 Iriyama, M., Hagawa, H., Shimizu, S., Akiyama, H., Shimizu, K., Honda, H.: Separation and enrichment of multiple bile acid micelle-disrupting peptides by adsorption/desorption process with heat-treated porous silica gels, Biochemical Engineering Journal, Accepted

2023

98 Murata, A., Akiyama, H., Honda, H., Shimizu, K.: Electrical pulse stimulation-induced tetanic artificial exercise increases the secretion of extracellular vesicles from C2C12 myotubes,  Biochemical and Biophysical Research Communications, 672: 177-184 (2023)

97 Nagai, A., Ida, M., Izumo, T., Nakai, M., Honda, H., Shimizu, K.: L-Anserine increases muscle differentiation and muscle contractility in human skeletal muscle cells, Journal of Agricultural and Food Chemistry, 71: 8952-8958 (2023)

Selected as a Supplementary Cover Image

96 Rashid, I. M., Ito, T., Shimojo, D., Arimoto, K., Onodera, K., Okada, R., Nagashima, T., Yamamoto, K., Khatun, Z., Okano, H., Sakurai, H., Shimizu, K., Doyu, M., Okada, Y.: Simple and efficient differentiation of human iPSCs into contractible skeletal muscle for muscular disease modeling, Scientific Reports, 13: 8146 (2023)

2022

95 Shimizu, K., Kassai, H., Kamei, Y., Nagashima, T., Yamamoto, K., Maekawa, T., Akiyama, H., Honda, H.: Alignment of cultured skeletal muscle cells facilitates acetylcholine receptor clustering and neuromuscular junction formation with co-cultured human iPSC-derived motor neurons, Cells, 11(23): 3760 (2022)

94 Mizukami, Y., Yamaguchi, T., Shiono, M., Takahashi, Y., Shimizu, K., Konishi, S., Takakura, Y., Nishikawa, M.: Drug-preloadable methacrylated gelatin microspheres fabricated using an aqueous two-phase system, European Polymer Journal, 181: 111671 (2022)

93 Tsujimura, M., Kusamori, K., Takamura, K., Ito, T., Kaya, T., Shimizu, K., Konishi, K., Nishikawa, M.: Quality evaluation of cell spheroids for transplantation by monitoring oxygen consumption using an on-chip electrochemical device , Biotechnology Reports, Accepted 

J-2 水谷嶺太、森陽子、小川翔大、田添佳歩、秋山裕和、清水一憲、本多裕之：蛍光標識ペプチドアレイを用いたプロテアーゼ切断検出と機械学習を用いた切断部位予測、生物工学会誌、Accepted

92 Kurotani, K.,Kawakatsu, Y.,  Kikkawa, M., Tabata, R., Kurihara, D., Honda, H., Shimizu, K., Notaguchi, M.: Analysis of Plasmodesmata Permeability using Cultured Tobacco BY-2 Cells Entrapped in Microfluidic Chips, Journal of Plant Research, Accepted 

91 Yamamoto, K., Ohsumi, S., Nagashima, T., Akiyama, H., Honda, H., Shimizu, K.: Screening of anti-atrophic peptides by using photo-cleavable peptide array and contractile 96-well scale human skeletal muscle atrophy models, Biotechnology and Bioengineering, Accepted 

90 Shimizu, K., Kawakatsu, Y., Kurotani, K., Kikkawa, M., Tabata, R., Kurihara, D., Honda, H., Notaguchi, M.: Development of Microfluidic Chip for Entrapping Tobacco BY-2 Cells, PLOS ONE, Accepted

89 Hagawa, H., Imai, K., Gao, Z., Taniguchi, M., Shimizu, K., Honda, H.:  Selective concentration of antimicrobial peptides to heat-treated porous silica gel using adsorption / desorption, Journal of Bioscience and Bioengineering, 133(2): 161-167 (2022)

88 Shimazawa, Y., Kusamori, K., Tsujimura, M., Shimomura, A., Takasaki, R., Takayama, Y., Shimizu, K., Konishi, S., Nishikawa, M.: Intravenous injection of mesenchymal stem cell spheroids for efficient pulmonary delivery and prolonged in vivo survival, Biotechnology Journal, 17(1):2100137 (2022) 

Selected as a back cover image

2021

87 Kinoshita, R., Kozaki, I., Shimizu, K., Shibata, T., Ochiai, A., Honda, H.: Agonist/antagonist activity of oxytocin variants obtained from free cyclic peptide libraries generated via amino acid substitution, ACS Omega, 6(46): 31244-31252 (2021)

86 Imai, K., Takeuchi, Y., Shimizu, K., Honda, H.: In silico screening of a bile acid micelle disruption peptide for oral consumption from edible peptide database, Foods, 10(10): 2496 (2021)

85 Mizukami, Y., Takahashi, Y., Shimizu, K., Konishi, S., Takakura, Y., Nishikawa, M.: Calcium peroxide-containing polydimethylsiloxane-based microwells for inhibiting cell death in spheroids through improved oxygen supply, Biological and Pharmaceutical Bulletin, 44(10): 1458-1464 (2021)

84 Imai, K., Shimizu, K., Honda, H.: Machine learning screening of bile acid-binding peptides in a peptide database derived from food proteins, Scientific Reports, 11: 16123 (2021)

83Kirihara, Y., Miyata, H., Peng, Z., Shimba, K., Miyamoto, Y., Shimizu, K., Honda, H., Yagi, T.: Effect of Magnetic Nanoparticle Internalization on Cell Density in Skeletal Muscle Tissue, IEEJ Transactions on Electrical and Electronic Engineering, 141(7):795-801 (2021)

82Fujita, H., Horie, M., Shimizu, K., Nagamori, E.: Microarray profiling of gene expression in C2C12 myotubes trained by electric pulse stimulation,  Journal of Bioscience and Bioengineering, Accepted

81 Ishihara, M., Kikkawa, M., Shimizu, K., Suzuki, H.., Honda, H.: Simple Stain-free Screening Method for Pectinolysis Microorganisms under Alkalophilic Condition, Biotechnology Letters, 43: 1905-1911 (2021)

80 Yamamoto, K., Yamaoka, N., Imaizumi, Y., Nagashima, T., Furutani, T., Ito, T., Okada, Y., Honda, H., Shimizu, K.: Development of a Human Neuromuscular Tissue-on-a-Chip Model on a 24-Well-Plate-Format Compartmentalized Microfluidic Device, Lab on a Chip, 21: 1897-1907 (2021)

Selected as a back cover image

79 Dau, VT., Vu, TH., Tran, CD., Nguyen, TV., Nguyen, TK., Dinh, T., Phan, HP., Shimizu, K., Nguyen, NT., Dao, DV.: Electrospray propelled by ionic wind in a bipolar system for direct delivery of charge reduced nanoparticles, Applied Physics Express, 14: 055001

78 Yoshioka, K., Yamashita, H., Shimizu, K., Shimomura, S., Shibata, T., Miyazaki, J., Honda, H.: Screening of a novel free fatty acid receptor 1 (FFAR1) agonist peptide by phage display and machine learning based-amino acid substitution, Biochemical and Biophysical Research Communications, 550(23): 177-183 (2021)

Original Article in Japanese 1 (J-1) 

吉岡慶太朗、山下春来、藤谷将也、加藤竜司、清水一憲、本多裕之：分子構造相同性解析を用いた脂肪酸受容体刺激ペプチドの探索、化学工学論文集、Accepted

77 Yoshioka, K., Ito, A., Arifuzzaman, Md., Yoshigai, T., Fan, F., Sato, K., Shimizu, K., Kawabe, Y., Kamihira, M.: Miniaturized skeletal muscle tissue fabrication for measuring contractile activity, Journal of Bioscience and Bioengineering, 131(4): 434-441 (2021)

76 Kozaki, I., Suzuki, T., You, S-C., Shimizu, K., Honda, H.: Increasing the activity of cell adherent cyclic NGR peptides by optimizing the peptide length and amino acid character, Journal of Peptide Science, 27: e3287 (2021)

2020

75 Nagashima, T., Hadiwidjaja, S., Ohsumi, S., Murata, A., Hisada, T., Kato, R., Okada, Y., Honda, H., Shimizu, K.: In vitro model of human skeletal muscle tissues with contractility fabricated by immortalized human myogenic cells,  Advanced Biosystems, 4: 2000121 (2020)

74 Yamashita, H., Fujitani, M., Shimizu, K., Kanie, K., Kato, R., Honda, H.: Machine learning-based amino-acid substitution of short peptides: Acquisition of peptides with enhanced inhibitory activities against α-amylase and α-glucosidase,  ACS Biometerials Science & Engineering, 6: 6117-6125 (2020)

73 Ito, M., Shimizu, K., Honda, H.: Bile acid micelle disruption activity of short-chain peptides from tryptic hydrolysate of edible proteins, Journal of Bioscience and Bioengineering, 130(5): 514-519 (2020)

72 Mizukami, Y., Moriya, A., Takahashi, Y., Shimizu, K., Konishi, S., Takakura, Y., Nishikawa, M.: Incorporation of gelatin microspheres into HepG2 human hepatocyte spheroids for functional improvement through improved oxygen supply to spheroid core, Biological Pharmaceutical Bulletin, 43(8): 1220-1225 (2020)

71 Ding, R., Horie, M., Nagasaka, S., Ohsumi, S., Shimizu, K., Honda, H., Nagamori, E., Fujita, H., Kawamoto, T.: The effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue, Journal of Bioscience and Bioengineering, 130(1): 98-105 (2020)

70 Taniguchi, Y.,  Oyama, N., Fumoto, S., Kinoshita, H.,Yamashita, F., Shimizu, K., Hashida, M., Kawakami, S.: Tissue suction-mediated gene transfer to the beating heart in mice, PLoS ONE, 15(2): e0228203 (2020)

69 Shimizu, K., Ohsumi, S., Kishida, T., Mazda, O., Honda, H.: Fabrication of Contractile Skeletal Muscle Tissues Using Directly Converted Myoblasts from Human Fibroblasts, Journal of Bioscience and Bioengineering,129(5): 632-637 (2020)

68 Kozaki, I., Shimizu, K., Honda, H.: Disulfide linked hetero dimeric peptide arrays for screening functional peptides inside cells, Journal of Bioscience and Bioengineering, 129(5): 613-618 (2020)

2019

67 Kurimoto, M., Shimizu, K., Ochi, H., Abe, F., Honda, H.: Protease hydrolysis assay system using photo-cleavable peptide array and mass spectrometer (Pep-MS assay), Journal of Bioscience and Bioengineering, 128(2): 156-161 (2019)

66 Imai, K., Shimizu, K., Honda, H.: Predictive selection and evaluation of appropriate functional peptides for intestinal delivery with a porous silica gel, Journal of Bioscience and Bioengineering, 128(1): 44-49 (2019)

65 Yamaoka, N., Shimizu, K., Imaizumi, Y., Ito, T., Okada, Y., Honda, H.: Open-Chamber Co-Culture Microdevices for Single-Cell Analysis of Skeletal Muscle Myotubes and Motor Neurons with Neuromuscular Junctions, Biochip Journal, 13(2): 127-132 (2019)

64 Imai, K., Ikeda, A., Shimizu, K., Honda, H.: Selective elimination of bitter peptides by adsorption to heat-treated porous silica gel, Food Science and Technology Research, 25(2): 179-186 (2019)

63 Ito, M., Shimizu, K., Honda, H.: Searching for high-binding peptides to bile acid for inhibition of intestinal cholesterol absorption using principal component analysis, Journal of Bioscience and Bioengineering, 127(3): 366-371 (2019)

62 Mizukami, Y., Takahashi, Y., Shimizu, K., Konishi, S., Takakura, Y., Nishikawa, M.: Regulation of the distribution of cells in mixed spheroids by altering migration direction, Tissue Engineering, Part A, 25(5-6): 390-398 (2019)

2018

61 Nagashima, T., Shimizu, K., Matsumoto, R., Honda, H.: Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine, Scientific Reports, 8:12427 (2018)

60 Imai, K., Shimizu, K., Kamimura, M., Honda, H.: Interaction between porous silica gel microcarriers and peptides for oral administration of functional peptides, Scientific Reports, 8:10971 (2018)

59 Imai, Y., Yoshida, K., Matsumoto, M., Okada, M., Kanie, K., Shimizu, K., Honda, H., Kato, R.: In-process evaluation of culture errors using morphology-based image analysis, Regenerative Therapy, 9:15-23 (2018)

58 Haraguchi, A., Fuchigami, Y., Kawaguchi, M., Fumoto, S., Ohyama, K., Shimizu, K., Hagimori, M., Kawakami, S.: Determining transgene expression characteristics using a suction device with multiple hole adjusting a left lateral

lobe of the mouse liver, Biological Pharmaceutical Bulletin, Accepted

57 Tanaka, Y., Nishikawa, M., Mizukami, Y., Kusamori, K., Ogino, Y., Nishimura, S., Shimizu, K., Konishi, S., Yakahashi, Y., Takakura, Y.: Control of polarization and tumoricidal activity of macrophages by multicellular spheroid formation, Journal of Controlled Release, Accepted

56 Hirokawa, Y., Kanesaki, Y., Arai, S., Saruta, F., Hayashihara, K., Murakami, A., Shimizu, K., Honda, H., Yoshikawa, H., Hanai, T.: Mutations responsible for alcohol tolerance in the mutant of Synechococcus elongatus PCC 7942 (SY1043) obtained by single-cell screening system, Journal of Bioscience and Bioengineering, Accepted

2017

55 Shimizu, K., Genma, R., Goto, Y., Nagasaka, S., Honda, H.: Three-dimensional cultured skeletal muscle tissue atrophy model induced with dexamethasone, Bioengineering, Accepted

54 Fujitani, M., Huddin, N.S., Kawai, S., Kanie, K., Kiyota, Y., Shimizu, K., Honda, H., Kato, R.: Morphology-based non-invasive quantitative prediction of the differentiation status of neural stem cells,  Journal of Bioscience and Bioengineering, Accepted

Excellent Paper Award of the Society for Biotechnology, Japan.

53 Oyama, N., Fuchigami, Y., Fumoto, S., Sato, M., Hagimori M., Shimizu, K., Kawakami, S.: Characterization of transgene expression and pDNA distribution in the kidney by renal suction-mediated transfection method in mice, Drug Delivery, Accepted

52 Nishikawa, T., Tanaka, Y., Kusamori, K., Mizuno, N., Mizukami, Y., Ogino, Y., Shimizu, K., Konishi, S., Takahashi, Y., Takakura, Y., Nishikawa, M.: Using size-controlled multicellular spheroids of murine adenocarcinoma cells for efficient establishment of pulmonary tumors in mice, Biotechnology Journal, Accepted

51 Arai, S., Hayashihara, K., Kanamoto, Y., Shimizu, K., Hirokawa, Y., Hanai, T., Murakami, A., Honda, H.: Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC7942 obtained by UV-C induced random mutagenesis and single cell screening system, Biotechnology and Bioengineering, Accepted

50 Kozaki I., Shimizu, K., Honda, H.: Effective modification of cell death-inducing intracellular peptides by means of a photo-cleavable peptide array-based screening system, Journal of Bioscience and Bioengineering, Accepted

49 Nagasaka, R., Gotou, Y., Yoshida, K., Kanie, K., Shimizu, K., Honda, H., Kato, R.: Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells, Journal of Bioscience and Bioengineering, 123(5):642-650 (2017)

48 Nishikawa T, Tanaka Y, Nishikawa M, Ogino Y, Kusamori K, Mizuno N, Mizukami Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y.: Optimization of Albumin Secretion and Metabolic Activity of Cytochrome P450 1A1 of Human Hepatoblastoma HepG2 Cells in Multicellular Spheroids by Controlling Spheroid Size, Biological Pharmaceutical Bulletin, 40(3):334-338 (2017)

47 Kume, A., Kawai, S., Kato, R., Iwata, S., Shimizu, K., Honda, H.: Exploring high-affinity binding properties of octamer peptides by principal component analysis of tetramer peptides, Journal of Bioscience and Bioengineering, 123(2):230-238 (2017)

2016

46 Yamamoto, S., Shimizu, K., Fei, J., Iwata, H., Okochi, M., Nakanishi, H., Honda, H.: Ex vivo culture of circulating tumor cells using magnetic force-based coculture on a fibroblast feeder layer, Biotechnology Journal, 11(11): 1433-1442 (2016)

45 Matsumoto, R., Shimizu, K., Nagashima, T., Tanaka, H., Mizuno, M., Kikkawa, F., Hori, M., Honda, H.: Plasma-Activated Medium Selectively Eliminates Undifferentiated Human Induced Pluripotent Stem Cells, Regenerative therapy, 5:55-63 (2016)

44 Kume, A., Okochi, M., Shimizu, K., Yoshida, Y., Honda, H.: Development of a tactical screening method to investigate the characteristics of functional peptides, Biotechnology and Bioprocess Engineering, 21(1): 119-127 (2016)

43 Taniguchi, Y., Kawakami, S., Fuchigami, Y., Oyama, N., Yamashita, F., Konishi, S., Shimizu, K., Hashida, M.: Optimization of renal transfection using a renal suction-mediated transfection method in mice, Journal of Drug Targeting, 24(5): 450-456 (2016)

42 Kusamori, K., Nishikawa, M., Mizuno, N., Nishikawa, T., Masuzawa, A., Tanaka, Y., Mizukami, Y., Shimizu, K., Konishi, S., Takahashi, Y., Takakura, Y.: Increased insulin secretion from insulin-secreting cells by construction of mixed multicellular spheroids, Pharmaceutical Research, 33(1): 247-256 (2016)

Selected as a cover image. 

41 Arai, S., Okochi, M., Shimizu, K., Hanai, T., Honda, H.: A Single Cell Culture System Using Lectin-Conjugated Magnetite Nanoparticles and Magnetic Force to Screen Mutant Cyanobacteria, Biotechnology and Bioengineering, 113(1): 112-119 (2016)

2015

40 Matsumoto, R., Okochi, M., Shimizu, K., Kanie, K., Kato, R., Honda, H.: Effects of the properties of short peptides conjugated with cell-penetrating peptides on their internalization into cells, Scientific Reports, 5: 12884 (2015)

39 Yamamoto, S., Fei, J., Okochi, M., Shimizu, K., Yusa, A., Kondo, N., Iwata, H., Nakanishi, H., Honda, H.: Efficient capturing of circulating tumor cells using a magnetic capture column and a size-selective filter, Bioprocess and Biosystems Engineering, 38(9): 1693-1704 (2015)

38 Shimizu, K., Araki, H., Sakata, K., Tonomura, W., Hashida, M., Konishi, S.: Microfluidic Devices for Construction of Contractile Skeletal Muscle Microtissues, Journal of Bioscience and Bioengineering, 119(2): 212-216 (2015)

Recommended on Pubadvanced

2014

37 Ukawa, M., Akita, H., Hayashi, Y., Ishiba, R., Tange, K., Arai, M., Kubo, K., Higuchi, Y., Shimizu, K., Konishi, S., Hashida, M., Harashima, H.: Neutralized nanoparticle composed of SS-cleavable and pH-activated lipid-like material as a long-lasting and liver-specific gene delivery system, Advanced Healthcare Materials, 3(8): 1222-1229 (2014)

36 Shimizu, K., Zhang, G., Kawakami, S., Taniguchi, Y., Hayashi, K., Hashida, M., Konishi S.: Liver suction-mediated transfection in mice using a pressure-controlled computer system, Biological & Pharmaceutical Bulletin, 37(4): 569-575 (2014) 

Highlighted paper selected by Editor-in-Chief

35 Kusamori, K., Nishikawa, M., Mizuno, N., Nishikawa, T., Masuzawa, A., Shimizu, K., Konishi, S., Takahashi, Y., Takakura, Y.: Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice, Journal of Controlled Release, 173: 119-124 (2014)

2013 

34 Shimizu, K., Kusamori, K., Nishikawa, M., Mizuno, N., Nishikawa, T., Masuzawa, A., Katano, S., Takahashi, Y., Takakura, Y., Konishi, S.: Poly(N-isopropylacrylamide)-Coated Microwell Arrays for Construction and Recovery of Multicellular Spheroids, Journal of Bioscience and Bioengineering, 115(6): 695-699 (2013) 

Review 1 

Shimizu, K., Fujita, H., Nagamori, E.: Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators, Journal of Bioscience and Bioengineering, 115(2): 115-121 (2013)

Featured as a Key Scientific Articles on Global Medical Discovery [ISSN 1929-8536]

33 Nagamori, E., Shimizu, K., Fujita, H., Tokuhiro, K., Ishida, N., Takahashi, H.: Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions, Bioprocess and Biosystems Engineering, 36: 1261-1265 (2013) 

32 Nagamori, E., Fujita, H., Shimizu, K., Tokuhiro, K., Ishida, N., Takahashi, H.: Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter, Journal of Bioscience and Bioengineering, 115(2): 193-195 (2013)

2012 

31 Shimizu, K., Kawakami, S., Hayashi, K., Kinoshita, H., Kuwahara, K., Nakao, K., Hashida, M., Konishi, S.: In vivo site-specific transfection of naked plasmid DNA and siRNAs in mice by using a tissue suction device, PLoS ONE, 7(7): e41319 (2012)

30 Shimizu, K., Kawakami, S., Hayashi, K., Mori, Y., Hashida, M., Konishi, S.: Implantable Pneumatically Actuated Microsystem for Renal Pressure-Mediated Transfection in Mice, Journal of Controlled Release, 159: 85-91 (2012)

29 Kobayashi, T., Shimizu, K., Konishi, S.: Investigation of Denaturation of Hydrophobic Perfluoropolymer Surfaces and Their applications for Micropatterns on Biochip, IEEE/ASME Journal of Micoelectromechanical Systems, 21(1): 62-67 (2012) 

2011

28 Ishii, M., Shibata, R., Numaguchi, Y., Kito, T., Suzuki, H., Shimizu, K., Ito, A., Honda, H., Murohara, T.: Enhanced Angiogenesis by Transplantation of Mesenchymal Stem Cell Sheet Created by a Novel Magnetic Tissue Engineering Method, Arterioeclerosis Thrombosis and Vascular Biology, 31(10): 2210-2215 (2011)

27 Shimizu. K., Higuchi, Y., Kozu, Y., Hashida, M., Konishi, S.: Development of a Suction Device for Stabilizing in vivo Real-Time Imaging of Murine Tissues,  Journal of Bioscience and Bioengineering, 112 (5): 508-510 (2011）

26 Shimizu, K., Shunori, A., Morimoto, K., Hashida, M., Konishi, S.: Development of a Biochip with Serially Connected Pneumatic Balloons for Cell-stretching Culture, Sensors and Actuators B: Chemical, 156: 486?493 (2011)

25 Kobayashi, T., Shimizu, K., Kaizuma, Y., Konishi, S.: Formation of Superhydrophobic/Superhydrophilic Patterns by Combination of Nanostructure-Imprinted Perfluoropolymer and Nanostructured Silicon Oxide for Biological Droplet Generation. Applied Physics Letters, 98: 123706 (2011)

 Selected as an article in Virtual Journal of Biological Physics Research, 21(7) by AIP and APS

24 Kobayashi, T., Shimizu, K., Kaizuma, Y., Konishi, S.: Novel Combination of Hydrophilic/Hydrophobic Surface for Large Wettability Difference and Its Application to Liquid Manipulation. Lab on a Chip, 11(4): 639-644 (2011)

23 "Fujita, H., Dau, V.T., Shimizu, K., Hatsuda, R., Sugiyama, S., Nagamori, E.: Designing of a Si-MEMS device with an integrated skeletal muscle cell-based bio-actuator. Biomedical Microdevices, 13: 123-129 (2011) " 

2010

22 "Fujita, H., Hirano, M., Shimizu, K., Nagamori, E.: Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise. Journal of Muscle Research and Cell Motility, 31: 279-288 (2010)" 

21 "Fujita, H., Endo, A., Shimizu, K., Nagamori, E.: Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability. Biotechnology and Bioengineering, 107(5): 894-901 (2010)" 

20 "Fujita, H., Shimizu, K., Morioka, Y., Nagamori, E.: Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery. Journal of Bioscience and Bioengineering, 110(3): 359-362 (2010)" 

19 "Fujita, H., Shimizu, K., Nagamori, E.: Novel method for measuring active tension generation by C2C12 myotube using UV-crosslinked collagen film. Biotechnology and Bioengineering, 106(3):482-489 (2010).

 Selected as a cover image 

18 "Shimizu, K., Fujita, H., Nagamori, E.: Oxygen plasma-treated thermoresponsive polymer surfaces for cell sheet engineering. Biotechnology and Bioengineering, 106(2):303-310 (2010) " 

17 "Shimizu, K., Sasaki, H., Hida, H., Fujita, H., Obinata, K., Shikida, M., Nagamori, E.: Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement. Biomedical microdevices, 12:247-252 (2010)" 

16 "Fujita, H., Shimizu, K., Yamamoto, Y., Ito, A., Kamihira, M., Nagamori, E.: Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells. Journal of Tissue Engineering and Regenerative Medicine, 4:437-443 (2010)" 

15 "Shimizu, K., Fujita, H., Nagamori, E.: Micropatterning of Single Myotubes on a Thermoresponsive Culture Surface using Elastic Stencil Membranes for Single-Cell Analysis. Journal of Bioscience and Bioengineering, 109(2):174-178 (2010)"

2009

14 "Yamamoto, Y., Ito, A., Kato, M., Kawabe, Y., Shimizu, K., Fujita, H., Nagamori, E., Kamihira, M.: Preparation of artificial skeletal muscle tissues by a magnetic force-based tissue engineering technique. Journal of Bioscience and Bioengineering, 108(6):538-543 (2009)" 

13 "Fujita, H., Shimizu, K., Nagamori, E.: Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V. Biotechnology and Bioengineering, 103(5):1034-1041 (2009)

 Spotlighted in this issue

12 "Shimizu, K., Fujita, H., Nagamori, E.: Alignment of skeletal muscle myoblasts and myotubes using linear micropatterned surfaces ground with abrasives. Biotechnology and Bioengineering, 103(3):631-638 (2009) " 

11 "Fujita, H.*, Shimizu, K.*, Nagamori, E.: Application of a cell sheet-polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability. Biotechnology and Bioengineering, 103(2):370-377 (2009) *These authors contributed equally to this work. " 

2008

10 "Okochi, M., Kurimoto, M., Shimizu, K., Honda, H.: Effect of global transcriptional regulators related to carbohydrate metabolism on organic solvent tolerance in Escherichia coli. Journal of Bioscience and Bioengineering, 105(4):389-394 (2008)

2007

9 "Shimizu, K., Ito, A., Honda, H.: Mag-seeding of rat bone marrow cells into porous hydroxyapatite scaffolds for bone tissue engineering. Journal of Bioscience and Bioengineering, 104(3):171-177 (2007)" 

8 "Shimizu, K., Ito, A., Arinobe, M., Murase, Y., Iwata, Y., Narita, Y., Kagami, H., Ueda, M., Honda, H.: Effective cell-seeding using magnetite nanoparticles and magnetic force onto decellularized blood vessel for vascular tissue engineering. Journal of Bioscience and Bioengineering, 103(5):472-478 (2007).

 Excellent Paper Award of the Society for Biotechnology, Japan.

7 "Shimizu, K., Ito, A., Yoshida, T., Yamada, Y., Ueda, M., Honda, H.: Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force. Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 82(2):471-480 (2007) " 

6 "Okochi, M., Kurimoto, M., Shimizu, K., Honda, H.: Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli. Applied Microbiology and Biotechnology, 73(6):1394-1399 (2007)" 

5 "Shimizu, K., Ito, A., Lee, J.K., Yoshida, T., Miwa, K., Ishiguro, H., Numaguchi, Y., Murohara, T., Kodama, I., Honda, H.: Construction of multilayered cardiomyocyte sheets using magnetite nanoparticles and magnetic force. Biotechnology and Bioengineering, 96(4):803-809. (2007).

Top 25 downloaded articles from September ? December 2007

2006

4 "Shimizu, K., Ito, A., Honda, H.: Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles. Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 77B:265-272 (2006)" 

2005

3 "Ito, A., Hibino, E., Shimizu, K., Kobayashi T., Yamada Y., Hibi H., Ueda M., Honda H.: Magnetic force-based mesenchymal stem cell expansion using antibody conjugated magnetoliposomes. Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 75(2):320-327 (2005)" 

2 "Shimizu, K., Hayashi, S., Doukyu, N., Tsuakagoshi, N., Kobayashi, T., and Honda, H.: Discovery of glpC, an organic solvent tolerance related gene in Escherichia coli, using gene expression profiles from DNA microarrays. Applied and Environmental Microbiology, vol. 71, No. 2, 1093-1096 (2005)" 

1 "Shimizu, K., Hayashi, S., Doukyu, N., Kobayashi, T., and Honda, H.: Time-Course Data Analysis of Gene Expression Profiles Reveals purR Regulon Concerns in Organic Solvent Tolerance in Escherichia coli. Journal of Bioscience and Bioengineering, vol. 99, No. 1, 72-74 (2005)"