Dear Biochemistry Experiment students
Hi!
This is head TA, Junwoo Lee.
I want to notice two important information to you.
1.
You need to come to #601, Gung-li Dong to check your transformation result.
Up to tomorrow noon, head TA will send a text message to TAs the result of transformation.
And from 7:00PM to 9:00PM, next Monday [17th, September], every students need to come to #601, Gung-li Dong to check your transformation result with the help of TAs.
If you cannot come to lecture room at that time, you should make a reservation with your TA to check the result at TA's lab.
If you fail to get colonies from your plate, you need to perform the experiment once more since you need to take a plate picture to verify your successful performance for vector transformation.
2.
In order to perform mini-prep experiment, which is widely used to extract and purify plasmid from transformed E.coli, you need to inoculate your transformed E.coli colony in LA [LB medium with Ampicillin].
So, please make a reservation with your TA on Thursday, 20th, September to perform this inoculation process ['seeding', alternatively].
You will pick single colony from your LA plate and inoculate it into ~2mL of fresh LA liquid medium. This mix will be incubated at 37 Celsius degree shaking incubator for over-night [more than 12hr].
And I will upload about the result of your HW and Quiz up to next Thursday (20th, September).
Plz check your point.
Thank you.