Protocols
This collection of experimental protocols for Helobdella (leech) developmental biology is maintained by Dian-Han Kuo and members of his lab at Department of Life Science, National Taiwan University. Some protocols are already published in Cold Spring Harbor Protocols. Please visit CSHLP's websites for these contents (for links, see below). Updates and comments on these published protocols are also provided on this website.
Handling of Helobdella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5189.
[PubMed] [doi:10.1101/pdb.prot5189] [additional info]
Microinjection of Helobdella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5190.
[PubMed] [doi:10.1101/pdb.prot5190] [additional info]
Devitellinization of living Helobdella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5191.
[PubMed] [doi:10.1101/pdb.prot5191] [additional info]
Arnolds' silver staining of Helbodella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5191.
[PubMed] [doi:10.1101/pdb.prot5192] [additional info]
Immunostaining Helobdella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5193.
[PubMed] [doi:10.1101/pdb.prot5193] [additional info]
In situ hybridization of Helobdella (leech) embryos. Cold Spring Harb. Protoc. 2009(4):pdb.prot5194.
[PubMed] [doi:10.1101/pdb.prot5194] [additional info]
Whole-mount preparation of Helobdella (leech) embryos for microscopy. Cold Spring Harb. Protoc. 2009(4):pdb.prot5195.