Pressure microinjection [see the CSH Protocols chapter for the setup of the injection rig]

• Dextran tracer
• mRNA & plasmid DNA
• morpholino oligo
• DNase I
• ricin A chain
• other possible injectants: calcium indicator FURA-2 (Molecular Probes F6799), pH indicators SNARF-1 dextran (Molecular Probes D3303) or BCECF (Molecular Probes D1878), live mitochondrial marker rhodamine 123 (Molecular Probes R302).

Iontophoretic microinjection