Publications

Selected Publications

10. Jing-Jing Liu, Guo-Chang Zhang, In Iok Kong, Eun Ju Yun, Jia-Qi Zheng, Dae-Hyuk Kweon, Yong-Su Jin*. 2018. " A mutation in PGM2 causing inefficient galactose metabolism by the probiotic yeast Saccharomyces boulardii" Applied and Environmental Microbiology. (Accepted manuscript posted online 9 March 2018, doi:10.1128/AEM.02858-17). View pape


Schematic overview of galactose utilization and Pgm2 inactivation induced galactose toxicity in S. boulardii.

9. Guo-Chang Zhang, Timothy L. Turner, Yong-Su Jin*. 2017. "Enhanced xylose fermentation by engineered yeast expressing NADH oxidase through high cell density inoculums". Journal of Industrial Microbiology & Biotechnology 3: 387–395. View paper


Schematic of noxE interacting with glucose and xylose metabolism. While noxEexpression in yeast can resolve the redox imbalance issue of xylose fermentation, it also causes growth defects. To bypass this problem, high inoculum or a mixture of glucose and xylose can be used. F1,6p fructose-1,6-bisphosphate; PPP pentose phosphate pathway; GA3P glyceraldehyde-3-phosphate; DHAP dihydroxyacetone phosphate

8. Peng-Fei Xia, Guo-Chang Zhang, Berkley Walker, Seung-Oh Seo, Suryang Kwak, Jing-Jing Liu, Heejin Kim, Donald R Ort, Shu-Guang Wang, Yong-Su Jin*. 2016. "Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae". ACS Synthetic Biology 6 (2): 276–283. View paper

7. Jing-Jing Liu, Guo-Chang Zhang, Eun Joong Oh, Panchalee Pathanibul, Timothy L Turner, Yong-Su Jin*. 2016. "Lactose fermentation by engineered Saccharomyces cerevisiae capable of fermenting cellobiose" Journal of Biotechnology 234:99-104. View paper

Diagram of lactose-consuming strain carrying multi-copies of lactose transporter, CDT-1 and β-glucosidase/β-galactosidase, GH1-1

6. Peng-Fei Xia, Guo-Chang Zhang, Jing-Jing Liu, Suryang Kwak, Ching-Sung Tsai, In Iok Kong, Bong Hyun Sung, Jung-Hoon Sohn, Shu-Guang Wang, Yong-Su Jin*. 2016. "GroE chaperonins assisted functional expression of bacterial enzymes in Saccharomyces cerevisiae" Biotechnology and Bioengineering 113:2149-2155. View paper

This study demonstrated that the mismatching of HSP60 chaperonins between bacteria and yeast is the reason that some bacterial enzymes cannot be functionally expressed in Saccharomyces cerevisiae. A post‐translational tool is further developed to facilitating the functional expression of bacterial enzymes in S. cerevisiae via co‐expression of bacterial groE chaperonins.

5. Timothy L Turner, Guo-Chang Zhang, Soo Rin Kim, Vijay Subramaniam, David Steffen, Christopher D Skory, Ji Yeon Jang, Byung Jo Yu, Yong-Su Jin*. 2015. "Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion" Applied microbiology and biotechnology 99:8023-8033. View paper

Metabolic pathway for the engineered SR8L xylose-utilizing yeast with a heterologous lactic acid pathway. XR, XDH, and XK were previously expressed, and ald6 and pho13were deleted to allow for efficient xylose utilization and minimization of acetate production. In this study, ldhA, which encodes for lactate dehydrogenase (LDH), was introduced to allow for lactic acid production from xylose or glucose

4. Guo-Chang Zhang, In Iok Kong, Na Wei, Dairong Peng, Yong-Su Jin*. 2015. "Metabolic engineering of an acetate reduction pathway for producing cellulosic ethanol". Biotechnology and Bioengineering 113:2587-2596. View paper


Illustration of the xylose and acetate co‐consumption pathway in engineered S. cerevisiae. ACS*, AADH, XR, and XDH stand for mutant acetyl‐CoA synthetase from Salmonella enterica, acetylating acetaldehyde dehydrogenase from E. coli, xylose reductase, and xylitol dehydrogenase from Scheffersomyces stipitis, respectively. The most likely rate‐limiting factors (ACS*, AADH, ATP, and NADH) for acetate consumption are in bold.

3. Guo-Chang Zhang, Jing-Jing Liu, In Iok Kong, Suryang Kwak, Yong-Su Jin*. 2015. "Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion" Current Opinion in Chemical Biology 29:49-57. View paper

• Co-fermentation of mixed C6 and C5 sugars enabled efficient bioconversion.

• Metabolic pathways and transporters for non-glucose sugars have been identified.

• Engineering of model hosts for co-utilization of C6 and C5 has been demonstrated.

2. Guo-Chang Zhang, In Iok Kong, Heejin Kim, Jing-Jing Liu, Jamie HD Cate, Yong-Su Jin*. 2014. "Construction of a Quadruple Auxotrophic Mutant of an Industrial Polyploid Saccharomyces cerevisiae Strain by Using RNA-Guided Cas9 Nuclease" Applied and Environmental Microbiology 80:7694-7701. View paper


CRISPR-Cas9-mediated gene disruption in polyploid yeast. (A) Diagram of CRISPR-Cas-directed gene disruption. (B) Flow chart for disruption of the auxotrophic genes in the polyploid industrial yeast strain S. cerevisiae ATCC 4124.

1. Guo-chang Zhang, Jing-jing Liu*, Wen-tao Ding. 2012. "Decreased Xylitol Formation during Xylose Fermentation of Saccharomyces cerevisiae Due to Overexpression of Water-Forming NADH Oxidase." Applied and Environmental Microbiology 78:1081-1086. View paper

Glucose and xylose metabolism pathway in recombinant Saccharomyces cerevisiae. The key enzymes and the cofactor requirements that were identified in the central metabolism are shown. Abbreviations: XR, xylose reductase; XDH, xylitol dehydrogenase; XK, xylulokinase; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; F1,6P, fructose-1,6-bisphosphate; PPP, pentose phosphate pathway; GA3P, glyceraldehyde-3-phosphate; DHAP, dihydroxyacetone phosphate; AcCoA, acetylcoenzyme A; TCA, tricarboxylic acid cycle.