Publications
Selected Publications
Transition transferases prime bacterial capsule polymerization. Litschko C, Di Domenico V, Schulze J, Sizhe L, Ovchinnikova OG, Voskuilen T, Bethe A, Cifuente JO, Marina A, Budde I, Mast TA, Sulewska M, Berger M, Buettner FFR, Lowary TL, Whitfield C, Codee JDC, Schubert M, Guerin ME*, Fiebig T. Nat. Chem. Biol. doi: 10.1038/s41589-024-01664-8 (2024).
The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan relase and stimulates growth. Franklin A, Layton AJ, Mize T, Salgueiro VC, Sullivan R, Benedict ST, Gurcha SS, Anso I, Besra GS, Banzhaf M, Lovering AL, Williams SJ, Guerin ME, Scott NE, Prados-Rosales R, Lowe E, Moynihan PJ. Nat. Commun. 15:5740 (2024).
Human gut microbes express functionally distinct endoglycosidases to metabolize the same N-glycan structure. Sastre DE, Sultana N, Navarro VAS, Huliciak M, Du J, Cifuente JO, Flowers M, Liu X, Lollar P, Trastoy B, Guerin ME, Sundberg EJ. Nat. Commun. 15:5123 (2024).
Architecture, function, regulation and evolution of α-glucans metabolic enzymes in prokaryotes. Cifuente JO, Colleoni C, Kalscheuer R, Guerin ME*. Chem. Rev. 124(8):4863-4934 (2024). Review.
Acylation of glycerolipids in mycobacteria. Angala SH, Carreras-González A, Huc-Claustre E, Anso I, Kaur D, Jones V, Palcekova Z, Belardinelli JM, de Sousa-d'Auria C, Shi L, Slama N, Houssin C, Quemard A, McNeil M, Guerin ME, Jackson M. Nat. Commun. 14:6694 (2023).
CryoEM analysis of the essential native UDP-glucose pyrophosphorylase from Aspergillus nidulans reveals key conformations for activity regulation and function. Han X, D'Angelo C, Otamendi A, Cifuente JO, Astigarraga E, Ochoa-Lizarralde B, Grininger M, Routier FH, Guerin ME, Fuehring J, Etxebeste O, Connell SR. mBio 14(4):e0041423 (2023).
Trapping and retaining intermediates in glycosyltransferases. Guerin ME*. J. Biol. Chem. 299(8):105006 (2023).
A multi-enzyme machine polymerizes the Haemophilus influenzae type b capsule. Cifuente JO, Schulze J, Bethe A, Di Domenico V, Litschko C, Budde I, Eidenberger L, Thiesler H, Ramón-Roth I, Berger M, D'Angelo C, Marina A, Gerardy-Schahn R, Schubert M, Guerin ME*, Fiebig T. Nat. Chem. Biol. 19:865-877 (2023). Highlighted in the News and Views section as Basket of sweets. Dorfmueller HC. Nat. Chem. Biol. 19:795-796 (2023).
Turning universal O into rare Bombay type blood. Anso I, Naegeli A, Cifuente JO, Orrantia A, Andersson E, Zenarruzabeitia O, Moraleda-Montoya A, Garcia-Alija M, Corzana F, Del Orbe RA, Borrego F, Trastoy B, Sjogreen J, Guerin ME*. Nat. Commun. 14:1765 (2023).
Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases. Trastoy B, Du JJ, Cifuente JO, Rudolph L, García-Alija M, Klontz E, Deredge D, Sultana N, Huynh CG, Flowers M, Li C, Sastre D, Wang LX, Corzana F, Mallagaray A, Sundberg EJ, Guerin ME*. Nat. Commun. 14:1705 (2023). Highlighted as Faculty Opinions article.
Modulating antibody effector functions by Fc glycoengineering. García-Alija M, van Moer B, Sastre DE, Azzam T, Du JJ, Trastoy B, Callewaert N, Sundberg EJ, Guerin ME*. Biotechnol. Adv. doi:10.1016/j.biotechadv.2023.108201 (2023). Review.
SigH stress response mediates killing of Mycobacterium tuberculosis by activating nitrophuran prodrugs via induction of Mrx2 expression. Cioetto-Mazzabó, Boldrin F, Beauvineau C, Speth M, Marina A, Namouchi A, Segafreddo G, Cimino M, Favre-Rochex S, Balasingham S, Trastoy B, Munier-Lehmann H, Griffiths G, Gicquel B, Guerin ME, Manganelli R, Alonso-Rodriguez N. Nucleic Acids Res. 51(1):144-165 (2023).
Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum. Cordeiro RL, Santos CR, Domingues MN, Lima TB, Pirolla RAS, Morais MAB, Colombari FM, Miyamoto RY, Persinoti GF, Borges AC, de Farias MA, Stoffel F, Li C, Gozzo FC, van Heel M, Guerin ME, Sundberg EJ, Wang LX, Portugal RV, Giuseppe PO, Murakami MT. Nat. Chem Biol. 19(2):218-229 (2023). Highlighted in the News and Views section as A sweet feast. Crouch LI. Nat. Chem. Biol. 19(2):131-132 (2023).
Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE. García-Alija M, Du JJ, Ordoñez I, Diz-Vallenilla A, Moraleda-Montoya A, Sultana N, Huynh CG, Li C, Donahue TC, Wang LX, Trastoy B, Sundberg EJ, Guerin ME*. Nat. Commun. 13:1137 (2022). Featured article by Nat. Commun. Editors.
Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases. Trastoy B, Du JJ, García-Alija M, Li C, Klontz EH, Wang LX, Sundberg EJ, Guerin ME*. Curr. Opin. Struct. Biol. 72:248-259 (2022). Highlighted as Faculty Opinions article.
Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA. Anso I, Basso LGM, Wand L, Marina A, Páez-Perez ED, Jager C, Gavotto F, Tersa M, Perrone S, Contreras FX, Prandi J, Gilleron M, Linster CL, Corzana F, Lowary TL, Trastoy B, Guerin ME*. Sci. Adv. 7:eabj4565 (2021).
GH18 endo-β-N-acetylglucosaminidases use distinct mechanisms to process hybrid-type N-linked glycans. Trastoy B, Du, JJ, Li, C, Garcia-Alija M, Klontz EH, Roberts BR, Donahue TC, Wang LX, Sundberg EJ, Guerin ME*. J. Biol. Chem. 297:101011 (2021).
The phosphatidyl-myo-inositol dimannoside acyltransferase PatA is essential for Mycobacterium tuberculosis growth in vitro and in vivo. Boldrin F, Anso I, Alebouyeh S, Sevilla IA, Geijo M, Garrido JM, Marina A, Cioetto Mazzabo L, Segafreddo G, Guerin ME, Manganelli R, Prados-Rosales R. J. Bacteriol. 203:e00439-20 (2021).
Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila. Trastoy B, Naegeli A, Anso I, Sjogren J, Guerin ME*. Nat. Commun. 11:4844 (2020).
Unveiling the activation dynamics of a fold-switch bacterial glycosyltransferase by 19F NMR. Liebau J, Tersa M, Trastoy B, Patrick J, Rodrigo-Unzueta A, Corzana F, Sparrman T, Guerin ME*, Maler L. J. Biol. Chem. 295:9868-9878 (2020).
Dissecting the structural and chemical determinants of the 'open-to-closed' motion in the mannosyltransferase PimA from mycobacteria. Rodrigo-Unzueta A, Ghirardello M, Urresti S, Delso I, Giganti D, Anso I, Trastoy B, Comino N, Tersa M, D'Angelo C, Cifuente J, Marina A, Liebau J, Mäler L, Chenal A, Albesa-Jove D, Merino P, Guerin ME*. Biochemistry 59:2934-2945 (2020).
Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. Trastoy B, Du JJ, Klontz EH, Li C, Cifuente JO, Wang L-X, Sundberg EJ, Guerin ME*. Nat. Commun. 11:899 (2020). Highlighted as F1000 Prime article.
The allosteric control mechanism of bacterial glycogen biosynthesis disclosed by cryoEM. Cifuente JO, Comino N, D'Angelo C, Marina A, Gil-Cartón D, Albesa-Jové D, Guerin ME*. Curr. Res. Struct. Biol. 2:89-103 (2020).
Membrane fluidity adjusts the insertion of the transacylase PlsX to regulate phospholipid biosynthesis in Gram-positive bacteria. Sastre D, Basso LGM, Trastoy B, Cifuente JO, Contreras X, Gueiros-Filho F, de Mendoza D, Navarro MVAS, Guerin ME*. J. Biol. Chem. 295:2136-2147 (2020). Highlighted in the Lipid News section of ASBMB Today.
Structural insights into the mechanisms and specificities of IgG-active endoglycosidases. Du JJ, Klontz EH, Guerin ME, Trastoy B, Sundberg EJ. Glycobiology 30(4):268-279 (2020). Review.
Structure-function relationships underlying the dual N-acetylmuramic and GlcNAc specificities of the bacterial peptidoglycan deacetylase PdaC. Grifoll-Romero L, Sainz-Polo MA, Albesa-Jové D, Guerin ME, Biarnés X, Planas, A. J. Biol. Chem. 294(50):19066-19080 (2019).
Molecular basis of broad spectrum N-glycan specificity and processing of therapeutic IgG monoclonal antibodies by Endoglycosidase S2. Klontz, EH, Trastoy B, Deregde D, Fields JK, Li C, Orwenyo J, Marina A, Beadenkopf R, Gunther S, Flores J, Wintrode PL, Wang LX, Guerin ME*. Sundberg EJ. ACS Cent. Sci. 5(3):524-538 (2019). Highlighted as F1000 Prime article.
Structural basis of glycogen metabolism in bacteria. Cifuente JO, Comino N, Trastoy B, D'Angelo C, Guerin ME*. Biochem. J. 476(14):2059-2092 (2019). Review.
The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis. Kalscheuer R, Palacios A, Anso I, Cifuente JO, Anguita J, Jacobs WR, Guerin ME, Prados-Rosales R. Biochem. J. 476(14):1995-2016 (2019). Review.
Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases. Albesa-Jové D, Cifuente JO, Trastoy B, Guerin ME*. Methods Enzymol. 621(1):261-279 (2019). Review.
3-phosphoglycerate transhydrogenation instead of dehydrogenation alleviates the redox state dependency of yeast de novo L-serine synthesis. Paczia N, Becker-Kettern J, Conrotte JF, Cifuente JO, Guerin ME, Linster CL. Biochemistry. 58(4):259-275 (2019).
Regulation of human Hsc70 ATPase and chaperone activities by Apg2: role of the acidic subdomain. Cabrera Y, Dublang L, Fernández-Higuero JA, Albesa-Jové D, Lucas M, Viguera AR, Guerin ME, Vilar JMG, Muga A, and Moro F. J. Mol. Biol. 431:444-461 (2019).
Conformational entropy of a single peptide controlled under force governs protease recognition and catalysis. Guerin ME, Stirnemann G, and Giganti D. Proc. Natl. Acad. Sci. USA. 115(45):11525-11530 (2018).
Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Trastoy B, Klontz E, Orwenyo J, Marina A, Wang L-X, Sundberg EJ, and Guerin ME*. Nat. Commun. 9:1874 (2018).
The molecular mechanism of substrate recognition and catalysis of the membrane acyltransferase PatA from mycobacteria. Tersa M, Raich L, Albesa-Jové D, Trastoy B, Prandi J, Gilleron M, Rovira C, and Guerin ME*. ACS Chem. Biol. 13(1):131-140 (2018).
Structural snapshots of the reaction center of family GT6 α1,3-galactosyltransferase with native substrates. Insights into the catalytic mechanism of retaining glycosyltransferases. Albesa-Jové D, Sainz-Polo MA, Marina A, and Guerin ME*. Angew. Chem. int. Ed. Engl. 56(47):14853-14857 (2017).
Structural snapshots and loop dynamics along the catalytic cycle of glycosyltransferase GpgS. Albesa-Jové D, Romero-García J, Sancho-Vaello E, Contreras F-X, Rodrigo-Unzueta A, Comino N, Carreras-González A, Arrasate P, Urresti S, Biarnés X, Planas A, and Guerin ME*. Structure 25(7):1034-1044 (2017).
The antibacterial prodrug activator Rv2466c is a mycothiol-dependent reductase in the oxidative stress response of Mycobacterium tuberculosis. Rosado LA, Wahni K, De Giacomi G, Pedre B, Young D, de la Rubia AG, Boldrin F, Martens E, Marcos-Pascual L, Sancho-Vaello E, Albesa-Jové D, Provvedi R, Martin C, Makarov V, Versees W, Verniest G, Guerin ME, Mateos LM, Manganelli R, and Messens J. J. Biol. Chem. 292(32):13097-13110 (2017).
Mechanistic insights into the allosteric regulation of bacterial ADP-glucose pyrophosphorylases. Comino N, Cifuente JO, Marina A, Orrantia A, Eguskiza A, and Guerin ME*. J. Biol. Chem. 292(15):6255-6268 (2017).
Structural basis of phosphatidyl-myo-inositol mannosides biosynthesis in mycobacteria. Sancho-Vaello E, Albesa-Jové D, Rodrigo-Unzueta A, and Guerin ME*. BBA Mol. Cell. Biol. L. 1862(11):1355-1367. (2017). Review.
Structural basis of glycogen biosynthesis regulation in bacteria. Cifuente JO, Comino N, Madariaga-Marcos J, López-Fernández S, García-Alija M, Agirre J, Albesa-Jové D, and Guerin ME*. Structure 24:1613-1622 (2016).
The conformational plasticity of glycosyltransferases. Albesa-Jové D, and Guerin ME*. Curr. Opin. Struct. Biol. 40:23-32 (2016). Review.
Molecular basis of membrane association by the phosphatidylinositol mannosyltransferase PimA enzyme from mycobacteria. Rodrigo-Unzueta A, Martinez MA, Comino N, Alzari PM, Chenal A, and Guerin ME*. J. Biol. Chem. 291(27):13955-63 (2016).
Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA. Albesa-Jové D, Svetlikova Z, Tersa M, Sancho-Vaello E, Carreras-Gonzalez A, Bonnet P, Arrasate P, Eguskiza A, Angala SK, Cifuente JO, Kordulakova J, Jackson M, Mikusova K, and Guerin ME*. Nat. Commun. 7:10906 (2016).
The redox state regulates the conformation of Rv2466c to activate the anti tubercular prodrug TP053. Albesa-Jové D, Comino N, Tersa M, Mohorko E, Urresti S, Dainese E, Chiarelli LR, Pasca MR, Manganelli R, Makarov V, Riccardi G, Svergun DI, Glockshuber R, and Guerin ME*. J. Biol. Chem. 290(52):31077-31089 (2015).
A native ternary complex trapped in a crystal reveals the catalytic mechanism of a retaining glycosyltransferase. Albesa-Jové D, Mendoza F, Rodrigo-Unzueta A, Gomollon-Bel F, Cifuente JO, Urresti S, Comino N, Gomez H, Romero-Garcia J, Lluch JM, Sancho-Vaello E, Biarnés X, Planas A, Merino P, Masgrau L, and Guerin ME*. Angew. Chem. int. Ed. Engl. 54(34):9898-9902 (2015).
Secondary structure reshuffling modulates glycosyltransferase function at the membrane. Giganti D, Albesa-Jové D, Urresti S, Rodrigo-Unzueta A, Martínez MA, Comino N, Barilone N, Bellinzoni M, Chenal A, Guerin ME*, and Alzari PM. Nat. Chem. Biol. 11:16-18 (2015). Highlighted in the News and Views section as Membrane enzymes: Transformers at the interface. Brodhun F, Tittmann K. Nat. Chem. Biol. 11:102-103 (2015).
The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo. Boldrin F, Ventura M, Degiacomi G, Ravishankar S, Sala C, Svetlikova Z, Ambady A, Dhar N, Kordulakova J, Zhang M, Serafini A, Vishwas KG, Kolly GS, Kumar N, Palù G, Guerin ME, Mikusova K, Cole ST, Manganelli R. J. Bacteriol. 196(19):3441-3451 (2014).
Rv2466c mediates the activation of TP053 to kill replicating and non-replicating Mycobacterium tuberculosis. Albesa-Jové D, Chiarelli LR, Makarov V, Pasca MR, Urresti S, Mori G, Salina E, Vocat A, Comino N, Mohorko E, Ryabova S, Pfieiffer B, Lopes Ribeiro AL, Rodrigo-Unzueta A, Tersa M, Zanoni G, Buroni S, Altmann KH, Hartkoorn RC, Glockshuber R, Cole ST, Riccardi G, Guerin ME*. ACS Chem. Biol. 9(7):1567-1575 (2014). ACS Editors' Choice.
Structural basis of chitin oligosaccharide deacetylation. Andrés E, Albesa-Jové D, Biarnés X, Moerschbacher BM, Guerin ME*, Planas A. Angew. Chem. int. Ed. Engl. 53(27):6882-6887 (2014).
Structure-function relationships of membrane-associated GT-B glycosyltransferases. Albesa-Jové D, Giganti D, Jackson M, Alzari PM, Guerin ME*. Glycobiology 24(2):108-124 (2014). Review.
Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria. Giganti D, Alegre-Cebollada J, Urresti S, Albesa-Jové D, Rodrigo-Unzueta A, Comino N, Kachala M, López-Fernández S, Svergun DI, Fernández JM, Guerin ME*. J. Biol. Chem. 288(41):29797-29808 (2013).
Structural basis for feed-forward transcriptional regulation of membrane lipid homeostasis in Staphylococcus aureus. Albanesi D, Reh G, Guerin ME, Schaeffer F, Debarbouille M, Buschiazzo A, Schujman GE, de Mendoza D, Alzari PM. PLoS Pathog. 9(1):e1003108 (2013).
Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria. Urresti S, Albesa-Jové D, Schaeffer F, Pham HT, Kaur D, Gest P, van der Woerd MJ, Carreras-González A, López-Fernández S, Alzari PM, Brennan PJ, Jackson M, Guerin ME*. J. Biol. Chem. 287(29):24649-24661 (2012).
Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli. de la Paz Santangelo M, Gest PM, Guerin ME, Coinçon M, Pham H, Ryan G, Puckett SE, Spencer JS, Gonzalez-Juarrero M, Daher R, Lenaerts AJ, Schnappinger D, Therisod M, Ehrt S, Sygusch J, Jackson M. J. Biol. Chem. 286(46):40219-40231 (2011).
Rational design, synthesis, and evaluation of new selective inhibitors of microbial class II (zinc dependent) fructose bis-phosphate aldolases. Daher R, Coinçon M, Fonvielle M, Gest PM, Guerin ME, Jackson M, Sygusch J, Therisod M. J. Med. Chem. 53(21):7836-7842 (2010).
Molecular basis of phosphatidyl-myo-inositol mannoside biosynthesis and regulation in mycobacteria. Guerin ME*, Korduláková J, Alzari PM, Brennan PJ, Jackson M. J. Biol. Chem. 285(44):33577-33583 (2010). Review. Author Profile - www.jbc.org/content/285/44/33577/suppl/DC1
Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis. Kaur D, Guerin ME, Skovierová H, Brennan PJ, Jackson M. Adv. Appl. Microbiol. 69:23-78 (2009). Review.
New insights into the early steps of phosphatidylinositol mannoside biosynthesis in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis. Guerin ME*, Kaur D, Somashekar BS, Gibbs S, Gest P, Chatterjee D, Brennan PJ, Jackson M. J. Biol. Chem. 284(38):25687-25696 (2009).
Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis. Guerin ME*, Schaeffer F, Chaffotte A, Gest P, Giganti D, Korduláková J, van der Woerd M, Jackson M, Alzari PM. J. Biol. Chem. 284(32):21613-21625 (2009).
Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. Stadthagen G, Sambou T, Guerin ME, Barilone N, Boudou F, Korduláková J, Charles P, Alzari PM, Lemassu A, Daffé M, Puzo G, Gicquel B, Rivière M, Jackson M. J. Biol. Chem. 282(37):27270-27276 (2007).
Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria. Guerin ME, Kordulakova J, Schaeffer F, Svetlikova Z, Buschiazzo A, Giganti D, Gicquel B, Mikusova K, Jackson M, Alzari PM. J. Biol. Chem. 282(28):20705-20714 (2007).
Structural basis of lipid biosynthesis regulation in Gram-positive bacteria. Schujman GE*, Guerin ME*, Buschiazzo A, Schaeffer F, Llarrull LI, Reh G, Vila AJ, Alzari PM, de Mendoza D. EMBO J. 25(17):4074-4083 (2006).
Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation. Buschiazzo A, Ugalde JE, Guerin ME, Shepard W, Ugalde RA, Alzari PM. EMBO J. 23(16):3196-3205 (2004).
The UDP-glucose:glycoprotein glucosyltransferase is organized in at least two tightly bound domains from yeast to mammals. Guerin ME, Parodi AJ. J. Biol. Chem. 278(23):20540-20546 (2003).