Project outputs

Publications

1. A. Pereira, J. Trabulo, I. Fernandes, F. Cássio, C. Pascoal, S. Duarte (2016) Spring stimulates leaf decomposition in moderately eutrophic streams. Aquatic Sciences, https://doi.org/10.1007/s00027-016-0490-3

2. I. Fernandes, A. Pereira, J. Trabulo, C. Pascoal, F. Cássio, S. Duarte (2015) Microscopy- or DNA- based analyses: which methodology gives a truer picture of stream-dwelling decomposer fungal diversity? Fungal Ecology 18: 130-134. https://doi.org/10.1016/j.funeco.2015.08.005

3. S. Duarte, F. Bärlocher, J. Trabulo, F. Cássio, C. Pascoal (2015) Stream-dwelling fungal decomposer communities along a gradient of eutrophication unraveled by 454 pyrosequencing. Fungal Diversity 70: 127-148. https://doi.org/10.1007/s13225-014-0300-y 

4. S. Duarte, D. Batista, F. Bärlocher, F. Cássio, C. Pascoal (2015). Some new DNA barcodes of aquatic hyphomycete species. Mycoscience 56: 102-108. https://doi.org/10.1016/j.myc.2014.04.002 

5. S. Duarte, F. Bärlocher, F. Cássio, C. Pascoal (2014). Current status of DNA barcoding of aquatic hyphomycetes. Sydowia 66: 191-202. https://doi.org/10.12905/0380.sydowia66(2)2014-0191 

6. I. Fernandes, S. Duarte, F. Cássio, C. Pascoal (2013). Effects of riparian plant diversity loss on aquatic microbial decomposers become more pronounced with increasing time. Microbial Ecology, 66: 763-772 (partially supported by FunDiver). https://doi.org/10.1007/s00248-013-0278-0

7. S. Duarte, S. Seena, F. Bärlocher, C. Pascoal, F. Cássio (2013). A decade´s perspective on the impact of DNA sequencing on aquatic hyphomycete research. Fungal Biology Reviews,  27:19-24. https://doi.org/10.1016/j.fbr.2013.02.003

8. S. Duarte, S. Seena, F. Bärlocher, F. Cássio, C. Pascoal (2012). Preliminary insights into the phylogeography of six aquatic hyphomycete species. PLoS ONE 7(9):e45289. https://doi.org/10.1371/journal.pone.0045289 

9. S. Seena, S. Duarte, C. Pascoal, F. Cássio (2012). Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective. PLoS ONE 7(4):e35884. https://doi.org/10.1371/journal.pone.0035884 

10. S. Duarte, F. Cássio, C. Pascoal (2012). Denaturing gradient gel electrophoresis (DGGE) in microbial ecology – insights from freshwaters. In: Gel Electrophoresis – Principles and Basics, S. Magdeldin (Ed). InTech, Rijeka, Croatia, ISBN 979-953-307-474-9. https://doi.org/10.5772/38177 

Oral Communications 

1. S. Duarte. The impact of next generation sequencing on Microbial Ecology: insights from aquatic ecosystems. Advanced course in Genomics Metagenomics and Bioinformatics, Department of Biology, University of Minho, Braga, Portugal, July 12, 2016.

2. S. Duarte. Biogeography of stream-dwelling decomposer fungi: current knowledge and future perspectives. Plenary session in the XVIII Congress of the Iberian Association of Limnology (AIL), Tortosa, Spain, July 7, 2016.

3. S. Duarte. Assessing diversity of decomposer fungal communities in streams by 454 pyrosequencing. Advanced course in next generation sequencing, Department of Biology, University of Minho, Braga, Portugal, July 9, 2015.

4. S. Duarte. Diversity and activity of stream-dwelling decomposer fungi. Secondary school Camilo Castelo Branco, V. N. Famalicão, Portugal, January 27, 2015.

5. S. Duarte, J. Trabulo, F. Bärlocher, F. Cássio, C. Pascoal. Diversity of stream-dwelling fungal decomposer communities unravelled by 454 pyrosequencing. XVII Congress of the Iberian Association of Limnology, Santander, Spain, July 6-11, 2014.

6. A. Pereira, J. Trabulo, I. Fernandes, C. Pascoal, F. Cássio, S. Duarte. Seasonal effects of eutrophication on litter decomposition and associated fungal and invertebrate communities. XVII Congress of the Iberian Association of Limnology, Santander, Spain, July 6-11, 2014.

7. S. Duarte. The impact of DNA barcoding on aquatic hyphomycete research: preliminary studies. Advanced course on Microbial diversity: methods and applications, Department of Biology, University of Aveiro, Aveiro, Portugal, July 18, 2013.

8. S. Duarte, S. Seena, F. Bärlocher, C. Pascoal, F. Cássio. A decade’s perspective on the impact of DNA sequencing on aquatic hyphomycete research. Symposium for European Freshwater Sciences (SEFS 8), Münster, Germany, July 1-5, 2013.

9. I. Fernandes, S. Duarte, F. Cássio, C. Pascoal. Effects of riparian plant diversity loss on detritus food webs become more pronounced at longer times. Symposium for European Freshwater Sciences (SEFS 8), Münster, Germany, July 1-5, 2013 (partially supported by FunDiver).

10. S. Duarte. The impact of DNA barcoding on aquatic hyphomycete research: preliminary studies. DBio Seminars, Department of Biology, University of Minho, Braga, Portugal, April 10, 2013.

11. S. Duarte. Diversidade e atividade de fungos decompositores da folhada em rios: abordagens tradicionais e moleculares. XIV Jornadas de Biologia Aplicada – BioPercursos no século XXI, University of Minho, Braga, Portugal, February 27-28 and March 1, 2013.

12. S. Duarte, S. Seena, F. Bärlocher, F. Cássio, C. Pascoal. Biogeography of aquatic fungi: preliminary conclusions. XVI Congress of the Iberian Association of Limnology, Guimarães, Portugal, July 2-6, 2012.

Poster Communications

1. S. Duarte, J. Mora-Gómez, A. Romaní, F. Cássio, C. Pascoal. Eutrophication alters responses of stream-dwelling decomposers to drought. Detrital Dynamics Conference, London, UK, July 23-25, 2014 (partially supported by FunDiver).

2.  I. Fernandes, A. Pereira, J. Trabulo, C. Pascoal, F. Cássio, S. Duarte. A case of using traditional and molecular approaches to assess the effects of season and eutrophication on fungal communities on plant litter decomposing in streams. XVII Congress of the Iberian Association of Limnology, Santander, Spain, July 6-11, 2014.

3. D. Batista, C. Pascoal, F. Cássio, S. Duarte. Is intraspecific diversity of aquatic hyphomycetes on decomposing leaves in streams related to season? Symposium for European Freshwater Sciences (SEFS 8), Münster, Germany, July 1-5, 2013.

4. C. Pascoal, D. Batista, F. Cássio, S. Duarte. New DNA barcodes for 8 aquatic hyphomycete species. Symposium for European Freshwater Sciences (SEFS 8), Münster, Germany, July 1-5, 2013.

5. B. Antunes, J. Trabulo, S. Duarte, S. Seena, F. Cássio, C. Pascoal. Intraspecific diversity affects plant-litter decomposition in freshwaters. Poster presentation in the XIV Jornadas de Biologia Aplicada – BioPercursos no século XXI, Braga, Portugal, February 27-28 and March 1, 2013.

6. B. Antunes, J. Trabulo, S. Duarte, S. Seena, F. Cássio, C. Pascoal. Intraspecific diversity affects plant-litter decomposition in freshwaters. XVI Congress of the Iberian Association of Limnology, Guimarães, Portugal, July 2-6, 2012

7. S. Seena, S. Duarte, C. Pascoal, F. Cássio. An ubiquitous perspective of the intraspecific diversity of the aquatic fungus Articulospora tetracladia. XVI Congress of the Iberian Association of Limnology, Guimarães, Portugal, July 2-6, 2012

8. S. Seena, S. Duarte, C. Pascoal, F. Cássio. ITS barcodes as unambiguous discriminators of strains of Articulospora tetracladia. Poster presentation in the XIII Jornadas de Biologia Aplicada – A vida em revolução, Braga, Portugal, March 8-10, 2012.  

Theses

1. José Trabulo (2013). Effects of eutrophication on stream-dwelling decomposers of plant-litter. Master thesis in Ecology, University of Minho, Braga, Portugal.

2. Ilisa Antunes (2013). Effects of temperature and genetic diversity of aquatic hyphomycetes on leaf-litter decomposition. Graduation thesis in Applied Biology, University of Minho, Braga, Portugal (partially supported by FunDiver)

3. Mafalda Sousa (2013). Effects of diversity of aquatic hyphomycetes and eutrophication on leaf-litter decomposition in streams. Graduation thesis in Applied Biology, University of Minho, Braga, Portugal (partially supported by FunDiver)

4. Amaro Rodrigues (2012). Produção de documentários em vídeo para o ensino experimental da Ecologia. Master thesis in Ecology, University of Minho, Braga, Portugal (partially supported by FunDiver).

5. José Trabulo (2011). Does intraspecific identity of aquatic fungi affect plant-litter decomposition in streams under cadmium stress? Graduation thesis in Applied Biology, University of Minho, Braga, Portugal (partially supported by FunDiver). 

6. Bruno Antunes (2011). Effects of intraspecific diversity of aquatic fungi on plant-litter decomposition under cadmium stress. Graduation thesis in Applied Biology, University of Minho, Braga, Portugal (partially supported by FunDiver). 

DNA sequences

2015: Sequence Read Archives accession number SRR1958879 (31,478 ITS2 454 pyrosequencing reads).

2014: Sequence read archives accession number SRR1260517 (233,171 ITS2 454 pyrosequencing reads).

2013: 191 sequences of 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence (GenBank accession numbers KF730795 to KF730849 and KF952606 to KF952741).

2012: 112 sequences of 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence (JX089461 toJX089572).

2012: 41 sequences of 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence (JF895397 to JF895437).