ISHI 2017

October 1st, Seattle (USA)

Efficient Validation Using STR-validator

Validation of new STR typing kits are often time consuming which can delay implementation of new technology. STR-validator is a free open-source software tool designed to help forensic genetic laboratories evaluate the data. Lectures and demonstrations will exemplify experimental design and efficient evaluation. Examples will highlight quality control of the data and result. The goal is to introduce STR-validator as an efficient tool to analyze validation data in accordance to ENFSI recommendations/SWGDAM guidelines.

Learning Outcomes

  • Understanding of the STR-validator framework and its possibilities

  • Validation according to ENFSI recommendations/SWGDAM guidelines

  • Documentation and interpretation of the validation result

Intended Audience

This workshop is suitable for DNA analysts/technicians, QA/QC managers, consultants, and other interested individuals. A minimal background in forensic human identification testing would be beneficial. Programming or statistical background is not required.

Prepare for the workshop

  1. Download and install the statistical software R (usually installed together with a graphical user interface RGui or RStudio)

  2. Open RGui or RStudio and install the package strvalidator version 2.1.0 by typing install.packages("strvalidator", dependencies=TRUE) in the R console, or using the installation menu

  3. Download the zip file with exercises and unpack to a folder of your choice

  4. It is recommended that you follow the instructions in the installation guide to create an icon for STR-validator in your folder

Links

STR-validator

Blog post on Promega Connections

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