Biochemistry

PhD in Biochemistry, Rutgers, State University of New Jersey, New Brunswick, NJ.

Dissertation: Purification and characterization of the active N-terminal domain of mouse Tissue Inhibitor of Metalloproteinases-1, expressed in Escherichia coli.

Advisor: Prof. David T. Denhardt

Research Awards:

• Marion B. Johnson Fellowship, Rutgers University
• Nicholas C. Palczuk Research Fund, Rutgers University
• Anne B. and James H. Leathem Scholarship, Rutgers University
• Johanna and Charles Busch Memorial Fellowship, Rutgers University
• Merck Fellowship, Rutgers University

Peer-reviewed journal articles:

• Rajan, S. S., Lackland, H., Stein, S. and Denhardt, D. T. (1998) Presence of an N-terminal polyhistidine tag facilitates stable expression of an otherwise unstable N-terminal domain of mouse tissue inhibitor of metalloproteinase-1 in Escherichia coli. Protein Expr. Purif. 13(1):67-72.
• Rajan, S. S., Denhardt, D.T., Lackland, H. and Ludescher, R. D. (1998) Analysis of the conformational stability of the active domain of recombinant mouse TIMP-1 by intrinsic fluorescence. Biochem. Biophys. Res. Commun. 242(2):303-9.
• Denhardt, D. T., Rajan, S. and Walther, S. E. (1994) Structure-function studies of mouse tissue inhibitor of metalloproteinases-1. Ann. N. Y. Acad. Sci. 732:65-74.
• Cocuzzi, E. T., Walther, S. E., Rajan, S. and Denhardt, D. T. (1992) Expression and purification of mouse TIMP-1 from E. coli. FEBS Lett. 307(3):375-8.