Resolution

Sensitivity or in this case Resolution of the instrument is determined by the instrument's ability to resolve one dull fluorescent particle from a slightly brighter fluorescent particle. The Resolution Calculator allows you to calculate this. It follows the equation:where µ1 is the Mean of the duller population and µ2 is the Mean of the Brighter population. SD1 and SD2 are the standard deviations of the two populations. This gives a separation factor or resolution of your cytometer that is, it's used in calculating how well peaks will resolve in different setups e.g., different filter sets, or laser powers etc.,

Data Collection

Run one negative and one dull fluorescent beads and record their Means and Standard Deviations (SD). Enter the Bright beads mean and SD then enter the Dull mean and SD of the beads.

Results

The larger the S number the better resolved the peaks are from each other.