Worked as Research Associate (15.12.00-22.06.01) in Department of Biotechnology (DBT) sponsored project entitled “Decomposition of tea organic waste to value added compost” at Tocklai Experimental Station, Tea Research Association, Jorhat, Assam, India. The work was basically focused on enzyme assay and maintenance of pure cultures of identified bacterial and fungal strains.

Worked as Research Associate (8.09.01-22.05.04) in project “Identification of molecular marker linked to gene for submergence tolerance in rice” in Department of Biochemistry, Assam Agricultural University, Jorhat, India. This involved RAPD analysis and generation of SCAR primers, their validation by STRs and statistical analysis of the results obtained.

Worked as Senior Research Fellow(27.07.04-10.10.06) in Prof. Anil Grover’s lab and associated with DBT sponsored project “Identification of the salinity tolerance inducible genes in rice” in the Department of Plant Molecular Biology, University of Delhi, South Campus, India. This involved (1) Functional characterization of specific salt stress upregulated/downregulated (ESTs)/clones identified by using Reverse Northerns and conventional Northern hybridization and subsequent isolation and characterization of their promoters. Several promising salt stress associated genes like UBC4, eIF2α, PPiase, E3 Ubiquitin ligases etc have been used for the complementation of the corresponding yeast mutants. These genes have also been over expressed and under expressed in rice, yeast and tobacco to study their molecular mechanism in plants and to isolate the interacting proteins. (2) Analysis of two different heat shock protein pyramided rice plants and raising them to homogeneity. (3) Isolation & characterization of root specific promoter and cloning in binary vector pCambia1381Z with uidA gene in its downstream. The construct has been over expressed in rice plants and further analysis is under progress as it produces two transcripts under salt stress, a probable case of alternative splicing. (4) Analysis of over expressed tobacco basic 1,3 chitinase gene in Brassica plants by PCR, RT-PCR, southern, northern and western followed by Bioassay of the chitinase transgenics with Alternaria brassiceae to check its fungal resistance.

Worked as SRF (22.02.07-10.10-07) with Prof. R.C. Borah, Deptt. Of Biochemistry, AAU, Jorhat in a DBT sponsored project “Selection and Characterization of water logging resistant cultivars of tea”. It involves the conformation of the identified tea clones as water logging resistant by ADH assay and development of SSR marker to verify it at molecular level.

Worked as Post-Doctoral Scientist (Catalysis and Peptide Research Unit, UKZN, Durban) – Aug 2013 to Jan 2014. He secured 0.5 M RANDS Grant funding for this UKZN-PDF project in 2013. He successfully tackle the nagging problem of expressed proteins going into inclusion bodies by adjusting the culture parameters and even successfully harvesting inclusion bodies to rescue complete expressed HIV-1 protease. He formulated and successfully designed the scientific strategy while keeping in mind of learning focus of students to expressed and purify N15 labelled recombinant HIV-1 protease. The N15 labelled HIV-1 protease was purified and characterized by MALDI-TOFF and LC-MS for inhibitor development. He developed my skills of proteomics, protein structural characterization, enzyme assay and bioengineering techniques. He did GMO training under strict guidance of aseptic rules, ISO17025 and GMP guidelines. He also did some key gene expression alteration after HIV infection of the HPC was done. He could initiate the process to study the change in methylation patterns of the important genes for differentiation in to different type’s blood cells before leaving CPRU, Durban, RSA.

He worked as Post-Doctoral Scientist (Earth Institute, UCD, Dublin) – Jan 2014 to Dec 2018. He successfully completed all of the 3 projects within timelines.. Developed resource of 2016 GENOME EDITED validated lines of BRI1 receptor kinase for functional SNPs. He completed Phosphoproteomics project accomplished showing differential regulation of BRI1 signaling due to SNP in BRI1 kinase domain. He successfully validated the function of LRR-receptor kinase by reverse genetics approach, expression, signaling analysis, RNAi/VIGS suitable for patent application. Got patent (European patent Office application no.: EP17160440.8) for first wheat functionally characterized Fusarium resistance LRR receptor kinase. He disseminated project work and published papers in peer reviewed high impact factor journals. He completed assembled the NGS-HTS sequencing data, identified and validated functional SNPs. He managed team of diverse culture and background, solved technical issues, presented and establish collaboration to achieve goals, established for the first-time genome specific primer validation protocol, KASP and HRM assay in the lab, established strict GMO guidance of aseptic rules, ISO17025 and applicable GMP, introduced DNA samples Succession Planning in plates avoiding contamination and to avoid false positives, new inventory and lab Management System for quick access to chemicals and stocks.

He worked as Research Fellow (Health Research Institute, School of Biological Sciences, University of Limerick) – Jun 2019 to Ap 2020. He successfully generated protein hydrolysate from marine fish Blue Whiting that were found to alter the immune response of dendritic cells and Th cells in ex vivo culture. These hydrolysates showed potent anti-inflammatory activity, anti-diabetic and anti-allergic (tolerogenic properties) this may be of relevance to individuals suffering from chronic inflammation and inflammatory disorders. de novo novel potential anti-inflammatory peptides (AIPs) (3-21amino acids) were successfully identified and validated by machine algorithm and validated datasets. These AIPs were tested for immune response on immune human CD4/CD8, macrophages and THP-1 cells as well as look for anti-inflammatory activity especially nitric oxide signalling in mouse RAW264.7 cells. The immunogenic potential and tolerogenic effects were quantified in terms of key cytokines expression and levels at transcriptional, biochemical and translational levels. The THP-1 cells were successfully PMA-induced and differentiated in macrophages for successive immunoassays. Similarly, the human CD4/CD8 cells were successfully stimulated and differentiated using CD3 and CD28 Dynabeads or LPS into full potential proliferated CD4/CD8 cells for storage and immunoassays. He established key marine AIPs database, in silico pathway to identify AIPs, set up molecular and immune cell-based assay system here.

He worked as University Biosafety Officer & Biohazards Advisor (Trinity College of Dublin, Dublin)- June 2020. As University Biosafety Officer (Biological Hazards), he advised Trinity and oversees the broader safety management programme during Corona pandemic. He provided professional expertise in areas relating to the control of biological hazards across Trinity arising from work with all types of biological agents including genetically modified organisms and dangerous pathogens. He ensured the development of policies and procedures to meet with legislative requirements and the highest industry standards. He was accountable for promulgating these standards among the Trinity community. He coordinated with colleagues to provide provision of safety advice and safety services across a wide range of areas, in addition to the biological safety aspects of the work, to the University community. He also coordinated with colleagues to provide provision of safety advice and services across a wide range of areas, in addition to the biological safety aspects of the work, to the University community. He was active integral member of topmost governing body University biosafety committee (UBSC), University Containment level 3 laboratory (CL3) steering committee, Trinity Translational Medicine Institute (TTMI)/ Trinity Biomedical Sciences Institute (TBSI) CL3 Users Group, Strategic Oversight Committee and Chair of TCD CL3 Compliance Group. I was authorized with duty to review risk assessment, SOP and provide advices to PIs/researchers/students. As a Chair of the TCD CL3 compliance committee, He assured the recommended policy and advices was strictly followed. He was the module instructor of health and Safety course in TCD. He delivered CL3 training, modules and assessment apart from this module. This job came with utmost high responsibility and accountability to take care of whole research community of TCD post Corona pandemic.