@ University of Manchester
Welcome to the pages of the flow cytometry core facility within the Faculty of Biology, Medicine and Health at the University of Manchester.
Our facility offers researchers access to state-of-the-art flow cytometry analysis and sorting equipment, high parameter mass cytometry and imaging cytometry. The facility is managed full time by an experienced Senior Experimental Officer and is supported by two technical level staff.
We have available 6 flow cytometer analysers and 2 cell sorters. Additionally, we have a Helios-CyTOF for suspension mass cytometry, Hyperion Imaging Mass Cytometer and an ImageStreamX. Details of these instruments can be found in the links above.
Please contact Dr Gareth Howell (Facility Manager) for further information relating to accessing the facility.
Accessing the Facility
Register your account
We use Flowjo to perform third-party data analysis of the data produced in the Facility.
For more information and training resources on Flowjo please visit the website at http://flowjo.com
We have a number of Flowjo dongles that are available for loan from the Facility.
Please contact us for further information.
Workshops and Training
Please contact the Facility to discuss any panel design questions you may have.
We also run a short workshop 'Multicolour panel design' as part of the Technoshort series of workshops (detailed above).
To aid in panel design please read below about published optimized panels (OMIPs)
Flurochrome Brightness Index
Not all flurochromes are the same and one of the challenges of designing a multicolor flow cytometry experiment is balancng the anticipated expression level of the antign with the relaive brightness of the flurochrome. To aid this there are 'Brightness Index' tables where the relative brightness of common flurochromes are given.
Click here for the brightness index table (opens a new tab)
OMIPs (Optimized Panels)
For more information on OMIPs, please refer to the following articles:
Guidelines to use of the facility equipment
**NEW** Mandatory filtering of samples
Please remember to acknowledge the Core and our support grants in your papers if you accessed equipment, services, expertise, or data generated at the laboratory.
For detailed information on the funding source for the equipment click on the Equipment and systems page.
Suggested acknowledgement reference:
"We thanks the University of Manchester Flow Cytometry Core Facility for assistance with flow cytometry/ sorting/ discussions. The Flow Cytometry Core Facility is supported in part by the University of Manchester with assistance from <insert funding body>.