Colocalization Colormap
Colocalization studies are widely performed using methods based on global image analysis which for instance involves intensity correlation coefficients such as the Pearson correlation coefficient and the Manders overlap coefficient The general paradigm of these approaches is to plot the pixel values of two images against each other and display the result in a pixel distribution diagram called a scatter plot in a way in which an intensity of the given pixel in the first channel is used as the x-coordinate of the scatter plot and the intensity of the corresponding pixel in the second channel consists its y-coordinate. A linear, or non-linear relationship among the intensities of both channels might be then assumed. The disadvantage of methods based on general intensity correlation is lack of information about the spatial distribution of colocalized signals with respect to the composite nature of biological structures within the particular image. This website contains Colocalization Colormap ImageJ plugin that implements the Jaskolski's algorithm (Jaskolski et al. 2005). This innovative method produces a pseudo-color map of correlations between pairs of corresponding pixels in two original input images, thereby offering quantitative visualisation of colocalization.