生物質譜及轉譯蛋白體暨代謝體學研究室
Biological Mass Spectrometry & Translational Proteomic/Metabolomic Laboratory
生物質譜及轉譯蛋白質暨代謝體學研究室
(Dr. Yi-Ting Chen's Research Group)
長庚大學陳怡婷老師實驗室以生物質譜技術開發及分析化學為基礎，發展生物分子之分離技術與生物質譜檢測及定量方法；與泌尿科、腎臟科、婦產科、小兒科等臨床醫生合作，運用分析化學、生物質譜技術、蛋白質體及化學標定穩定同位素代謝體等後基因體學研究方法，全面性的研究正常與疾病生物系統(細胞株及臨床收集的體液或組織)中蛋白質與代謝物之群體變化；標靶體學的開發上，利用多重反應監測質譜儀(MRM-MS)以及穩定同位素化學標定方法，我們建立了超過兩百種生物或是疾病相關蛋白質/代謝小分子的標靶定量方法。臨床醫學應用方面，透過與國內及國外等機構，我們研究膀胱癌、腎臟癌、及腎衰竭病人的尿液、口腔癌症組織及口水、及婦產科羊水的蛋白質體學及代謝體學；並且在長庚大學的蛋白質體核心實驗室裡，負責標靶蛋白質質譜定量技術及化學標定穩定同位素代謝體技術平台的開發與維護，希望將實驗室的研發成果轉譯成為臨床醫生可以使用的診療工具，監測大小重要生物分子在疾病所扮演的角色，以期望朝向臨床應用及個人化醫療邁進。
Dr. Yi-Ting Chen's research focus is on developing high-throughput technologies to detection of biomolecules, primarily based on mass spectrometry for application in translational studies of urological diseases. Dr. Chen participates in several integrated research programs and serves as an investigator in the detection of bladder, kidney, and oral cancers using proteomics, metabolomics, and systems biology approaches to understand human diseases. Our group’s research is dedicated to the development of targeted and untargeted proteomic and metabolomic platforms for disease diagnosis / prognosis. We have established several workflows for the identification of novel biosignature candidate molecules, including proteins and metabolites, for human disease. The methods are primarily based on analytical chemistry, mass spectrometry and bioinformatics to study the real world and clinical unmet needs. We have established Omics approaches to investigate the alteration of biological molecules in various biomedical specimens including blood, tissue, cell lysates and secretome, urine, saliva, and amniotic fluids, etc. The current research focus is the development of a systems biology approach to the identification and quantification of proteins/peptides and metabolites associated with the bladder cancer, oral cancer, renal diseases, and energy-metabolism during disease progression. We have established the standard workflow for targeted and precise quantification of more than two hundred of biomolecules in a LC-MS run using multiple reaction monitoring mass spectrometry (MRM-MS), which has been called the gold standard of MS-based quantitation methods. The laboratory is interested in research directed toward disease biomarker discovery using clinical human tissues, body fluids and associated cell models. The laboratory is also involved in various collaborations with chemical and biomedical researchers as well as clinical physicians (oncologists, urologists, pediatrician, and obstetrician) at the national and international levels.
Internship programs (國際實習生招募中)
https://teep.studyintaiwan.org/program/616
ORCID: https://orcid.org/0000-0002-0063-4965
Lab website: https://sites.google.com/site/cguytc/home