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Analysis of food dye Brilliant Black in wine and caviar by liquid chromatography with concentration on chemically modified silica
The project was dedicated to investigation of preconcentration conditions of Food Dye E151 with following analysis by reverse phase high pressure liquid chromatography (RP HPLC) with photodiode detector.
In this regard, I have studied the adsorption and eluation behavior of the artificial dye E 151 and groups of artificial dyes (10 in total) on the modified silica sorbents with attached epoxy and Triton X-100 group. For the sake of comparison, commercially available "classical" sorbents with reverse phase C-16 and C-18 were additionally examined. The impact of 4 different for the composition and pH liquid phases on the analysis was evaluated. Measurements were carried out on 3 columns (RP C-18) with varied number of theoretical plates.
Model systems and real objects such as non alcoholic, alcoholic beverages and synthetic caviar were analyzed by developed approach.
Work was conducted under the supervision of Prof. V. M. Zaitsev and Dr. I. Pisareva. Silica sorbents with epoxy and Triton X-100 groups were kindly provided by Dr. V.Khalaf
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