ANIMAL MODELS

● Acute and chronic models of pharmacologically-induced epilepsy in mice and rats by systemic and brain injections of

pilocarpine, kainic acid, and pentylenetetrazol

EXVIVO MODELS

● Dispersed neuronal cultures

● Organotypic hippocampal cultures

IMAGING AND DETECTION TECHNIQUES

● Confocal and electron microscopy

● Immunohistochemistry techniques

● Immunogold techniques

● In situ hybridization

● In situ PCR

● In situ zymography

ELECTROPHYSIOLOGY

● Extracellular local field potential recordings in brain section

● Whole-cell patch clamp recordings in brain section

● EEG recordings

● Biocytine-based morphological reconstructions of cell morphology and projections

● Single cell electroporation

● In vivo electrode implantation

MOLECULAR BIOLOGY

● Basics of molecular cloning

● PCR and horizontal electrophoretic techniques

BIOCHEMISTRY

● 2D Electrophoretic techniques including western blotting, gel zymography

● Biochemical subcellular fractionation by differential centrifugation

● Co-immunoprecypitation

COMPUTER PROGRAMMING AND SOFTWARE DEVELOPMENT

● Colocalization Colormap –ImageJ plugin for quantification and visualization of colocalized fluorescent signals.

https://sites.google.com/site/colocalizationcolormap/home

● Matlab and Java programming in advanced image processing, morphometrics and signal analysis