Investigation Sources of Faecal Pollution and Microbial Risks in the River Kent

This project focuses on the upper River Kent, a nationally recognized Site of Special Scientific Interest (SSSI) and an international Special Area of Conservation (SAC) within the Lake District National Park, a UNESCO World Heritage Site. It aims to investigate the quantity and sources of faecal pollution and potential microbial risks using advanced biomolecular techniques. Specifically, the project will employ metagenomics and Microbial Source Tracking (MST) through ddPCR analysis to characterise the microbial composition and identify contamination sources. The findings from this project will be critical in safeguarding the ecological and public health of the River Kent. By understanding the sources and risks of microbial contamination, more effective measures can be implemented to reduce pollution and protect the river's natural environment. This project also has broader implications, offering a model for addressing similar issues in other river systems worldwide.