Penny E. Shockett, Ph.D.

RESEARCH INTERESTS:

(Molecular Immunology)

     In jawed vertebrates, adaptive immune responses are mediated by T and B lymphocytes. Displayed on the surface of these cells are highly diverse receptor proteins that allow specific recognition of the vast array of antigens that an organism normally encounters. Interestingly, during lymphocyte development, molecular events occurring at the DNA level help to generate antigen receptor diversity, but can also impact genomic stability and the development of lymphoid tumors. V(D)J recombination, the DNA recombination process that assembles the genes encoding the antigen binding portions of antigen receptors, cuts and pastes, mixing and matching individual gene segments to bring them together in different combinations to generate a variety of receptors. This developmental process requires a lymphocyte-specific DNA cutting nuclease consisting of RAG proteins, and ubiquitous DNA double strand-break repair proteins that help to reseal the breaks. Another DNA recombination process occurring in mature activated B cells specifies antibody effector function. My studies have generally helped to understand the inducers and biochemistry of these reactions during lymphocyte development and activation. I've also worked on creation of inducible transgenic mice for use in studying genes involved in these and other processes. Other areas of interest include NaV structure-function relationships, exercise immunology, and inflammation. Student expertise gained from working in my lab is generally in immunology, molecular biology, and biochemistry.

Ongoing Projects at Southeastern:

1.  Studies of timing and tissue specificity of aberrant and ectopic V(D)J rearrangements in mice potentially leading to genomic instability and tumor development. These studies were supported by R15 (AREA) funding from from the National Institute of Allergy and Infectious Disease of the National Institutes of Health.

2. Collaboration with Dr. John O'Reilly: Studies involve site-directed mutagenesis and structure-function analysis of human voltage-gated sodium channels (NaVs). This project has been funded by NIH Area Grants from the National Heart Lung and Blood Institute, and currently includes cloning  NaVs from nonhuman species for functional analysis.

3. Exercise and/or Inflammation: Studies in humans and mice aimed at identifying potential mechanistic links between beneficial health effects of exercise, inflammation, and the immune response. This includes mitochondrial DNA links to exercise (w/Dr. Robert Kramer), and recent interest in activities of neutrophil extracellular traps (NETs) across species (w/Dr. Chris Beachy and Dr. Pradip Panta).

EDUCATION:

1986-1993      Ph.D. Immunology,  University of Massachusetts Graduate School of Biomedical Sciences,
            Worcester, MA., Immunology Program, Dept. of Molecular Genetics and Microbiology.

                 Dissertation:  "Regulation of IgA class switch recombination in the I.29 mu B cell lymphoma
                                                       by cytokines and inhibitors of poly(ADP-ribose) polymerase."

1979-1983     A.B. Biology, Bowdoin College, Brunswick, ME.

POSITIONS HELD:

2016-Present  Professor, Department of Biological Sciences
                          Southeastern Louisiana University, Hammond, LA.

2006-2016  Associate Professor, Department of Biological Sciences
                          Southeastern Louisiana University, Hammond, LA.

2000-2006 Assistant Professor, Department of Biological Sciences
                          Southeastern Louisiana University, Hammond, LA.

1997-2000       Associate Research Scientist Section of Immunobiology,
                          Yale University School of Medicine, New Haven, CT.

1993-1997       Postdoctoral Fellow, Section of Immunobiology,
                          Yale University School of Medicine New Haven, CT.

1988-1993      Graduate Research Assistant Department of Molecular Genetics and Microbiology,
                         University of Massachusetts Graduate School of Biomedical Sciences, Worcester, MA.

1983-1986 Laboratory Technician in Histocompatibility Testing Lab
                        Massachusetts General Hospital, Boston, MA.

PROFESSIONAL EXPERIENCE:
2000-present   Southeastern Louisiana University, Hammond, LA., Department of Biological Sciences.

                          -Studies of developmental timing/tissue distribution of aberrant and ectopic V(D)J recombination events in vivo.

                          -Structure/function studies of Na+ channel slow inactivation by site-directed mutagenesis and cloning NaVs 

                                     from different species (w/John O'Reilly, Ph.D.)

                          -Studies of exercise links to immune system (w/Robert Kraemer) and NETs in different species (w/Chris Beachy & Pradip Panta)

1997-2000        Yale University School of Medicine, New Haven, CT., Section of Immunobiology.

                        -Molecular and biochemical studies of lymphocyte receptor variable region gene assembly in vitro. 

                           (w/David G. Schatz, Ph.D.)

1993-1997        Yale University School of Medicine, New Haven, CT., Section of Immunobiology.

                          -Development of system for inducible, tetracycline regulated gene expression in cultured cells and transgenic mice. 

                          -Application to studies of DNA recombination in developing lymphocytes. (w/ David G. Schatz, Ph.D.)

1988-1993     University of Massachusetts Graduate School of Biomedical Sciences, Worcester, 

                          Department of Molecular Genetics & Microbiology. 

                          -Studies of the regulation of immunoglobulin heavy chain class switch recombination by cytokines and 

                                      inhibitors of DNA repair. (w/Janet Stavnezer, Ph.D.)   

1983-1986         Massachusetts General Hospital, Boston, MA., Histocombatibility Testing Lab.

                           -Tissue matching for organ transplant and clinical immunology research.  (Director: Thomas C. Fuller, Ph.D.)

1982-1983   Bowdoin College, Brunswick, ME., Department of Biology.

                          -Angiogenisis in chick liver development.  (Honors project w/Glenn K. Sherer, Ph.D.)

COURSES TAUGHT:

2000-present     Southeastern Louisiana University:

MIC460/560        Immunology:  (4 credits, Every Fall) Upper level undergraduate/graduate lecture with laboratory. Text: 7th Ed. Kuby Immunology                                                                                                                                                                          

MIC463/563        Virology :  (4 credits, Every Spring until 2019) Upper level undergraduate/graduate lecture w/seminar component.  Text: Voyles,                                                 The Biology of Viruses, 2nd Edition, Shores, Understanding Viruses 3rd edition

MIC 434/534       Molecular Biology & Biotechnology:  (4 credits, Developed Spring 2019). Upper level undergraduate/graduate lecture with                                                                                                  laboratory.

GBIO200            Cell Biology:   (3 credits, Every Fall and Spring)(Undergrad). Text: Albert's et al., Essential Cell Biology: An Introduction to the                                                     Molecular Biology of the Cell. 5th edition

GBIO 690          Advanced Immunology.  (1 credit, Spring 2011) Graduate Seminar course involving readings/discussion relating                                                                                                           to epigenetic regulation of gene rearrangement in the immune system.

GBIO 690   Advanced Eukaryotic Molecular Genetics (1 credit, Spring 2025) Graduate Seminar course involving readings/discussion relating                                                                                     to current advanced topics in eukaryotic molecular genetics. 

GBIO441            Biology Seminar:  (1 credit, Fall and Spring, several previously). Majors capstone course in general and research-focused areas                                                 of biology and presentation methods in biological sciences.  Text: Purves et al., Life: the Science of Biology, 6th Ed.

GBIO341            Professional Aspects of Biology:  (1 credit, Fall 2005). Majors course involving student attendance at seminars presented by                                                                                       individuals in professional fields related to biology.

BIOL152            General Biology Laboratory I:  (1 credit).  

TA Mentor for: Catherine Bechtold (Fall 2000), Jessica Mayfield (Fall 2004, Spring 2005),

Jessica Chancey (Fall 2005, Spring 2006), Adronisha Frazier, (Fall 2010, Spring 2011), 

Brianna Myre (Spring 2014), Heather Guillot-Gautreaux (Spring 2014), Corey Samples (Fall 2021), 

Madelyn Pye (Fall 2023, Spring 2024),Madeline Denman (Fall 2024), Logan Jarrell (Spring 2025)

Teaching Honors:  

2005                 Received student nomination for and was chosen for Who's Who Among American Teachers. 

2018                 Southeastern Louisiana University-Outstanding Teacher Award-Gamme Beta Phi Honor Society

STUDENTS:

Student Research Trainees (and subsequent positions):

-UNDERGRADUATES:

Carl Gautier, pre-med, OSCAR grant recipient, biology major (Spring 2001-Spring 2002) (LSU Medical School)

Dwayne Delrie, pre-med, chemistry major (Summer 2001-Spring 2002) (Tulane Medical School)

Jason Eleser, OSCAR grant recipient, pre-med., biology major (Summer 2002-Spring 2003) (LSU Medical School)

Amy Melancon, biology major, (Spring 2003), assisted Erica Perrer (Medical Technology Certification Program)

Alicia Manuel, biology major (Fall 2003), assisted Catherine diBenedetto (SLU Graduate Program in Biology)

Daniel King, biology major (Fall 2003, Spring 2004), assisted Charles Nebel (Private Environmental Testing Lab)

Jessica Sprague (Mayfield), biology major (Spring 2004) (SLU Graduate Program in Biology)

Chadney Glover, biology major (Summer 04), assisted Jessica Mayfield (SLU Graduate Program in Biology)

Jessica Chancey, OSCAR grant recipient, biology major (Fall 04, Spring 05) (SLU Graduate Program in Biology)

                               (University of Alabama at Birmingham, Cellular & Molecular Biology Ph.D. Program, Ph.D. Candidate)

                               (Post Doctoral Fellowship, NIH)(Post Doc, U.T. Austin)(Assistant Professor, U.T. Austin, Neuroscience)

Elisha Barnes, pre-med, biology major, assisted Jessica Klopf (Medical School Applicant)

Margaret Stire, biology major (Fall 05)

Lanna Kuehler, biology major (Spring 06, collab. w/ Dr. John O'Reilly)

Wendy Boysha, biology major (Spring 06)

Jameka Kemp, biology major (Summer 06, collab. w/Dr. John O'Reilly)

Charlie Golmon.biology major, STAR grant recipient (Spring 07, Summer 07, Fall 07, Spring 08 (Private Environmental Testing Co.)

Mindy Guidry, pre-med, biology major (Fall 2007) (Medical School Applicant)

Greg Landry, biology major (Spring 2008)  

                  (LSU Health Sciences Center, Shreveport, Ph.D. Program Dept. of Pharmacology Toxicology, and  Neuroscience)

                (Post Doctoral Fellowship, Mayo Clinic, MN) (Associate Professor, Massachusetts College of Pharmacy and 

                   Health Sciences)                                                                                        

Christie Duvernay. biology major, (Fall 2008) (LSU Medical Technologist Program)

Janice Fan pre-optometry, biology major (Fall 2008) (University of Memphis College of Optometry)

Melissa Eddy, pre-med, biology major (Fall 2008) (Pre-Med Masters Program, Mississippi College)(LSU Medical School)

Meggie Doucet, pre-med, biology major, STAR grant recipient (Fall 2008-Spring 2009)(LSU Medical School, Shreveport)

Carolyn Rubin, pre-Vet/Ph.D., (Summer 2009) (LSU College of Veterinary Medicine & Ph.D. Program)

Adronisha Frazier, biology major, honors thesis,  (Fall 2009-Spring 2010) (SLU Graduate Program in Biology)(LSU, Ph.D, Sci. Ed)

Jeremy Stagray, biology major, honors thesis (Spring 2010, collab. w/Dr. Robert Kraemer)

Bradley Bursavich, biology major, (Spring 2010, collab. w/Dr. Robert Kraemer) (LSU Medical School)

Dana Mayer, pre-med, biology major (Spring 2010, collab. w/Dr. Robert Kraemer)  (Medical School, Canada)

Devin Champagne, chemistry major, collab. w/Dr. Robert Kraemer) (SLU Graduate Program in Biology)

Tricia Adams, biology major, (Fall 2010)  (LSU Department of Animal Sciences Ph.D. Program)

David Ward, pre-med, biology major (Spring/Sum 2010,collab. w/Dr. John O'Reilly) (M.D./Ph.D. Program-LSU Medical School)

Pramir K.C., biology major, (Spring 2011-Spring 2013)(Graduate M.S. Program in Biology, Valdosta State University, GA) 

                                                                                       (PhD Program U. of Halifax)

Jacob Delaune, biology major (Spring 2012, collab. w/Dr. Robert Kraemer)(LSU Graduate Program in Education)
Heather Robin, biology major (Spring 2012, collab. w/Dr. Robert Kraemer)

Alina Situala, biology major (Spring 2013, Spring/Fall 2014, Spring 2015, collab. w/Dr. Robert Kraemer and Dr. John O'Reilly

            (Research Associate, National Jewish Research Institute, Denver Co.), (M.S. Genetic Counseling-Univ. Minnesota)

Amy Weaver, biology major, (Spring 2013)

Van Ziebarth, biology major (Spring 2013) (Medical School Applicant)

Januka Khanal, biology major, (Fall 2013,-Spr/Sum/Fall 2014, Spring 2015), SURE (LA EPSCoR) Grant Recipient, collab. w/Dr. Robert Kraemer. 

                             (National Institute for Mathematical and Biological Synthesis Summer Research Experience for            

                                   Undergraduates and Teachers Program (SRE)-Univ. of Knoxville, TN., Summer 2015)

                             (Research Associate, Dana Farber Cancer Institute, 2016-2018).  

                             (Ph.D. Program-Molecular Biophysics and Biochemistry-Yale University)

Ross Weidemann, biology major, Summer 2014, (LSU Medical School)

Jacob Stagray, biology major, honors thesis, Summer/Fall 2014, Spring 2015

                              (LSU Shreveport, Graduate Biomedical Ph.D. Program in Immunology and Microbiology)
                              (University of Louisiana at Lafayette, Graduate Ph.D Program in Biology)(Post Doc, Yale Neurobiology)
Lillia Lara-Sanchez, pre med, volunteer Spring/Summer/Fall 2016, Spring/Summer 2017

Kevin Bokun, biology major, pre med, honors thesis, Fall 2016/Spring 2017, collab w/Dr. John O'Reilly) (LSU Medical School, Shreveport)

Binisha Karki, biology major (Spring/Fall 2018), Research Assistant (Sloan Kettering, NY, NY.), (Research Associate BioNTech, Boston, MA.)
Dalton Cambre, biology major (Spring 2018)
Alexander Melton, biology major (Fall 2018)
Lauren Frazier, biology major, (Fall 2018,)
Ali LeBourgeois, biology major, Honors Thesis Student (Fall 2018/Spring 2019)
Pragya Khadka. biology major, (Fall 2018)
Lauryn Scott, biology major, (Spring 2021)

Amy Schneida, biology major (Fall 2021, collab w/Dr. John O'Reilly)(M.S. Program in Applied Psychology, University of New Orleans)
Maiah Woodring, biology major, (Fall 2021, Spring 2022), (Graduate Ph.D. Program in Chemistry (Biochemistry)-Baylor University)

Pranup Adhikari, biology major, (Fall 2022, Spring 2023)

Dahlia Alkadi, biology major, (Spring 2023)

Harley Garlik. biology major, (Spring 2024)

Fajr Fayed, biology major (Summer 2024)

Morgan Brown, biology major (Summer 2024)

Sara Ramos DeLong, biology major (Summer 2024)

-GRADUATE STUDENTS:

Catherine diBenedetto-graduate student in biology  (Masters Thesis Defended Jan 2004)

(Research Associate II, MannKind Corp. (Diabetes and Cancer Research), Drug Discovery Department, Valencia, Ca.)        

(2007-present. Senior Scientist, Assay Development & Compound Profiling, Center for Integrative Chemical Biology

            and  Drug Discovery, University of North Carolina, Chapel Hill)

Charles Nebel, graduate student in biology (Non-Thesis Masters Degree, Dec. 2005)

             (SLU and Delgato Community College part-time Instructor, Volunteer in Shockett Lab (Spring 2005)

Erica Perrer, graduate student in biology, SLU graduate teaching scholarship recipient (Non-Thesis Masters Degree, Spring 2005)

             (Our Lady of the Lake College, Microbiol. Lab Coord. & Instructor 

             (Delgato Community College, Instructor)                                                                                                  

Jessica Mayfield, graduate student in biology

             (Non-Thesis Masters Degree, Spring 2006)(Instructor, SLU Biology Dept.)

Jessica Klopf, graduate student in biology (Non-Thesis Masters Degree, Spring 2006)

              (Associate Scientist, Reliagene Technologies, Metarie LA.)

              (Medical Research Technician, Dept. of Pharmacology, Tulane Health Sciences Center)

              (LA. Teacher Certification)

Rebecca Wallace, (Non-Thesis Masters Degree, Spring 2007). Volunteered (Fall 2004) and worked in lab for graduate course credit (Spring 2005).

Jessica Chancey, graduate student in biology, SLU Graduate School Fellowship Recipient 

             (Masters Thesis Defended, April 2008) (Co-advisor, Dr. John O'Reilly)

             (University of Alabama at Birmingham, Cellular & Molecular Biology Ph.D. Program, Ph.D. Candidate)

             (Post Doctoral Fellowship, NIH)(Post Doc, U.T. Austin)(Assistant professor, U.T. Austin Neuroscience)

Wallissa Lancelin, (Spring-Fall 2009)(Non-Thesis Masters Degree, Fall 2009)

             (Research Associate, Pennington Biomedical Research Institute)(Houston School of Optometry)

Nancy Dardis (Spring 2010) (collaboration with Robert Kraemer) (Masters Thesis Defended, Nov. 2011)

             (Cardiac Rehabilitation Specialist)

Devin Champagne-graduate student in biology,

             (Masters Thesis Defended, May 2013)

             (University of VT., Molecular, Cellular, and Biomedical Sciences Ph.D. Program)(Post Doc. U. Colorado Denver)

              (Assay Development Scientist-Genocea) (Medical Sales Manager-Medical Diagnostic Laboratories, Medical Sales Manager -Bruker)

Adronisha Frazier- graduate student in biology

             (Non-Thesis Masters, Dec. 2013) (University of Houston, Microbiology Laboratory Technician)(Instructor-Introductory Biology,

              Houston Community College)(Instructor North Shore Community College, LA.) (Graduate Ph.D. Student-Science Ed., LSU)

Jonathan Beard- graduate student in biology (Co-advisor, with Dr. John O'Reilly)-(Masters Thesis defended May 2019).                                                              (Ph.D Program, Baylor University, Cell, Molecular, Health, and Disease Tract.)

Leandrew Pate III-(Summer/Fall 2023)(Non-Thesis Masters Degree, Spring 2024)

Madeline Pye- graduate student in biology (Masters Thesis Defended, June 2025)

GRANTS and HONORS:

2024   SGA Departmental Grant, Supplies for Molecular Biology & Biotechnology and Immunology Courses, $1500. 

2023   Center for Faculty Excellence Enhancement Grant, "Neutrophil Extracellular Traps in Mice and Salamanders", $750.

2018-2021      Duane and Catherine Shafer Endowed Professorship in Biological Sciences ($7500/year for three years).     

2015-2016      Center for Faculty Excellence Enhancement Grant, "Testing methods for tracking cell-free mitochondrial
        DNA in exercising mice".  ($700)

2014-2015      LA EPSCoR (NSF) Supervised Undergraduate Research Experience (SURE) Grant, for Januka Khanal.

        "Modulation of  Mitochondrial DNA with Exercise". ($4000 student stipend, $500 expenses).

2013-2014      Center for Faculty Excellence Enhancement Grant, "Modulation of Mitochondrial DNA with Exercise" ($698).

2013     American Association of Immunologists Undergraduate Faculty Travel Grant, to attend AAI Annual Meeting            

                       "Immunology 2013, Honolulu, Hawaii ($1500 and $1000 for Undergrad. Student)

2010-2013       NIH R15 Grant, National Institute for Allergy and Infectious Disease,
        "Spatiotemporal Comparison of Aberrant and Ectopic V(D)J Recombination Events in Vivo" ($195,195).

2009-2010 CONDUITS Grant, Southeastern Office of Research and Graduate Studies. (Collaborator with Robert Kraemer) ($2000)

2009-2010 Teaching Enhancement Grant-Southeastern Faculty Excellence ($500)

2009-2010 Student Technology Fee-Southeastern Office of Technology, (Collaborator with Robert Kraemer ) ($4,133.60)

2008         CONDUITS Grant.  $535.00 Supported purchase/shipment of mouse grain-based diet containing tetracycline.

2008-2011      NIH AREA Grant, National Heart Lung and Blood Institute, "Role of Segment 6 in Heart Na Channel Slow
                        Inactivation", Competitive Renewal, ($186,895 for three years) (Collaborator with J. O'Reilly)

2007-2008      LEAD Grant, Southeastern Louisiana University, "Proteomics at Southeastern and Beyond", ($15,288).

2007-2008      Faculty Development Grant, Southeastern Louisiana University,
                        "Inducible RAG Transgenic Mice: A Tool for Analysis of Constraints on V(D)J Rearrangement
                        in Primary Lymphocytes and Nonlymphoid Cells. I. A comparison of cellular signals that activate
                        V(D)J accessibility and rearrangement in fetal vs. adult nonlymphoid cells."
        (Continuation Request, $1,984 for 1 year)

2007               Southeastern Louisiana University Student Technology Fee Small Project Proposal:
        "New Sensor Board and Motor for the Molecular Immunology Laboratory Incubator" ($1,853)

2006       Southeastern Louisiana University Student Technology Fee Small Project Proposal:
                        "Technology-based teaching and learning in biology"
                        (J. Bossart, F. Campo, P. Shockett, R. Valverde) ($5,000)

2006-2007      Faculty Development Grant, Southeastern Louisiana University, "Validation of 2D Protein Gel  

                       Analysis of DNAPKcs Phosphorylation Targets During V(D)J Recombination" ($980)

2005-2008      NIH AREA Grant, National Heart Lung and Blood Institute, "Role of Segment 6 in Heart Na Channel Slow

                       Inactivation", ($179,092 for three years) (Collaborator with J. O'Reilly)

2005               Southeastern Louisiana University Student Technology Fee Small Project Proposal:"Molecular Biology 

                       Technology for Neurobiology and Immunology Laboratories" (w/J. O'Reilly) ($4,432.40)

2005               Southeastern Louisiana University Student Technology Fee Small Project Proposal:

          "Enhanced Technology for Neurobiology and Immunology Laboratories" (w/J. O'Reilly) ($2,523)

2005               Southeastern Louisiana University Student Technology Fee Surplus Computer Proposal:

                       For For 2 Pentium III computers with monitors and printers.

2005-2006      Faculty Development Grant, Southeastern Louisiana University, "Inducible RAG Transgenic Mice:

        A Tool for Analysis of constraints on V(D)J Rearrangement in Primary Lymphocytes and Nonlymphoid

        Cells. I. A comparison of cellular signals that activate V(D)J accessibility and rearrangement in fetal vs. adult

                        nonlymphoid cells." (Continuation Request, $1,310 for 1 year)

2004               SLU Student Technology Fee Small Project Proposal, "A white light conversion screen for visualization and

                       documentation of proteins on polyacrylamide gels using the BioRad ChemiDoc system."  ($400)

2004               SLU Student Technology Fee Surplus Computer Proposal: For 2 Pentium III computers with monitors.

2004-2005       Faculty Development Grant, Southeastern Louisiana University, "Inducible RAG Transgenic Mice: 

        A Tool for Analysis of Constraints on V(D)J Rearrangement in Primary Lymphocytes and Nonlymphoid

        Cells.  I.  A comparison of cellular signals that activate V(D)J accessibility and rearrangement in fetal vs. adult

        nonlymphoid cells. ($1,800).

2004-2006      EPA Western Lake Pontchartrain Basin Research Program,  "Are Polycyclic Aromatic Hydrocarbons

        Stressors for Lymphocyte Development or Activation in Frog Populations in Bayou Trepagnier?" ($100,000)

2002               Student Technology Fee Grant, Southeastern Louisiana University, "Biotechnology equipment for the new

                       Biological Sciences classroom and laboratory building",  (w/C. Jackson) ($24,188).

2001               SLU Student Technology Fee Grant, "A Peltier Thermocycler for the Department of Biological Sciences"

                       (P. Shockett, G.Howard, G. Childers, and C. Jackson) ($4,842).

2001-2004      Louisiana Board of Regents Support Fund Grant, Research Competitiveness Subprogram. "Role of DNA-

                       dependent protein kinase catalytic subunit in coding end processing during V(D)J recombination."  ($92,805 for 3 years)

2001-2002.     Faculty Development Grant, Southeastern Louisiana University

                       "Diversity of receptors in the immune system: Role of the DNA-dependent protein kinase catalytic subunit" ($1,960 for 1 year).

1995-1998 Postdoctoral Fellowship, Arthritis Foundation ($80,500 for 3 years)

1993-1995 Competitive Postdoctoral Traineeship, NIH Training Grant, Section of Immunobiology,  Yale University School of Medicine

1979-1983 Magna Cum Laude in Biology, Student Advisor to the Biology Department,  Bowdoin College, Brunswick, ME.

PUBLICATIONS:

(* denotes student author)

Beard, J. *, Shockett, P., and O'Reilly, J. (2020). Substituted cysteine scanning in D1-S6 of the sodium channel hNav1.4 alters kinetics and structural interactions of slow inactivation. BBA-Biomembranes 1862 (2):183129.

Shockett, P. , Khanal, J.*, Sitaula, A.*, Oglesby C. *, Meachum, W., Castracane, V.D., Kraemer, R. (2016) Plasma Cell-Free Mitochondrial DNA Declines in Response to Prolonged Moderate Aerobic Exercise. Physiological Reports, Jan 4(1) e12672 Pub Med

Champagne, D.P.* and Shockett, P.E. (2014). Illegitimate V(D)J Recombination-mediated deletions in Notch1 and Bcl11b are not sufficient for extensive clonal expansion and show minimal age or sex bias in frequency or junctional processing. Mutation Research 761:34-48.  Pub Med

Kraemer, R.R., Shockett, P., Webb, N.D.*, Shah, U., and Castracane, V.D. (2014). A Transient Elevated Irisin Blood
Concentration in Response to Prolonged, Moderate Aerobic Exercise in Young Men and Women. Horm Metab Res. Feb;46(2):150-4. Pub Med

O'Reilly, J.P., and Shockett, P.E. (2012) Time- and state-dependent effects of methanethiosulfonate ethylammonium (MTSEA) exposure differ between heart and skeletal muscle voltage-gated Na+ channels. Biochimica et Biophysica Acta-Biomembranes March 1818(3):443-447. Pub Med

Chancey, J.H.*, Shockett, P.E., and O'Reilly, J.P. (2007).  Relative Resistance to Slow Inactivation of Human Cardiac Na+ Channel hNav1.5 is Reversed by Lysine or Glutamine Substitution at V930 in D2-S6. American Journal of Physiology: Cell Physiology Pub Med

O'Reilly, J.P. and Shockett, P.E. Conformational Change in Segment 6 of Domain 2 in  Human Cardiac Na+ Channel hNav1.5 During Slow Inactivation (2006).  Biochem Biophys Res Commun  345:59-66. Pub Med

Shockett, P. and Schatz, D. (2005). UNIT 20.8: Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System, in Current Protocols in Cell Biology John Wiley & Sons, Inc.

Shockett, P., Zhou, S., Hong, X. and Schatz, D. (2004). Partial reconstitution of V(D)J rearrangement and lymphocyte development in RAG-deficient mice expressing inducible, tetracycline-regulated RAG transgenes.  Molecular Immunology 40: 813-829.  Pub Med

Chen, J., Kelz, M., Zeng, G., Steffan, C., Shockett, P, Terwilliger, G., Schatz, D., and Nestler, E. (2002).  Inducible, Reversible Hair Loss in Transgenic Mice. Transgenic Research 11:241-247.  Pub Med

#Fugmann, S. D., Lee, A. I., Shockett, P. E., Villey, I.J., and Schatz, D.G. (2000).  The RAG Proteins and V(D)J Recombination: Complexes, Ends, and Transposition. Annual Review of Immunology, 18: April 2000.  Pub Med
(#The first four authors are listed in alphabetical order and contributed equally to this work)

Shockett, P. E. and Schatz, D. G.  (1999).  DNA hairpin opening mediated by the RAG1 and RAG2 proteins.  Molecular and Cellular Biology.  Vol 19, 4159-4166.  Pub Med

Chen, J., Kelz, M., Zeng G., Sakai N., Steffen C., Shockett, P., Picciotto M., Duman, R., and Nestler, E. (1998).  Transgenic animals with inducible, targeted gene expression in the brain.  Molec. Pharm. 54, 495-503. Pub Med

Shockett, P. and Schatz, D. (1998).  Inducible expression in mammalian cells using a tetracycline-regulated system.  In, Cells: A Laboratory Manual, Volume 1, Section 5, Systems for Protein Expression.  Cold Spring Harbor Laboratory Press.

Shockett, P. and Schatz, D. (1997).  Inducible gene expression using an autoregulatory, tetracycline-controlled system.  Current Protocols in Molecular Biology.  Unit 16.2.1

Shockett, P. and Schatz, D. (1997). Switching on gene expression.  Nature Biotechnology 15, 221-223. .

Shockett, P. and Schatz, D. (1996).  Diverse strategies for tetracycline-regulated inducible gene expression.  PNAS, USA 93, 5173-5176.  Pub Med

Shockett, P., Difilippantonio, M., Hellman, N.* and Schatz, D. (1995).   A modified  tetracycline-regulated system provides autoregulatory, inducible gene expression in  cultured cells and transgenic mice.  PNAS, USA 92, 6522-6526. Pub Med

Shockett, P. and Stavnezer, J. (1993).  Inhibitors of poly(ADP-ribose) polymerase increase antibody class switching.  J. Immunol.  151, 6962-6976. Pub Med

Lin, Y. Shockett, P. and Stavnezer, J. (1992).  Regulation of transcription of the germline immunoglobulin alpha constant region gene.  Curr. Top. Microbiol. Immunol. 182, 157-165.

Shockett, P. and Stavnezer, J. (1991).  Effect of cytokines on switching to IgA and alpha-germline transcripts in the B lymphoma I.29 mu:  Transforming growth factor-beta activates transcription of the unrearranged C-alpha gene.  J. Immunol. 147, 4374-4383.   Pub Med

Lin, Y. Shockett, P. and Stavnezer, J. (1991).  Regulation of the antibody class switch to IgA.  Immunol. Res. 10:376-380.

Stavnezer, J., Shockett, P., Lin, Y. Saikh, K. and Xu, M. (1990).  The I.29 mouse B cell lymphoma:  Regulation of the antibody class switch. In Cytokines. Basel, Karger, vol 3, 154-169.

PRESENTATIONS:

Pye, M., Pate III, L., Panta, P., Beachy, C.K., and Shockett, P.  (2024). Studies of Neutrophil Extracellular Traps in Mice and Other Species. American Society for Microbiology - Fall Conference Joint Meeting of KY/TN ASM and South Central Branch ASM, Memphis TN.

Pate III, L., Pye, M., Panta P., Beachy, C.K., and Shockett, P. (2024). NETS in Mice (Now) and Salamanders (Soon).

Louisiana Academy of Sciences 98th Annual Meeting, Hammond, LA.

Pate III, L., Pye, M., Panta P., Beachy, C.K., and Shockett, P. (2024). NETS in Mice (Now) and Salamanders (Soon).

22nd Louisiana Biomedical Research Network Annual Meeting, Baton Rouge, LA.

Beard, J., Shockett, P., and O'Reilly, J. (2019). Using Scam to Investigate Reconfiguration of Molecular Determinants in D1-S6 during Slow Inactivationof hNav1.4. Annual Meeting of the Biophysical Society, Baltimore, MD.

O'Reilly, J., Bokun, K., Beard, J., and Shockett, P. (2019). In Search of a Molecular Mechanism for Slow Inactivation in Voltage-Gated Na Channels using the SCAM Technique in D2-S6 of hNav1.4. Annual Meeting of the Biophysical Society, Baltimore, MD.

O'Reilly, J. and Shockett, P. (2017). Effects of Cysteine Substitutions in D1-S6 on Fast and Slow Inactivation in Nav1.4. Annual Meeting of the Biophysical Society, New Orleans, LA.

Shockett, P. (2016). Why Immunity and Pathogens are our Frienemies. Science on Tap, Biological Seminar Series for the Public. Downtown Hammond, Louisiana. Feb 2016  

Shockett, P. (2015). Cell-free Mitochondrial DNA and Exercise. University of Mississippi, Biology Seminar Series.

Shockett, P. , Khanal, J.*, Sitaula, A.*, Meachum, W., Castracane, V.D., Kraemer, R. (2015). Decline in Relative Plasma Cell-Free Mitochondrial DNA Levels in Response to Prolonged Moderate Aerobic and Eccentric Exercise. American Association of Immunologists Annual Meeting, New Orleans, LA.

Khanal, J.*, Sitaula, A.*, Shockett, P., Kraemer, R. (2015). Decline in Relative Plasma Cell-Free Mitochondrial DNA Levels in Response to Prolonged Moderate Aerobic and Eccentric Exercise. Louisiana Academy of Sciences 89th Annual Meeting, Nicholls State University, Thibodeaux, LA.

Kraemer, R.R. Shockett,  P.E., Dardis Webb, N.*, Shah, U., Castracane, V.D. (2014). A Transient Elevated Irisin Blood Concentration in Response to Prolonged, Moderate Aerobic Exercise in Young Men and Women. American College of Sports Medicine Annual Meeting, Orlando Florida.

Champagne, D.* and Shockett P. (2013).  Analysis of Illegitimate Notch1 and Bcl11b V(D)J Deletion Events During Mouse Development. American Association of Immunologists Annual Meeting, Honolulu, Hawaii.

Champagne, D.* and Shockett, P. (2013). Analysis of illegitimate V(D)J recombination events in Notch1 and Bcl11b during mouse development.  Louisiana Academy of Sciences, 87th Annual Meeting, Grambling State University.

Champagne, D.* and Shockett P. (2012).  Spatiotemporal analysis of illegitimate V(D)J deletions in Notch1 and Bcl11b genes. American Association of Immunologists Annual Meeting, Boston, MA.

Champagne, D. and Shockett, P. (2012). Spatiotemporal Analysis of Illegitimate V(D)J Deletions in Notch1 and Bcl11b Genes.  Louisiana Academy of Sciences, 86th Annual Meeting, LSU Alexandria.

O'Reilly JP, Shockett PE, Chancey JW* (2009).  Structure-function analysis of slow inactivation in voltage-gated Na+ channels.  Louisiana Academy of Sciences Annual Meeting, Southeastern Louisiana University.

Chancey, J, Shockett, P., and O'Reilly, J. (2007).  Lysine substitution at V930 in D2-S6 of hNav1.5 greatly enhances slow inactivation.  Annual Meeting of the Biophysical Society, Baltimore, Maryland.

Shockett, P. (2004). Molecular Mechanisms Generating Lymphocyte Antigen Receptors,  Southeastern Louisiana University Biology Seminar Series.

Shockett, P. (2004). Molecular Mechanisms Generating Lymphocyte Antigen Receptors,  University of Southern Mississippi, Biology Seminar Series.

Shockett, P. (1999).  Mechanisms of Assembly of Lymphocyte Antigen Receptors. Southeastern Louisiana University, Dept. of Biological Sciences.

Shockett, P. (1999). Mechanisms of Assembly of Lymphocyte Antigen Receptors. Lafayette College, Dept. of Biology, Easton, PA.

Shockett, P. (1999). Lymphocyte Development and Gene Rearrangements In Vivo and In Vitro. Genzyme Transgenics, Inc. Framingham, MA.

Shockett, P. (1999). Lymphocyte Development and Gene Rearrangements In Vivo and In Vitro. Nextran Corp., Princeton, NJ.

Shockett, P. and Schatz, D, (1999).  RAG-mediated opening of hairpin coding ends in vitro to generate P regions.  Workshop on V(D)J Recombination.  Keystone Symposium on B Lymphocyte Biology and Disease. Taos, New Mexico.

Shockett, P. and Schatz, D. (1999). DNA hairpin opening mediated by the RAG1 and RAG2 proteins. Yale University Section of Immunobiology Retreat. June 1999. (and '98,'97,'96,'95,'94-various titles and authors)

Shockett, P. and Schatz, D. (1996).  Construction of transgenic mice inducibly expressing the RAG genes. Keystone Symposium on Lymphocyte Activation.  Hilton Head, South Carolina.

Shockett, P. and Schatz, D. (1995).  Construction of transgenic mice inducibly expressing the RAG genes. Genetic Recombination & Genome Rearrangements.  FASEB Summer Conference, Snowmass, Colorado.

Shockett, P. (1995). Characterization of a tetracycline-regulated system for inducible transgenic mice. The Dupont Merck Pharmaceutical Co.,  Experimental Station, Wilmington, Delaware.

Shockett, P. and Stavnezer, J. (1991).  Regulation of germline-α transcripts and switching in the I.29μ B-cell lymphoma. FASEB summer research conference, Saxtons River,Vermont.

Lin, Y., Shockett, P. and Stavnezer, J. (1990).  Regulation of Immunoglobulin α-germline transcripts prior to heavy chain switch in I.29μ cells.  New England Immunology Conference, Woods Hole Ma.

Stavnezer, J., Shockett, P. and Severinson, E. (1989).  Effect of lymphokines on expression of RNAs transcribed from germline immunoglobulin heavy chain constant region genes in the I.29 B cell lymphoma. 7th International Congress of Immunology, Berlin.

Shockett, P. and Woodland, B. (1988).  A unique B cell subpopulation in diabetes-prone BB/W  rats, absent in diabetes resistant sublines.  UCLA Symposia on Molecular and Cellular Biology, Symposium on B Cell Development (J. Cell  Biochem. Supp12B).

Yearly Departmental Seminars:

1988-1992, Immunology & Virology Group, Univ. of Massachusetts, Grad. School of Biomed. Sci.

1994-1999. Section of Immunobiology, Yale Univ. School of Medicine.