The Efficacy of Genipin Crosslinking of Decellularized Porcine Articular Cartilage to Enhance Resistance to Biochemical Degradation
Courtesy of https://www.maxpixel.net/Plants-Fragrance-Nature-Petal-Gardenia-Fragrant-3520319
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Hello! My name is Tvishi Yendamuri and I am an AP Research student at Rock Canyon High School. For my research, I will be examining and assessing the efficacy of genipin, a natural, low cell toxic agent derived from the Gardenia fruit (as seen in the header picture), for crosslinking with decellularized porcine articular cartilage. Decellularization is the process of chemically removing the cellular material of living tissues, creating an acellular extracellular matrix (ECM). The subsequent step of reseeding is to seed the ECM with a patient's own stem cells, thus reducing and even eliminating tissue rejection. However, decellularization harms the structure of the ECM (which needs to be "strong" for seeding cells), so it is important to find a way to strengthen the ECM after decellularization. Thus, I will be looking at building up the ECM's proteins through genipin crosslinking. This research will contribute to the limited conversation and literature on the feasibility of using engineered cartilage as non-immunogenic replacement tissue and the potential crosslinking process with genipin.
zoom_0 copy.mp4You can watch my research proposal pitch right here!
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