Watch this Video 1st
How to use a Microscope
Watch this video 2nd
How NOT to use a Microscope
Make sure to TAKE NOTES on what TO DO and NOT TO DO with our microscopes in your microscope manual. You will be quizzed on this before you use a microscope in class.
Parts of a Microscope
Do you know the parts of the Microscope?
Take this Quiz to test your knowledge.
1. Plug in the microscope and turn on the lamp.
2. Place the Low Power Objective Lens (the shortest) facing towards the stage
3. Place the slide on the stage and secure in place with stage clips.
4. Use the Coarse Adjustment Knob (large knob) to bring the slide into view then use the Fine Adjustment Knob (small knob) to make it clearer.
5. Once you have the image in view, rotate the nosepiece to view it under different magnifications.
**Be careful if you want to use the highest power objective lens (the long one). If the stage is too close it might not fit and therefore scratch the lens!**
6. When done:
turn off the lamp
place low power objective facing down
put the slide away
wrap the cord
cover the microscope
Make sure the cord is wrapped around the arm (and the end is tucked in) and that the low power objective is facing down.
Place one hand on the arm, and the other hand on the base.
Lift up and walk carefully.
Multiply the power of the eyepiece lens by the power of the objective lens. Our microscopes have an eyepiece lens magnification of 10x.
Low Power:
10x (eyepiece lens) x 4x (low power objective)
= 40 times magnification
Medium Power:
10x (eyepiece lens) x 10x (medium power objective)
= 100 times magnification
High Power:
10x (eyepiece lens) x 40x (high power objective)
= 400 times magnification
Use this to study for the Microscope Safety Quiz
Use this interactive to learn how to use the microscopes we will use in class. https://www.knowitall.org/interactives/hobbyshop/Microscope/index.html
Use this interactive to learn how to use the microscopes we will use in class.
Use this interactive to learn how to use the microscopes we will use in class.
How to make a Wet Mount Slide
How to make a wet mount:
•Cut a lowercase letter. preferably and e, from the newspaper
•Place in the centre of a clean slide
•Put a drop of water on the top of the letter using an eyedropper
•If too much water is added, the cover slip will “float” creating a water
layer that is too thick
•If too little water is added, the specimen may be crushed or dry out too
quickly
•Place the edge of a cover slip against the water and with a pencil gently
lower the cover slip over the letter
•Placing the cover slip in the manner prevents air bubbles from forming
underneath the cover slip