Syllabus (Syllabus is available in google classroom)

TEKS: select subchapter O in STEM: https://goo.gl/UVMzdh

1^st 6 weeks:

Lab Safety, Equipment, and Common Lab Techniques

Introduction to the Scientific Method and Statistics

• Greatest Discoveries

Lab: Statistics in the lab/Calibration and Validation of Micropipettes

mini-Lab: Preparing Biological Assays, Quality, and SOPs

Quantitative and Qualitative Tests

Lab: Gram Stain / Optical Microscope/The Kirby-Bauer Antibiotic Sensitivity Test

Lab: Titration of aspirin and spectrophotometry

Lab: Quantitative Analysis of Proteins by the Bradford Assay

2^nd 6 weeks:

Investigative and Experimental Design

Lab: Protein Expression-BioBuilder

Lab: Water Quality in the Biotech Lab

Lab: Chromogenic Analysis of Water Contaminants

Lab: Pyrogen Assay of Water/Microplate Reader

Lab: Mitochondrial DNA by PCR

3^rd 6 weeks

Introduction to Research

Lab: Cloning and Sequencing/GAPDH gene

Extraction, Amplification, Ligation, Transformation

mini-preps, and bioinformatics

Final Fall Exam and Project Presentations

4^th 6 weeks

Design of Experiments

Lab: Protein Profiler and Western Blot

5^th 6 weeks

Data Analysis and Organization Skills

Lab: Fish DNA barcoding and Sequencing

Review for BACE exam (Biotechnology Certification)

6^th 6weeks

Showcase Event and Project Presentation

Topics on Stem Cells and CRISPR/Cas9

Research topics in cancer

Final Spring Project Exam

*Field Trips and work collaboration: to be discussed