Laboratory of Histology and Embryology

Head of the laboratory

Assistant Professors

PhD students

Technical Staff

inż. Andrzej Leszczyszyn

Katarzyna Jackowiak

Tel. +48 61 848 7627

Research

I. Anatomy, histology and morphogenesis of the organs of the digestive system in vertebrates (reptiles, birds and mammals) with histodifferentiation of the structures of the mucosa.

(Tasks: Morphofunctional and comparative analysis of the structure of tongue and palate with taste organ in selected feeding groups of mammals and birds; Ultrastructural and molecular characteristics of para- and ortokeratinization processes in the oral cavity in domestic and wild-living birds and mammals; Ultrastructure of the gallbladder and biliary tract in vertebrates)

II. Anatomy, histology and morphogenesis of the organs of the reproductive system and urinary system

(Tasks: Spatial 3D reconstruction and topography of the genital and urinary organs in pre- and postnatal period in female cats; Morphogenesis of the ovary, fallopian tube and the uterus)

III. Angioarchitecture of the organs of the digestive and reproductive systems with special emphasis to the process of angiogenesis

(Tasks: Spatial arrangement and development of vascular system in the selected organs of the digestive and genital tract in mammalian species; Ultrastructural and immunohistochemical analysis of the development of capillary networks).

Methods and techniques used in research

I. Light microscopy (LM):

- Preparation of histological slides (paraffin and resin method)

- Topographic, histochemical and immunohistochemical methods. Morphometric analysis of histological slides

- Methods of three-dimensional analysis (3D imaging) of microscopic structures

- Raman spectroscopy

II. Scanning and transmission electron microscopy (SEM, TEM):

- Methods of preparation of biological and non-biological samples

Maceration techniques

III. Microcorrosion casting - methods of preparation of vascular corrosion casts (VCC) of the blood vessels networks for the three-dimensional visualization of the angioarchitecture on the examined organs in the scanning electron microscope.

Cooperation

In the country :

- UAM - Poznań (Adam Mickiewicz University in Poznań)

- Politechnika Poznańska (Poznań University of Technology)

- Uniwersytet Warmińsko-Mazurski – Olsztyn (University of Warmia and Mazury in Olsztyn)

- Uniwersytet Śląski - Katowice (Silesia University in Katowice)

International:

- Belgium, Ghent University, Faculty of Veterinary Medicine, Department of Morphology

- UK, University of Nottingham, School of Veterinary Medicine and Science, Faculty of Medicine & Health Sciences

Publications

Jackowiak H., Skieresz-Szewczyk K., Kwieciński Z., Godynicki S, Jackowiak K., Leszczyszyn A. 2015. Light and SEM studies on the reduction of the tongue microstructures in the White Stork (Ciconia ciconia, Aves). Acta Zoologica 96, 436-44, doi.org/10.1111/azo.12087

Prozorowska E., Jackowiak H. 2015. Light Microscopy and Scanning Electron Microscopy Study on Microstructure of Gallbladder Mucosa in Pig. Microscopy Research and Technique, 78: 220-229. https://doi.org/10.1002/jemt.22465

Skieresz-Szewczyk K., Jackowiak H. 2016. Morphofunctional study of the tongue in the domestic duck (Anas platyrhynchos f. domestica, Anatidae); LM and SEM study. Zoomorphology. 135, 255-268. doi: 10.1007/s00435-016-0302-2

Kociucka B. Jackowiak H. Kamyczek M., Szydłowski M., Szczerbal I. 2016: The relationship between adipocyte size and the transcript levels of SNAP23, BSCL2 and COPA genes in pigs Meat Science 121 – 12-18. DOI: 10.1016/j.meatsci.2016.05.011

Skieresz-Szewczyk K., Jackowiak H. 2017. Development of mechanical papillae of the tongue in the domestic goose (Anser anser f. domestica) during the embryonic period. Protoplasma 254, 147-160. doi.org/10.1007/s00709-015-0927-x

Jackowiak H., Skubis J., Łakomy P., Nasiadka P. Godynicki S. 2017. Functional morphology of the tongue and lingual papillae in the fallow deer (Dama dama, Linnaeus, 1758). Mammalian Biology 85, 14-23. doi.org/10.1016/j.mambio.2017.02.003

Skieresz- Szewczyk K., Jackowiak H., Buchwald T., Szybowicz M. 2017, Localisation of alpha-keratin and beta-keratin (corneous beta protein) in the epithelium on ventral surface of the lingual apex and its lingual nail in the domestic goose (Anser anser f. domestica) by using immunohistochemistry (IHC) and Raman microspectroscopy analysis. Anatomical Record 300, 1361-1368 . DOI: 10.1002/ar.23591

Jackowiak H. , Jerbi H., Skieresz-Szewczyk K., Prozorowska E. 2017. LM and SEM Studies on Tongue and Lingual Papillae in the Donkey (Equus asinus) Chapter in book: Microscopy and imaging science: practical approaches to applied research and education, Microscopy books series. Publisher: Formatex, Editors: A. Méndez-Vilas, Ed, pp.216-222. ISBN13 978-84-942134-9-6.

Stachowiak M., Szczerbal I., Nowacka- Woszuk J., Jackowiak H., Sledziński Iskrzak P., Dzimira S, Świtoński M. 2017. Polymorphisms in the SOX9 region and testicular disorder of sex development (38,XX; SRY-negative) in pigs. Livestock Science 203, 48-53. doi.org/10.1016/ j.livsci.2017.07.002

Skieresz-Szewczyk K., Jackowiak H., Ratajczak M. 2018. Ultrastructural study on the embryonic development of the orthokeratinized epithelium and its cornified layer (lingual nail) on the ventral surface of the lingual apex in the domestic goose (Anser anser f. domestica). Zoology 126: 36-45. doi.org/10.1007/s00709-018-1324-z

Skieresz-Szewczyk K., Cornille P., Jackowiak H 2018: The development of lingual glands in the domestic duck (Anas platyrhynchos f. domestica) - 3D reconstruction, LM and SEM study. Journal of Morphology. 279:319–329. 10.1002/jmor.20774

Prozorowska E., Jackowiak H. 2018: Morphology and topography of internal reproductive organs in the female cat during prenatal and postnatal development; SEM and 3D reconstruction study. Journal of Morphology 279 :1764-1775. 10.1002/jmor.20895

Prozorowska E. Jackowiak H. Ratajczak M. 2019: Ultrastructural study of uterine epithelium in the domestic cat during prenatal development. Theriogeneology 130: 49-61. 10.1016/j.theriogenology.2019.02.035

Skieresz-Szewczyk. Jackowiak H., Ratajczak M. 2019: Embryonic development of parakeratinized epithelium of the tongue In the domestic duck (Anas platyrhynchos f. domestica): LM, SEM and TEM observations. Protoplasma 256, 631–642. doi.org/10.1007/s00709-018-1324-z

Skieresz-Szewczyk K., Jackowiak H. 2019: Alfa and beta-keratin in the parakeratinized epithelium of the tongue in the domestic goose (Anser anser f. domestica). J. Experimental Zoology - B - Molecular and Developmental Evolution 332: 158-166. 10.1002/jez.b.22892

Franke-Radowiecka A., Prozorowska E., Załęcki M., Jackowiak H., Kaleczyc J. 2019: Innervation of the female internal genital organs in the pig during prenatal development. J. of Anatomy 235: 1007-1017. doi.org/10.1111/joa.13052

Kubale, V., Prozorowska, E., Glocová, K., Slater, L., Rutland, C. S. 2020. The Function of Seven Transmembrane Receptors in the Cardiovascular System and Their Role in the Development of Cardiomyopathy. Chapter in book: In Cardiac Diseases. IntechOpen. DOI:10.5772/intechopen.92628

Plewa B., Jackowiak H. 2020. Macro- and microscopic study of the tongue of Bison bonasus hybrid as an interspecific species (Bos taurus × Bison bonasus). Microscpy Research Technique, 83, 1241-1250. doi.org/10.1002/jemt.23516

ProJECTS

Projects in progress

Statuatory project: “Microscopic morphology of organs of farm and wild animals”. Project manager Prof. H.Jackowiak, Contractors: Dr K.Skieresz- Szewczyk, dr E. Prozorowska, MSc Barbara Plewa

Research financed within the project: “Regional Initiative of Excellence - RID” in 2019-2022

2020 - “Analysis of the growth factors regulating the process of angiogenesis during the development of the intramural vascular networks in the uterus of the domestic cat in the pre- and postnatal period”. Project manager: Dr Ewelina Prozorowska.

2019 - “Histomorphometric analysis of the uterine wall during the postnatal development of the domestic cat (Felis silvestris catus)”. Project manager: Dr Ewelina Prozorowska.

Completed projects:

NCN/KBN/EU projects and other

2019-2020 „Analysis of the gap junctions in the ortho- and parakeratinized epithelium of the mucosa of the organs of the bird's beak cavity". Project manager: Dr Kinga Skieresz-Szewczyk. MINIATURA no. NCN 2019/03/X/NZ4/01246.

2010-2013 - “EUROPEAN MASTER IN COMPARATIVE MORPHOLOGY” – Grant EU. Project number – 510132-LLP-1-2010-1-BE-ERASMUS-ECDCE (2010-2013). Project manager: Dr hab. Hanna Jackowiak

2010 – „Osteoarthritis of the hip joints (coxarthrosis) in terms of changes in the chemical composition and spatial configuration of collagen of the examined bone tissue of patients in orthopedic hospitals in the city of Poznań”Grant in cooperation of Poznań Life Sciences University. Medical university in Poznań, Adam Mickiewicz University and Technical University in Poznań Project contractor: Dr hab. Hanna Jackowiak

2007 – „Microstructure of capillary network sin mucosa of gallbladder and raccoon dog (Canidae, Carnivora)”. Grant in cooperation of Poznań Life Sciences University and Adam Mickiewicz University, Project manager: Dr hab. Hanna Jackowiak

Faculty research grants for young scientists

2019 - “Differentiation of blood vessels in the uterine wall of domestic cat (Felis silvestis catus) in the prenatal period”. Project manager: Dr Ewelina Prozorowska.

2018 - “Identification of the process of angiogenesis in the uterine wall of the domestic cat (Felis silvestris catus) during the prenatal development”. Project manager: Dr Ewelina Prozorowska.

2018 - “Analysis of the distribution of the intercellular junctions in the ortho- and parakeratinized epithelium of the tongue in adult birds during embryonic development”. Project manager: Dr Kinga Skieresz-Szewczyk. No. 507.511.02.

2017 - “Biochemical analysis of cytokeratins and other proteins involved in the keratinization of the oral mucosa epithelium in birds”. Project manager: Dr Kinga Skieresz-Szewczyk. No. 507.511.2.

2016 - “Analysis of the keratinization of the epithelium of the lingual mucosa in birds”. Project manager: Dr Kinga Skieresz-Szewczyk. No. 507.511.62.

2013 - “Developmental analysis of the vascular system in the reproductive organs in female domestic cat (Felis silvestris catus)”. Project manager: prof. dr hab. Hanna Jackowiak. Contractor: Ewelina Prozorowska, MSc. No. 507.511.45.

2012 - “Development of the structure of the reproductive organs in the domestic cat (Felis silvestris catus) in the pre- and postnatal period”. Project manager: prof. dr hab. Hanna Jackowiak. Contractor: Ewelina Prozorowska, MSc. No. 507. 511. 18.

2010 - 2011 - “Development of the lingual mucosa in Anseriformes from 9^th till 25^th day of incubation”. Project manager: Dr hab. Hanna Jackowiak. Contractor: Kinga Skieresz-Szewczyk, MSc. No. 418/Z/511/W.

2010 – 2011 - „Development of mucosa of tongue in domestic cat in prenatal period (Felis silvestris catus) during prenatal period.” Project manager: dr hab. Hanna Jackowiak, contractor . MSc Mgr Joanna Trzcielińska. : 419/Z/511/W

TeaCHING

Course: Biology - Bachelor degree

- Histology of animals

- Embryology of animals

- Developmental Biology

Biology - Master degree - Experimental biology/Plants Biology/Animals Biology

- Microscopic techniques

Applied Biology (since academic year 2019/2020) - Bachelor degree

- Histology of vertebrates

- Embryology of vertebrates

- Biology of vertebrates development

- Biology of human development with teratology

- Biology of sensory organs

Veterinary Medicine

- Histology and embryology of animlas part I and II

- Organogenesis

- Microscopic techniques

Course: Zootechnics/Animal science - Bachelor degree

- Virtual microscopy of tissue and selected organs of farm animals

- Outline of animal embryology with teratology

Zootechnics/ Animal science - Master degree - Animal Nutrition

- Anatomy and histology of digestive system

Zootechnics/ Animal science - Master degree – Breeding and Animal Genetics

- Embryology of animals

Animal Nutrition – dual studies, Master degree

- Anatomy, histology and regulation of the digestive system

Animal Production Management, Master degree

- Embryology within Module 10: Preventive Veterinary Medicine

Course: Biotechnology - engineering /bachelor degree

- Embryology of animals

Virtual Microscope