Shoji M, Hashimoto R, Nebigil CG, Takegawa K, Tomita I, Nakaoka K, Ishikawa M, Matsumura Y, Nagao M, Esumi T, Takahashi E, Kido H, Shinohara Y, Désaubry L, Takayama K, Kuzuhara T. Host-directed antiviral strategy targeting prohibitins: Mel56 suppresses influenza A virus and severe acute respiratory syndrome coronavirus 2 via modulation of antioxidant pathways and mitochondrial function. Microbiology Spectrum. e03093-25. 2026.
https://doi.org/10.1128/spectrum.03093-25 journals.asm.org/doi/10.1128/spectrum.03093-25
Hatakeyama, D., Shoji, M., Kuzuhara, T. (2026). Pharmacophores of Influenza Virus PA Endonuclease Inhibitors. In: Suzuki, Y., Prinetti, A., Sriwilaijaroen, N., Wu, A.M. (eds) The Molecular Immunology of Complex Carbohydrates - 4. Advances in Experimental Medicine and Biology, vol 1491. Springer, Cham. https://doi.org/10.1007/978-3-032-04153-1_25
Hatakeyama D, Shoji M, Miki Y, Ueki S, Yamaguchi K, Nakashima K, Tanaka Y, Yokogawa M, Osawa M, Kasai Y, Imagawa H, Hashimoto R, Takayama K, Kuzuhara T. Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics. Sci Rep 16, 12769 (2026). https://doi.org/10.1038/s41598-026-42404-3 www.nature.com/articles/s41598-026-42404-3
Shoji M, Nakaoka K, Ishikawa M, Kasai Y, Esumi T, Takahashi E, Kido H, Imagawa H, Kuzuhara T. Triethylamine inhibits influenza A virus infection and growth via mechanisms independent of viral neuraminidase and RNA-dependent RNA polymerase. PLoS One., 20(8), e0329964 (2025). journals.plos.org/plosone/article?id=10.1371/journal.pone.0329964
Shoji M, Ohashi T, Nagase S, Yuri H, Ichihashi K, Takagishi T, Nagata Y, Nomura Y, Fukunaka A, Kenjou S, Miyake H, Hara T, Yoshigai E, Fujitani Y, Sakurai H, Dos Santos HG, Fukada T, Kuzuhara T. Possible involvement of zinc transporter ZIP13 in myogenic differentiation. Sci. Rep., 14(1), 8052 (2024). www.nature.com/articles/s41598-024-56912-7
Shoji M, Esumi T, Masuda T, Tanaka N, Okamoto R, Sato H, Watanabe M, Takahashi E, Kido H, Ohtsuki S, Kuzuhara T. Bakuchiol targets mitochondrial proteins, prohibitins and voltage-dependent anion channels: New insights into developing antiviral agents. J. Biol. Chem., 300(2), 105632 (2024).
Hatakeyama D, Tanii H, Nishikawa E, Takahira M, Honjo T, Ebisuda N, Abe N, Shinohara Y, Mitomo S, Tsutsui A, Fujita T, Kuzuhara T. Anacardic Acid Derivatives Isolated from Fungal Species Tyromyces fissilis as New Histone Acetyltransferase Inhibitors. Biol Pharm Bull., 47(12), 2076-2082 (2024).
Shoji M, Ichihashi K, Sriwilaijaroen N, Mayumi H, Morikane S, Takahashi E, Kido H, Suzuki Y, Takeda K, Kuzuhara T. Anti-influenza Activity of Povidone-Iodine-Integrated Materials. Biol. Pharm. Bull., 46, 1231-1239 (2023).
Shoji M, Okamoto R, Unno T, K, Kubo M, Fukuyama Y, Kuzuhara T. Transcriptome Analysis of PC12 Cells Reveals That trans-Banglene Upregulates RT1-CE1 and Downregulates abca1 in the Neurotrophic Pathway. Biol Pharm Bull., 45, 1784-1790 (2022).
Hatakeyama D, Sunada H, Totani Y, Watanabe T, Felletár I, Fitchett A, Eravci M, Anagnostopoulou A, Miki R, Okada A, Abe N, Kuzuhara T, Kemenes I, Ito E, Kemenes G. Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS. FASEB J. 36, e22593 (2022).
Hatakeyama D, Shoji M, Ogata S, Masuda T, Nakano M, Komatsu T, Saitoh A, Makiyama K, Tsuneishi H, Miyatake A, Takahira M, Nishikawa E, Ohkubo A, Noda T, Kawaoka Y, Ohtsuki S, Kuzuhara T. Acetylation of the influenza A virus polymerase subunit PA in the N-terminal domain positively regulates its endonuclease activity. FEBS J., 289, 231-245 (2021).
Hatakeyama D, Masuda T, Miki R, Ohtsuki S, Kuzuhara T. In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5. Biochem Biophys Res Commun., 557, 273-279 (2021).
Shoji M, Sugimoto M, Matsuno K, Fujita Y, Mii T, Ayaki S, Takeuchi M, Yamaji S, Tanaka N, Takahashi E, Noda T, Kido H, Tokuyama T, Tokuyama T, Tokuyama T, Kuzuhara T. A novel aqueous extract from rice fermented with Aspergillus oryzae and Saccharomyces cerevisiae possesses an anti-influenza A virus activity. PLoS One, 16, e0244885. (2021).
Shoji M, Esumi T, Tanaka N, Takeuchi M, Yamaji S, Watanabe M, Takahashi E, Kido H, Yamamoto M, Kuzuhara T. Organic synthesis and anti-influenza A virus activity of cyclobakuchiols A, B, C, and D. PLoS One, 16, e0248960. (2021).
畠山大、庄司正樹、葛原隆. インフルエンザウイルスRNAポリメラーゼが有するエンドヌクレアーゼ活性を阻害する化合物群 BIO Clinica, 35(2), 73-77, (2020).
葛原隆. 生命科学の最前線 精子や卵子はもういらない？ー幹細胞からの個体の構築へー. 都薬雑誌, 42(1), 20-24, (2020).
Shoji, M, Ueda, M, Nishioka, M, Minato, H, Seki, M, Harada, K, Kubo, M, Fukuyama, Y, Suzuki, Y, Aoyama, E, Takigawa, M, Kuzuhara, T. Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells. Biochemical and Biophysical Research Communications, 519(2), 309-315, (2019).
Hatakeyama D, Shoji M, Yamayoshi S, Yoh R, Ohmi N, Takenaka S, Saitoh A, Arakaki Y, Masuda A, Komatsu T, Nagano R, Nakano M, Noda T, Kawaoka Y, Kuzuhara T. Influenza A virus nucleoprotein is acetylated by histone acetyltransferases PCAF and GCN5. J. Biol. Chem., 293(19), 7126 –7138 (2018).
Shoji M, Minato H, Ogaki S, Seki M , Suzuki Y, Kume S, Kuzuhara T. Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells. PLoS ONE, 13(7), e0201239, (2018).
Kukula-Koch W, Koch W, Czernicka L, Głowniak K, Asakawa Y, Umeyama A, Marzec Z, Kuzuhara T. MAO-A Inhibitory Potential of Terpene Constituents from Ginger Rhizomes—A Bioactivity Guided Fractionation. Molecules, 2018, 23, 1301; doi:10.3390/molecules23061301, 2018.
Hatakeyama D, Ohmi N, Saitoh A, Makiyama K, Morioka M, Okazaki H, Kuzuhara T. Acetylation of lysine residues in the recombinant nucleoprotein and VP40 matrix protein of Zaire Ebolavirus by eukaryotic histone acetyltransferases. Biochemical and Biophysical Research Communications, 504(4), 635-640 (2018).
Shoji M, Woo SY, Masuda A, Win NN, Ngwe H, Takahashi E, Kido H, Morita H, Ito T, Kuzuhara T. Anti-influenza virus activity of extracts from the stems of Jatropha multifida Linn. collected in Myanmar. BMC Complementary and Alternative Medicine, doi: 10.1186/s12906-017-1612-8, 2017.
Yusuke S. Kato, Masaru Tanokura, Takashi Kuzuhara. NMR spectra of PB2 627, the RNA-binding domain in influenza A virus RNA polymerase that contains the pathogenicity factor lysine 627, and improvement of the spectra by small osmolytes. Biochemistry and Biophysics Reports , 12, 129–134, 2017.
Shoji M, Nishioka M, Minato H, Harada K, Kubo M, Fukuyama Y, Kuzuhara T. Neurotrophic activity of jiadifenolide on neuronal precursor cells derived from human induced pluripotent stem cells. Biochemical and Biophysical Research Communications, 470 (4), 19 February 2016, 798–803.
Shirayama R, Shoji M, Sriwilaijaroen N, Hiramatsu H, Suzuki Y, Kuzuhara T. Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines. Drug Discoveries & Therapeutics. 2016; 10(2):109-113.
Shoji M, Arakaki Y, Esumi T, Kohnomi S, Yamamoto C, Suzuki Y, Takahashi E, Konishi S, Kido H, Kuzuhara T. Bakuchiol is a Phenolic Isoprenoid with Novel Enantiomer-Selective Anti-Influenza A Virus Activity Involving Nrf2 Activation. J Biol Chem., 290(46), 28001-28017, 2015
Hiasa M, Kurokawa M, Akita H, Harada M, Niki K, Ohta K, Shoji M, Echigo N, Kuzuhara T. Suppression of increased blood glucose levels in mice by Awa-ban tea following oral administration of mono- and disaccharides. Journal of Functional Foods, 8, 188–192, 2014
Hatakeyama D, Shoji M, Yamayoshi S, Hirota T, Nagae M, Yanagisawa S, Nakano M, Ohmi N, Noda T, Kawaoka Y, Kuzuhara T. A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication. J. Biol. Chem. 2014 Sep 5; 289(36): 24980-24994.
Hiasa M, Isoda Y, Kishimoto Y, Saitoh K, Kimura Y, Kanai M, Shibasaki M, Hatakeyama D, Kirino Y, Kuzuhara T. Inhibition of monoamine oxidase A and stimulation of behavioural activities in mice by the inactive prodrug form of the anti-influenza agent oseltamivir. British Journal of Pharmacology, 169, 115–129, 2013
Shoji M, Takahashi E, Hatakeyama D, Iwai Y, Morita Y, Shirayama R, Echigo N, Kido H, Nakamura S, Mashino T, Okutani T, Kuzuhara T. Anti-influenza activity of C60 fullerene derivatives. PLoS ONE 8(6): e66337, 2013.
Hiasa M, Kurokawa M, Ohta K, Esumi T, Akita H, Niki K, Yagi Y, Echigo N, Hatakeyama D, Kuzuhara T. Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea. Food Research International, 54 (2013) 72-80
Hatakeyama D, Tierling S, Kuzuhara T, Müller U. Chapter 10. Epigenetic regulation of gene expression in the nervous system. Methods in Neuroethological Research. Eds. Ogawa, H, Oka K. Springer. 2013. pp. 151-171.
葛原隆、核酸の代謝・DNAの合成、pp. 183-225, 薬学領域の生化学（第２版）伊藤晃編集, 廣川書店. 2013.
Tsurumura T, Qiu H, Yoshida T, Tsumori Y, Hatakeyama D, Kuzuhara T, Tsuge H. Conformational Polymorphism of m(7)GTP in Crystal Structure of the PB2 Middle Domain from Human Influenza A Virus. PLoS One. 8(11): e82020, 2013.
葛原隆 インフルエンザRNAポリメラーゼ 病原性の強さと種間の伝播に関与する立体構造とエンドヌクレアーゼを阻害する化合物群 ファルマシア (2012) 48(6), 499-503.
Kuzuhara T, Tsuge H. インフルエンザウイルスRNAポリメラーゼのドメインの立体構造 The tertiary structures of the domains of influenza RNA polymerase Seikagaku. 2012 Sep;84(9):780-5.
Iwai Y, Murakami K, Gomi Y, Hashimoto T, Asakawa Y, Okuno Y, Ishikawa T, Hatakeyama D, Echigo N, Kuzuhara T. Anti-Influenza Activity of Marchantins, Macrocyclic Bisbibenzyls Contained in Liverworts. PLoS ONE (2011) 6(5), e19825.
Ludwiczuk A, Saha A, Kuzuhara T, Asakawa Y. Bioactivity guided isolation of anticancer constituents from leaves of Alnus sieboldiana (Betulaceae). Phytomedicine (2011) 18(6): 491-498.
Saha A., Kuzuhara T., Echigo N., Fujii A., Suganuma M., Fujiki H. Apoptosis of human lung cancer cells by curcumin mediated through up-regulation of growth arrest and DNA damage inducible genes 45 and 153. Biol. Pharm. Bull. (2010) 33(8): 1291-1299.
Saha A., Kuzuhara T. Echigo N., Suganuma M., Fujiki H. New Role of (-)-Epicatechin in Enhancing the Induction of Growth Inhibition and Apoptosis in Human Lung Cancer Cells by Curcumin. Cancer Prevention Research (2010) 3(8): 953-962.
Kimura Y., Yamatsugu K., Kanai M., Echigo N., Kuzuhara T., Shibasaki M. Design and synthesis of resin-conjugated Tamiflu analogs for affinity chromatography. Bull. Korean Chem. Soc. (2010) 31: 588-594.
Iwai Y, Takahashi H, Hatakeyama D, Motoshima K, Ishikawa M, Sugita K, Hashimoto Y, Harada Y, Itamura S, Odagiri T, Tashiro M, Sei Y, Yamaguchi K, Kuzuhara T. Anti-influenza Activity of Phenethylphenylphthalimide Analogs Derived from Thalidomide. Bioorganic & Medicinal Chemistry (2010) 18, 5379-5390.
Kuzuhara T., Iwai Y., Takahashi H., Hatakeyama D., Echigo N. Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase. PLoS Currents Influenza. (2009) Oct 13: RRN1052.
Tsuge H., Tsurumura T., Utsunomiya H., Kise D., Kuzuhara T., Watanabe T., Fujiki H., Suganuma M. Structural basis for the Helicobacter pylori-carcinogenic TNF-alpha-inducing protein. Biochem. Biophys. Res. Commun. (2009) 388:193-8.
Kimura Y., Tamatsugu K., Kanai M., Echigo N., Kuzuhara T., Shibasaki M. Design and synthesis of immobilized Tamiflu analog on resin for affinity chromatography. Tetrahedron Lett. (2009) 50: 3205-3208. (50th anniversary issue)
Kuzuhara T., Kise D., Yoshida H., Horita T., Murazaki Y., Utsunomiya H., Tsuge H. Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2. Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. (2009) 65:144-6.
Kuzuhara T., Kise D., Yoshida H., Horita T., Murazaki Y., Nishimura A., Echigo N., Utsunomiya H., Tsuge H. Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity-determinant lysine 627 residue. J. Biol. Chem. (2009) 284: 6855-60.
Kuzuhara T., Kise D., Shirakawa Y., Sasada K., Suganuma M., Fujiki H. Generation of mouse monoclonal antibody against (-)-epigallocatechin gallate. Biol. Pharm. Bull. (2008) 31:816-9.
Suganuma M., Yamaguchi K., Ono Y., Matsumoto H., Hayashi T., Ogawa T., Imai K., Kuzuhara T., Nishizono A., Fujiki H. TNF-alpha-inducing protein, a carcinogenic factor secreted from H. pylori, enters gastric cancer cells. Int. J. Cancer. (2008) 123:117-22.
Kuzuhara T, Suganuma M, Fujiki H. Green tea catechin as a chemical chaperone in cancer prevention. Cancer Lett. (2008) 261(1):12-20.
Kuzuhara T., Suganuma M., Oka K., Fujiki H. DNA-binding activity of TNF-alpha inducing protein from Helicobacter pylori. Biochem Biophys Res Commun. (2007) 362:805-10.
Kuzuhara T., Tanabe A., Sei Y., Yamaguchi K., Suganuma M., Fujiki H. Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins. Mol Carcinog. (2007) 46:640-645.
Kuzuhara T, Suganuma M, Kurusu M, Fujiki H. Helicobacter pylori-secreting protein Tipalpha is a potent inducer of chemokine gene expressions in stomach cancer cells. J. Cancer. Res. Clin. Oncol. (2007) 133:287-296.
Kuzuhara T., Sei Y., Yamaguchi K., Suganuma M., Fujiki H. DNA and RNA as new binding targets of green tea catechins. J. Biol. Chem. (2006) 281:17446-56.
Suganuma M, Kuzuhara T, Yamaguchi K, Fujiki H. Carcinogenic role of tumor necrosis factor-alpha inducing protein of Helicobacter pylori in human stomach. J. Biochem. Mol. Biol. (2006) 39:1-8.
Kuzuhara T., Suganuma M., Tsuge H., Fujiki H. Presence of a motif conserved between Helicobacter pylori TNF-alpha inducing protein (Tipalpha) and penicillin-binding proteins. Biol. Pharm. Bull. (2005) 28:2133-7.