Miniaturized System for Live-Cell Label-free Imaging Based on Fourier Ptychographic Microscopy

Kyungwon Lee, Kyung Chul Lee, Jaewoo Jung and Seung Ah Lee

We built a miniaturized system based on Fourier Ptychographic Microscopy (FPM) technique for live-cell imaging in an incubator. This system is made in a compact size by using a simple configuration and an objective lens with a low NA value. Label-free image acquisition is possible through multiple images obtained from a light source irradiating the sample from various angles and Quantitative Phase Imaging (QPI) through an iterative phase retrieval algorithm. By combining these features, we have effectively acquired images of HeLa cells in the incubator. We were able to acquire images of a large space-bandwidth product (SBP) with a FoV of 12mm2 and an axial resolution of 630nm. 

Long-term time-lapse images of HeLa cells were acquired in real time using our setup. This method can be effectively used in fields such as drug screening or diagnosing a patient's disease.

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