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Biological Question
Biological Question
What are the changes in gene expression that exist between leaves and shoot apical meristems in Heinz 1706 Tomato?
Experimental Approach
Experimental Approach
Before the session began, samples of S. lycopersicum shoot apical meristem and leaf tissue were taken. Each sample was sequenced by Illumina paired-end with a coverage of 50X. The sequencing data was analyzed using comparative plant transcriptomic. The reads were aligned to Heinz 1706 v5.0 Tomato transcriptome. The following image, produced using BioRender, displays the experimental process:
GALAXY platform was used to perform differential gene expression analysis
ACKNOWLEDGEMENTS
ACKNOWLEDGEMENTS
BITCPT Instructors
Course designer and Instructor: Dr. Carly Sjogren, B.A. Biology, Ph.D. Genetics, Genomics & Bioinformatics
Assistant Instructor: Dr. Emily Delorean, B.S. Crop Science, M.S. Plant Pathology, Ph.D. Genetics
Graduate Teaching Assistant: Sahana Prakash, B.S. Biological Sciences, in progress M.S. Physiology
Team Genes members
Marissa Silvestri
Matt Farrel
Sai Karthik
NC State HPC & bioinformatic resources
REFERENCES
REFERENCES
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