Plant hormonal signalling group
Charles University (Prague, Czech Republic) | Faculty of Science | Department of Experimental Plant Biology | Plant Cell Biology and Biotechnology
News & Media
26. 6. 2023 Anna Kampová presented her poster on anther dehiscence regulation at Mendel Early Career Symposium in Vienna.
20. 2. 2023 Auxins and Cytokinins in Plant Development Symposium will be held in Prague in June 25-29, 2023, more info
19. 1. 2023 Ayoub Stelate successfully defended his dissertation thesis titled Cellular determinants of the distribution of PIN auxin transporters in the plasma membrane.
12. 9. 2022 Štěpánka Kebrlová successfully defended her diploma thesis titled The role of actin cytoskeleton in the targeting of auxin carriers to the plasma membrane.
28. 9. 2021 Ayoub Stelate published his resulsts of super-resolution microscopy on auxin carriers in Biomolecules
Older news...
14. 9. 2021 Katarina Kurtović presented her poster on phytohormonal study in Chara at MAdLand Annual Meeting 2021 in Schwarzwald [DE]
7. 9. 2021 Katarina Kurtović talked about phytohormonal study in Chara and Anna Kampová won 3rd price with poster on the anther opening at 16th Student Days of Plant Biology CS 2021
28. 6. 2021 Anna Kampová presented her poster on anther dehiscence at Plant Biology Europe 2021
10. 9. 2020 Anna Kampová defended her diploma thesis about The role of atmospheric precipitation in anther dehiscence
13. 2. 2020 Stanislav Vosolsobě described the plant origin in the paper When a plant became a plant for the Živa journal [in Czech]
16. 12. 2019 Stanislav Vosolsobě wrote about our publication PIN-driven auxin transport emerged early in streptophyte evolution for the magazine Přírodovědci.cz [in Czech]
25. 8. 2019 Anna Kampová and Ayoub Stelate presented their results at the conference Plant Biology CS in České Budějovice (CZ)
Our mission
We are fascinated by the world of plant hormonal regulation, especially by the auxin flow, which regulates the plant development during its entire journey from plant tips to roots.
Our team is interconnected with the Laboratory of hormonal regulations in plants, Czech Academy of Science in Prague.
We are using a broad spectrum of molecular biology approaches (protein localisation by fluorescent tags, CRISPR/Cas9 mutagenesis, NGS transcriptomics), classical laser confocal and light-sheet microskopy, super-resolution techniques and bioinformatics (big data analysis by cluster computing)
We investigate the sub-cellular localization of auxin transporters and the role of the cytoskeleton and Arp2/3 complex in the auxin transporters positioning.
We deal with the reconstruction of auxin signalling evolution by EVO-DEVO study of the closest relatives of algae to land plants (mainly stoneworts).
We study complex regulatory mechanisms involved in the control of anther dehiscence with respect to the influence of weather effect on the whole process.
Open positions
We are open to any international co-operation concerning to our topics.
If you are interested in PhD or Postdoc position in our team, please contact us! You can join one of our topic:
Localisation of auxin transporters, their interaction with cytoskeleton
Evolutionary history of auxin signalling and transport, the role of auxin (and other phytohormones) in algae
Anthers opening regulation and EVO-DEVO study of pistils and sporangia of cryptogamous plants
What you can learn from us?
Plant cultivation in vitro ⚫ Gene cloning and tagged protein preparation ⚫ Plant transgenesis ⚫ Laser scanning microscopy ⚫ Scanning electron microscopy ⚫ Proteomic analysis ⚫ Metabolic profiling of plant growth regulators ⚫ Image analysis ⚫ Sequence analysis ⚫ Phylogenetic analysis ⚫ Data analysis in R environment ⚫ Grid computing ⚫ BASH scripting
Our models
Arabidopsis thaliana ⚫ Nicotiana tabacum BY-2 suspension cells ⚫ Arabidopsis arenosa and lyrata ⚫ Chara braunii and other algae
People
RNDr. Jan Petrášek, Ph.D.
Leader Since his graduation of Charles University under supervision by Eva Zažímalová and Zdeněk Opatrný with thesis Hormonal regulation of plant cell division and elongation (2007) Jan's works is devoted to study of regulation and localisation of auxin transporters using advanced microscopical approaches. Afterwards, his interest has spread to auxin signalling evolution and comparative study of plant and algae.
Lectures Practical basics of scientific work (MB130P16), Plant growth a development (practical courses, only in Czech language) (MB130C78), Plant Physisology (MB130P16E)
Publications Google Scholar
Contact petrasek@ueb.cas.cz
Consulting hours on Tuesday and Friday after previous agreement
Mgr. Stanislav Vosolsobě, Ph.D.
Postdoc Graduated on Charles University under supervision by Kateřina Schwarzerová and Jan Petrášek with thesis Evolutionary study of membrane proteins (2019) and deals with evolutionary-developmental biology, especially the study of the evolution of auxin signalling by bioinformatic approaches.
Lectures Stories from plant signalling evolution (MB130P89) only in Czech language
Publications Google Scholar
Contact vosolsob@natur.cuni.cz
Consulting hours after previous agreement
Mgr. Pavel Jelínek
PhD student
Topic
Supervisor Jan Petrášek
Katarina Kurtović, M.Sc.
PhD student Completed her Master's programme with the thesis ''Regeneration of Istrian ecotypes of garlic through somatic embryogenesis'' at Faculty of Agriculture, University of Zagreb, Croatia.
Topic The role of auxin in Chara braunii
Supervisor Stanislav Vosolsobě / Jan Petrášek
Mgr. Anna Kampová
PhD student completed her Master's study at CharlesUniversity, Faculty of Science with the thesis on the role of atmospheric precipitation in anther dehiscence
Topic Mechanism of anther dehiscence regulation
Supervisor Stanislav Vosolsobě
Contact anna.kampova@natur.cuni.cz
Twitter https://twitter.com/AKampova
Alumni
Mgr. Štěpánka Kebrlová
Defended 2022
Topic The role of actin cytoskeleton in the targeting of auxin carriers to the plasma membrane
Supervisor Jan Petrášek
Bc. Ema Albrechtová
Defended 2022
After defense of her bachelor thesis, she continued in master's study at Leyden University
Topic Thermoreception in plants
Supervisor Stanislav Vosolsobě
Ayoub Stelate, Ph.D.
Defended 2023
PhD student in STARS programme. Completed his Master of Science at Faculty of Science University Mohammed V in Rabat, Morocco. His master’s degree final project ''the phylogenetic relationship between Parmotrema crinitum and Parmotrema perlatum'' was done in collaboration with University Complutense in Madrid, Spain.
Publications ResearchGate
Topic Cellular determinants of auxin carrier dynamics
Supervisor Jan Petrášek
Methods
ImageJ macro for surface and interior extraction from confocal images
Method for automated kymograph analysis
Publications
A Stelate, E Tihlaříková, K Schwarzerová, V Něděla, J Petrášek (2021): Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules 11(10), 1407
S Vosolsobě, R Skokan, J Petrášek (2020): The evolutionary origins of auxin transport: what we know and what we need to know. Journal of Experimental Botany 71 (11), 3287–3295
J García-González, Š Kebrlová, M Semerák, J Lacek, I Kotannal Baby, J Petrášek, K Schwarzerová (2020): Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis. Frontiers in Plant Science 11, 486
R Skokan, E Medvecká, T Viaene, S Vosolsobě, M Zwiewka, K Müller, … J Petrášek, J Friml (2019): PIN-driven auxin transport emerged early in streptophyte evolution. Nature plants 5 (11), 1114-1119
K Müller, P Hošek, M Laňková, S Vosolsobě, K Malínská, M Čarná, … J Petrášek (2019): Transcription of specific auxin efflux and influx carriers drives auxin homeostasis in tobacco cells. The Plant Journal 100 (3), 627-640
T Nishiyama, H Sakayama, J De Vries, H Buschmann, D Saint-Marcoux, … S Vosolsobě, … J Petrášek, … S Rensing (2018): The Chara genome: secondary complexity and implications for plant terrestrialization. Cell 174 (2), 448-464. e24
S Vosolsobě, K Schwarzerová, J Petrášek (2018): Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins. JoVE (Journal of Visualized Experiments), e57837
S Vosolsobě, J Petrášek, K Schwarzerová (2017): Evolutionary plasticity of plasma membrane interaction in DREPP family proteins. Biochimica et Biophysica Acta (BBA)-Biomembranes 1859 (5), 686-697
Contact
Jan Petrášek petrasek@ueb.cas.cz
Stanislav Vosolsobě vosolsob@natur.cuni.cz
Phone
+420 22 195 1695
Mailing address
Department of Plant experimental biology
Charles University, Faculty of Science
Viničná 5
128 44 Prague 2
Czech Republic
Place
We are sitting in the Laboratory No. 002
(Viničná 5 building, ground floor, left of the main door)