DNA Replication & Polymerase Chain Reaction (PCR)

Learning Guideline:

1. Semiconservative DNA replication model

2. Meselson–Stahl experiment

3. DNA replication: 5’ à 3’ direction, DNA polymerase III

4. Primase & Primer

5. Leading strand & Lagging strand (Okazaki fragments)

6. DNA polymerase I & DNA ligase

7. Helicase, Topoisomerase, Single-strand binding protein

8. 補充 The end-replication problem: telomere & telomerase

9. PCR? – Amplify a specific region of DNA fragment

10. Heats? (Thermal cycle of 3 levels of temperatures)

11. DNA polymerase & Primers (forward primer and reverse primer)

12. 補充 How many PCR products (DNA fragments) can be amplified thru 35 cycles? 2n?

13. 補充 Why is Mullis considered as one of the most insane Nobel Prize Winners?

14. Site-directed mutagenesis? Primers? DpnI? Transformation?