Contents
Note: Updates made for 2025-26 are highlighted in yellow.
Students must be in grades 5-8 (or equivalent for homeschooled students). While a student in grade 5 can place in their category, only students in grades 6-8 can be considered for Thermo Fisher Scientific Junior Innovators Challenge, the national competition for middle school science fairs. The Coding with Commitment, Lemelson Young Inventor, and DoD STEM Leadership awards are only available to students in grades 6-8.
Students must live in Maine or attend a school in Maine. Written exception can be given to students from a state or region that does not have a JIC-Affiliated fair. Students participating in MMSSEF may not participate in any other JIC-affiliated fair. Violating this rule will result in disqualification.
Individual and team projects are eligible. Teams can have a maximum of three students for the project to qualify. Contact the MMSSEF Team if you would like to discuss an exception to this rule. See Section IV: Team Projects for more information.
Research conducted and presented at the Fair must have been properly supervised and approved according to the guidelines set forth by MMSSEF and must be in compliance with all local, state, and national laws and regulations. See Section V: Science Fair Registration for more information.
Each student may submit only one project.
Each student/team is required to complete all sections of the online application, which includes:
Permission Form (REQUIRED – Due March 1)
Research Plan (REQUIRED – Due March 1 for certain projects and April 18 for all others - see below)
250-word Abstract (REQUIRED – Due April 18)
Projects that involve certain activities may need to complete additional forms and submit them by March 1 for pre-approval. See Section V: Science Fair Registration for more information.
Each project should only include up to one year’s work and cannot have been previously entered in MMSSEF without meeting the continuation criteria. Projects that build on prior research must document that the additional research is a substantive expansion from prior work (e.g. testing a new variable or new line of investigation). Repetition of previous experimentation with the same methodology and research question, even with an increased sample size, is an example of an unacceptable continuation.
Each student/team must submit their entry by the final submission deadline of April 18.
Student researchers, as well as adults who have a role in their projects, are expected to maintain the highest ethical standards. These include, but are not limited to:
Integrity. Honesty, objectivity, and avoidance of conflicts of interest are expected during every phase of the research. The project should reflect independent research done by the student(s) and represent only one year’s work. If you use Artificial Intelligence, you must use it responsibly and not present work done by AI as your own.
Legality. Compliance with all federal, country, state and local laws is essential. All projects must be approved by a Scientific Review Committee (SRC), and when necessary must also be approved by an Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC), and/or Institutional Biosafety Committee (IBC).
Respect for Confidentiality and Intellectual Property. Confidential communications, as well as patents, copyrights, and other forms of intellectual property must be honored. Unpublished data, methods, or results may not be used without permission, and credit must be given to all contributions to research.
Stewardship of the Environment. It is the responsibility of the researcher(s) and the adults involved to protect the environment and its organisms from harm. All projects involve some amount of risk. Everyone is expected to recognize the hazards, assess the risks, minimize them, and prepare for emergencies.
Animal Care. Proper care and respect must be given to vertebrate animals. The guiding principles for the use of animals in research includes the following “Four R’s”: Replace, Reduce, Refine, Respect.
Human Participant Protection. The highest priority is the health and well-being of the student researcher(s) and human participants.
Potentially Hazardous Biological Agents (PHBAs). It is the responsibility of the student and adults involved in the project to conduct and document a risk assessment, and to safely handle and dispose of organisms and materials.
Scientific fraud and misconduct are not condoned at any level of research or competition. This includes plagiarism, forgery, use or presentation of other researcher’s work as one’s own and fabrication of data. Fraudulent projects will fail to qualify for competition in MMSSEF. MMSSEF reserves the right to revoke recognition of a project subsequently found to have been fraudulent.
The student and parent or guardian agree to this ethics statement by signing the Permission form found in the application.
Students can work individually or in teams with two or three members. Contact the MMSSEF Team to discuss an exception to this rule.
Each member of a team must register with parental permission.
Project materials (research plan, abstract, etc.) only need to be submitted once for each project.
Each team member should plan to participate in interviews with judges.
Each project needs a Visual Aid. The Visual Aid is for students to show any photos, charts, and tables related to their materials/methods, research data, and/or analysis used to support the findings of the project. Visual Aids must follow the guidelines below:
The recommended format is the Quad Chart. A Quad Chart template is available here. This can be used to print on a large-format printer or as a tool to plan the layout for a handmade visual aid. To create your own copy, go to File>Make a Copy. You can also download the template as a PowerPoint file by going to File>Download.
A large visual aid is recommended. Two common sizes are 24” tall × 36” wide and 36” tall × 48” wide. Guidelines:
The visual aid must stand on its own on a table. Students are advised to bring their own tabletop easels or use freestanding posters such as trifold boards.
The visual aid must be no larger than 48” wide by 48” tall.
Handmade posters are allowed and encouraged. Students can glue printed text and graphics onto sections of a poster board.
The following are not allowed within your visual aid:
Active hyperlinks to websites with additional information about your project.
Any videos or audio embedded within the Visual Aid.
Any identifiable photographs of yourself and/or any human subjects. Photographs, where faces are digitally blurred or covered, are acceptable.
Students may provide additional items with their display, such as a lab notebook or props. Please view the list of banned and restricted items below. When in doubt, contact the MMSSEF team to see if an item is allowed on display.
NOT ALLOWED at display:
Living organisms
Human or animal waste
Taxidermy specimens or parts
Preserved vertebrate or invertebrate animals
Human/animal parts or body fluids (for example, blood, urine)
All hazardous substances or devices (such as caustic chemicals, poisons, drugs, firearms, weapons, ammunition, reloading devices, grease/oil, sublimating solids)
Items that may have been in contact with hazardous chemicals
Sharp items (for example, syringes, needles, pipettes, knives)
Glass
Flames and highly flammable materials
Batteries with open-top cells or wet cells
Drones or any flight-capable apparatus unless the power source removed
3D Printers unless the power source is removed
Inadequately insulated apparatus capable of producing dangerous temperatures are not permitted
Any apparatus with belts, pulleys, chains, or moving parts with tension or pinch points that are not appropriately shielded
Any display items that are deemed distracting to other participants (i.e. sounds, lights, odors)
Any apparatus or project material deemed unsafe by MMSSEF staff
Allowed at the discretion of MMSSEF staff:
Certain living, dead, or preserved plant materials
Certain soil, sand, rock, and/or cement
Certain human or animal food
Registration for MMSSEF has multiple steps.
Teacher registers in LaunchPad and provides their signature.
Each student registers in LaunchPad.
Student registration triggers an email to the parent/guardian who gives permission.
Each student/team creates their project in LaunchPad. This includes providing their Research Plan. Students can create a copy of this document to build their research plan.
For projects requiring Advance Review, students submit necessary forms. For team projects, only one set of forms is needed per project.
MMSSEF will inform teachers if a project is approved or if it needs modifications before the students can begin experimentation.
Final Submission includes the Title, Category, Research Plan, and Abstract. For team projects, only one submission is needed per project.
Abstract: A narrative no longer than 250 words that describes the following aspects of the project:
Purpose - background, namely the reason, for investigating the project topic and a statement of the problem the research is looking to solve or the questions being tested.
Procedure - how the investigation was conducted, highlighting key points, and including methods and resources used.
Observations/Data/Results - key results that lead directly to the conclusions drawn.
Conclusions - a short summary in 1-2 sentences reflecting on the research process and results, which may include conclusive ideas, important applications, and implications of the research.
Do not include tables, images, graphs, or a bibliography.
Some projects, including those involving humans, vertebrate animals, or potentially hazardous biological agents, require pre-approval by the Scientific Review Committee (SRC) and/or Institutional Review Board (IRB). The remainder of this document outlines this Advance Review process.
The following information will help teachers and students determine whether projects need additional forms for Advance Review or if they can take the Fast Track to project submission.
If you answered Yes, review the Fast Track Quick Reference on the following page to determine if the activities are exempt.
*Safety Assessment must be conducted and included in procedures
Middle school teachers and students can use the Fast Track rules of the MMSSEF for basic investigations. These rules and their paperwork are designed to cover the most ethically clear and least hazardous situations. Some students will want to do more complex investigations and they will be covered by the more detailed Advance Review rules and forms put forth by the International Science & Engineering Fair (ISEF). The following is intended to outline which set of rules applies to which situations. Please contact the SRC with your questions at sciencefair@mmsa.org.
While some projects are not allowed under Fast Track Rules, middle school students still may do research involving some of these situations if they receive pre-approval from MMSSEF, follow ISEF rules, and submit all the required ISEF forms. Please read through the ISEF rules before experimentation begins. Most ISEF forms need to be filled out and signed before experimentation begins.
Fast Track projects can include observational studies of legal public behavior of children and/or adults where there is NO interaction between the researcher (or someone acting on behalf of the researcher) and their subjects. For example, it is acceptable for a student to observe how many children play on the monkey bars vs. the slide at the park but it is not allowed if a student observes how many children play on the monkey bars vs. the slide at the park and then asks the children why they prefer one over the other. A researcher may not manipulate an environment to observe how people respond to the manipulation. It is also unacceptable for a teacher to administer a survey or a test to their class on behalf of the researcher. It is acceptable to use data from the internet that is publicly available for analysis.
Human Subjects projects not allowed under Fast Track Rules
Eating, drinking, or tasting anything, including food, candy or water
Exercise studies
Surveys or tests
Consumer products testing involving human subjects
Taking fingerprints
Measuring heart rates
Projects involving the use of hazardous chemicals and devices and involvement in hazardous activities require direct supervision by a parent or teacher. Hazardous chemicals and compounds include acids, bases, and alcohol. This includes household items like bleach, over-the-counter medicines, fertilizers and manure. Hazardous activities are those that involve a level of risk above and beyond that encountered in the student’s everyday life. When in doubt, ask MMSSEF staff. Hazardous devices include laboratory equipment and power tools that require a moderate to high level of expertise to ensure safe usage. Solid rocket engines when unaltered and used according to manufacturer’s directions are allowed as long as safety assessment includes adult supervision.
Not allowed under Fast Track Rules
Firearms, explosives, fireworks, fire and fire extinguishers
Class III and IV lasers
DEA controlled substances, Prescription drugs and Tobacco
Radiation
Chemicals with a pH of 1 or 14 (very strong acid or base)
Liquid nitrogen
Pressurized gases
Two types of Vertebrate animal projects are allowed using the MS Fast Track form.
Observational studies of behavior of animals in their habitat, including the home for pets and the zoo and nature for wild animals, where there is NO intervention or treatment. OK: a student observes goldfish behavior during feeding time vs. non-feeding times on a normal feeding schedule. Not allowed: a student observes how the goldfish react to living in a dark closet.
Behavioral projects for pets involving doing things that pets experience in everyday life such as a new food dish, supplemental treats (following label recommendations), a new toy. OK: a student observes which colored dish a dog prefers to drink from. Not allowed: adding food coloring to water to see which color the dog prefers.
Pets are defined as animals not acquired specifically for a research project. Using pets owned by other people is allowed, as long as the owner of the animal is present.
Advance Review Checklist for Vertebrate Animals
If any of the following statements are true, Advance Review forms and rules must be used. Any projects that end in death will not be allowed at the fair.
I will buy an animal to experiment on.
I will feed the animal food, vitamins or supplements not labeled for it.
The animal died during this project.
The animal got sick during this project.
This activity is not normally performed by this type of animal. (for example: fish swim, cats don’t)
This activity will cause the animal stress or fear.
Safety Assessment for Vertebrate Animals
The following safety assessment questions need to be included in the project procedures.
What type and how many animals will be used?
Who will take care of the animals?
What will happen to the animals after the experiment?
The following human and animal tissues are allowed using the Fast Track form.
Hair, hooves, nails and feathers
Sterilized teeth
Meat, meat by-products, pasteurized milk, or eggs obtained from a food store and not consumed
Commercially prepared fixed tissue slides
The following microbes are approved without special precautions, but tasting the product as part of the experiment is not allowed:
Baker’s or Brewer’s yeast purchased from a store
Studies involving Lactobacillus, nitrogen-fixing, oil-eating bacteria and algae-eating bacteria introduced into their natural environment. These are not exempt if cultured in a petri dish environment, ISEF rules must be used.
Studies of mold growth on food items if the experiment is stopped at the first sign of mold.
Studies of mushrooms and slime mold.
The following microbe projects can only be conducted at school or a research lab following Bio Safety Level 1 protocols as stated for unknown specimens:
Decomposition or mold growth experiments either on nonfood items or those that continue beyond the first sign of mold on food
Unknown specimens obtained from the environment, not a living creature
Bio Safety Level 1 microbes specifically listed below
Regarding Unknown Specimens
Studies involving unknown microorganisms present a challenge because the presence, concentration and pathogenicity of possible agents are unknown. In science fair projects these studies typically involve the collection and culturing of microorganisms from the environment like soil, household surfaces, water, etc. Research with unknown microorganisms can be treated as a BSL-1 study under the following conditions:
The organism is cultured in a plastic Petri dish or other standard non-breakable container and sealed. Other acceptable containment includes petro film and doubled heavy-duty (2-ply) sealed bags.
The experiment involves only procedures in which the Petri dish remains sealed throughout the experiment, for example counting the presence of organisms or colonies.
The sealed Petri dish is disposed of in the appropriate manner by autoclaving or bleach solution by the teacher or Designated Supervisor.
All BSL-1 containment procedures are followed.
Regarding Bio Safety Level 1 Microbes
The only BSL-1 organisms approved for middle school use under the MSEZ rules are: Escherichia coli strain K12 and Pseudomonas fluorescens. All BSL-1 containment procedures must be followed. BSL-1 containment is normally found in water-testing laboratories, in high schools, and in colleges teaching introductory microbiology classes. Work is done on an open bench or in a fume hood. Standard microbiological practices are used when working in the laboratory. Decontamination can be achieved by treating with chemical disinfectants or by steam autoclaving. Lab coats are required and gloves recommended. The laboratory work is supervised by an individual with general training in microbiology or a related science.
Safety Assessment for Microbes
The following MSEZ safety assessment questions need to be included in the project procedures for every microbe experiment:
What types of microbes are involved?
What risks are involved?
What safety precautions will be used to reduce risk?
What disposal methods will be used?
Where will the research be conducted?
Not allowed under Fast Track Rules
Opening a culture for identification, sub-culturing or isolation
Swabbing in an area with a high likelihood of fecal contamination i.e. bathrooms and litter boxes
Swabbing a person