Imidazoquinoline-based TLR-7/8 agonist polymer conjugate modulates inflammatory immunogenicity in response to small changes in temperature on a scale associated with pyrexia (ΔT = 1–2 °C)
Hyperthermia (T ≥ 40 °C) has minimal effect on Raw-Blue cell death attributed to high dose IMQ
Future Research
Add a fluorescent monomer to the polymer-TLR-7/8 conjugate to monitor its localization and uptake within cells
Determine if the attenuation of the adjuvant’s potency at high temperatures is reversible upon return to physiological temperature
NACE Competencies
Teamwork and Communication: In our lab, we support one another. Whether its helping someone work-up or monitor a reaction, cleaning glassware, teaching new students, we rely on our colleagues to complete tasks in order to obtain the best results. The Mancini Lab is composed of both synthesis and biological laboratories. The compound I synthesize are passed to the BioSpace for cell testing. Before ordering supplies from the storeroom, I always check if anyone needs anything.
Equity and Inclusion: Our lab is composed of individuals from multiple countries and most of us have difference religious backgrounds. I prioritize respecting other people's beliefs.
Technology: The Mancini Group prioritizes utilizing efficient purification techniques, such as flash chromatography, and analyzing synthesized products using NMR, UV-Vis, and NMR.
References
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(8) Wilson, D. S.; Hirosue, S.; Raczy, M. M.; Bonilla-Ramirez, L.; Jeanbart, L.; Wang, R.; Kwissa, M.; Franetich, J. F.; Broggi, M. A. S.; Diaceri, G.; Quaglia-Thermes, X.; Mazier, D.; Swartz, M. A.; Hubbell, J. A. Antigens Reversibly Conjugated to a Polymeric Glyco-Adjuvant Induce Protective Humoral and Cellular Immunity. Nature Materials 2019, 18, 175–185.
(9) Perrier, S. 50th Anniversary Perspective: RAFT Polymerization–A User Guide. Macromolecules 2017, 50, 7433−7447.