Embedded Files
BL21 Lysate Fluorescence - LB Fluorescence
BL21 Lysate Fluorescence - LB Fluorescence
The plots show the difference in fluorescence between BL21 bacterial lysate and LB agar. Higher fluorescence in the BL21 samples indicates specific activation of DNAzymes by bacterial components, demonstrating sensor responsiveness.
Fluorescence Heatmap
Fluorescence Heatmap
The heatmap displays the initial fluorescence slopes for each DNAzyme-bacteria combination. Brighter colors represent faster reaction rates, helping visualize which DNAzymes respond most strongly to bacterial targets.
NACE Competencies
NACE Competencies
Critical Thinking & Problem Solving:
Through designing and analyzing a DNAzyme-based sensor array, I learned to interpret fluorescence data, troubleshoot inconsistencies, and identify meaningful patterns to assess bacterial detection accuracy. Developing the project on my own with my mentor, I continuously refined our experimental approach and methodology for optimal results.
Communication:
I translated complex data into clear visuals like kinetic plots and heatmaps, and practiced translating technical information into concise language for a scientific poster presentation. I also communicated regularly with my mentor and peers to develop the project and ensure its progress.
Equity & Inclusion:
My project focuses on developing low-cost, rapid diagnostics that could be used in under-resourced areas, demonstrating my commitment to making scientific tools more accessible and impactful for underserved communities.
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