The Aloe exudates were harvested by cutting the leaves of the plants and letting the yellow liquid drip out. We then used this exudate for further experiments as our treatment, along with the chemical components of aloin and aloenin. 

To test the microscopic morphological effects of the Aloe on the fungi, we made slide cultures. These consisted of a bottom coverslip, followed by a "core" with either just agar (control) or agar and our exudate/chemical (treatment), followed by a drop of our fungi, and lastly, the top coverslip. This "sandwich" of coverslips, core, and fungi was disassembled after the growing time and looked at under a microscope. 

To test how the Aloe affected the fungi's hyphal growth, we made colony diameter assays. These consisted of circles of water (control) or exudate (treatment) on an agar petri dish, with smaller circles of fungal inoculum on top. We then looked at these under a microscope to observe if the Aloe had any effect on the fungal growth diameter.