Example of Software Use with Existing Data

The Old

Here we have the Old LabVIEW software, with an immutable layout, every plot regardless of user need, clunky zoom and pan controls, no data labels, a frankly unappealing aesthetic, and a price tag. The new software developed here aims to address each of these issues while maintaining all of the functionality of this older software.

The New

Plotter Control Window

In this window, the user provides the software with the location of their data and selects basis spectra, plots to be made, the pixel range over which analysis is to be done, and the range to be considered background. The default values typically used for analysis of NADH fluorescence are shown. The most used plots are checked by default.

Basis Spectra

These basis spectra are used when calculating spectral decomposition. Here, the raw data are simply plotted so that the user can get a sense of the spectra as they are observed, before any processing might be done.

Intensity

Plotted here is the intensity of fluorescence over time. The two prominent spikes here are likely errors in data collection, but the overall shape gives insight. The increases in intensity around the eight and 30 minute marks are due to the build up of NADH resulting from the introduction of first a small, then large amount of cyanide. This cyanide makes the electron transport chain, first leaky, then disfunction, causing NADH resulting from the Krebs cycle to accumulate, and fluorescence to increase.

Centroid vs RMS

Plotted here is the centroid against the RMS of each spectrum. While not offering component information, the data here still offer further insight into the overall shape and distribution of each spectrum.

Spectral Phasor Analysis

The primary use of this software, the Spectral Phasor Analysis, is shown here. We can clearly see the non-linearity of the data, indicating that the metabolic pathways undertaken by mitochondria under the influence of small and large doses of cyanide differ.

Page updated

Report abuse