This experiment was repeated four times. After each trial, the results indicated areas of improvement, which the researchers took seriously by adjusting the procedure in order to produce better results with each trial. This involved identifying which specific steps of the procedure should be changed in order to produce better results, problem-solving how these steps could be changed, and performing multiple trials. The researchers had to accurately interpret the data and results after each trial and be realistic about the best way to modify the experiment.
The researchers collaborated for each experiment, which required constant communication as the procedure had specific time requirements. Teamwork was evident while the researchers were mounting the slides with the coverslips. Regardless of whether the slides were mounted under normal light, for the Giemsa stain, or with only red light, for the BODIPY stain, the researchers developed a system of mounting the slides that allowed each researcher to utilize their specific strengths. One researcher was in charge of micropipetting the mounting solution onto the slides while the other researcher removed the coverslips from the 24-well plates using tweezers and a bent needle, then placed the coverslips in the correct orientation onto the slides without breaking the coverslips in half. This required collaboration between the two researchers and consistent communication to ensure the proper procedure was maintained. Since both researchers had a similar goal of gaining research experience applicable to their future careers, collaboration was successful. Each researcher used their strengths during the research project, allowing one another to gain opportunities applicable to their future careers.
Chlamydia disproportionately affects young, low-income women (dos Santos et al., 2024). Plausibly, high incidence rates of chlamydia have been correlated with risky sexual behavior, limited healthcare access, and lower socioeconomic status. Additionally, chlamydia cases are often asymptomatic, at an average of 80%, making this disease prone to being transmitted unknowingly (dos Santos et al., 2024). Those with the disease lack symptoms and thus do not often seek a diagnosis or treatment. This research was dedicated to improving our understanding of the pathology of chlamydia, which is an important part of the research process towards developing more effective diagnostic tools, preventative care, and treatment methods against this sexually transmitted infection. Therefore, this research project acknowledged the demographics disproportionately impacted by this global disease. Through experimentation, pertinent information about chlamydial infections was analyzed, which helped address this health inequity.
For this research project, the use of a UV-light microscope was required to analyze the C5-BODIPY-ceramide-labeled HeLa cells in a dark room. Prior to this research project, the researchers had no experience using this specific microscope. In order to effectively analyze the results of their experiment, the researchers had to become fluent in using this technology. Not only did they learn how to visualize the cells using this specific microscope, but they also learned how to switch between different colored lights and how to take pictures of the results. Additionally, they learned how to increase the quality of the photos by adjusting the exposure or focus on the AmScope software.