Continue to produce mucus images to quantify thickness and goblet cell size
Optimization of FISH + lectin fluorescent staining procedure
Quantify and map the bacterial populations' spatial arrangements and its relation to mucus thickness
Produce triplicate results of each mouse
Optimization of Procedures:Â
We found that Methanol Carnoy's fixative prevents insulin antibody binding
UEA-1 agglutinin lectin staining is more effective than the MUC2 antibody for detecting the mucus layer