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All AP incubated at 70℃ denatured and had no activity by the 24 hrs mark
AP with pNIPAM seems to have the best activity, at all temperatures and almost all time points
Increases in enzymatic activity from pNIPAM are statistically significant at all time points for the room temp. and 37℃ incubations and at 0 and 24 hrs for the 55℃ incubation
The trehalose glycopolymer protects enzymatic activity at room temp. but appears to have no effect at 37℃ and 55℃
Protection of enzymatic activity from the trehalose glycopolymer is statistically significant at 0 and 72 hrs for the room temp. incubation
Copolymer #2 (randomized) has the second-best activity at room temp.
Increases in enzymatic activity from copolymer #2 are statistically significant at almost all time points for the room temp. incubation
There are statistically significant decreases in activity with copolymer #2 at 24 and 48 hrs for the 55℃ incubation
Copolymer #1 has the second-best activity at 55℃
There is a statistically significant increase in enzymatic activity from copolymer #1 at 0 hrs for the 55℃ incubation
Results at 37℃ are a bit mixed, but the activity of AP with the copolymers seems to be slightly higher than the activity of the two controls and AP with the trehalose glycopolymer
There are statistically significant decreases in activity with trehalose sugar, the trehalose glycopolymer, and AP by itself at 24, 48, and 72 hrs for the 55℃ incubation
Figure 4-A
Figure 4-A
Normalized average absorbances of AP using Quanti-Blue incubated at room temp. with various polymers and controls for 72 hours total. Error bars are standard deviations of triplicate absorbance measurements. Significant data is denoted by red asterisks, the reference is denoted by a red R.
Figure 4-B
Figure 4-B
Normalized average absorbances of AP using Quanti-Blue incubated at 37℃ with various polymers and controls for 72 hours total. Error bars are standard deviations of triplicate absorbance measurements. Significant data is denoted by red asterisks, the reference is denoted by a red R.
Figure 4-C
Figure 4-C
Normalized average absorbances of AP using Quanti-Blue incubated at 55℃ with various polymers and controls for 72 hours total. Error bars are standard deviations of triplicate absorbance measurements. Significant data is denoted by red asterisks, the reference is denoted by a red R.
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