- Fill out paper for field trip
- Notes/Pics/Discussion: prep for 0.8% agarose gels
- Begin making gels
- Plasmid DNA paper model activity (Day 1)
[OCT 30, 2019 - MINIMUM DAY]
No School - fires
No School - fires
- Day 2 - Recombinant Plasmid Paper Model
- More gels
- Discussion on gel electrophoresis
- Collect Directed Rdg
- More gels
- Final Day - Recombinant Plasmid Paper Model
- draw a gel on the back of your paper
- collect when finished
[NOV 6, 2019 - MINIMUM DAY]
- Final review, then Quiz: Paper Model
- Discussion on Ch. 4.3/4.4
- DNA Replication
- Notes/Discussion/Illustration
- Replication Group Assessments
- While you wait:
- 1 bullet/paragraph, Sect. 6.1 to 6.4
- Answer Rev ?'s: 6.1 to 6.4
- Click "Pregnancy Test Tutorial" in the links column; use this to help you answer ?'s on pg. 173 in text
**FOCUS ON THE FOLLOWING IN CHAPTER SIX:**
- know what an Assay is
- know what an ELISA is
- Direct ELISA
- Indirect ELISA
- know what a Western Blot is and how it works
- Finish Replication Group Quizzes & Ch. 6 Work
- Discussion
[NOV 13, 2019 - MINIMUM DAY]
- Ch. 6 Open Note Quiz [in partners]
- Collect Ch. 6 work
- Video: "Hilleman"
- 30 facts, 1 per every 2 minutes
- Guest Speaker: Jason Comeaux
- ABE Lab 2 - Restriction Digest (pgs. B-15 to B-21)
- Title
- Background Summary - pg. B-15 (3-4 sentences)
- Draw Bam HI + Hind III Restriction Sites (pg. B-8)
- Illustrate both the pKAN-R & pARA plasmids (pg. B-15)
- Answer "Stop & Think", pg. B-16
- Glue in Materials + Procedure (pgs. B-17 + B-18)
- Answer both "Stop & Think's", pg. B-18
- Answer Ch. 2 Questions, pg. B-19
- ABE Lab 3 - DNA Ligation (pg. B-25 to B-33)
- Title
- WDYAK #1-4, pg. B-25
- Using pg. B-26: How have plasmids & restriction enzymes helped to launch a new era in genetic engineering?
- Illustrate the products in the K+ and A+ tubes from Lab 2 (use pg. B-28 for help)
- draw each fragment
- include the genes that are found on each fragment & the size of each fragment
- Using pg. B-30:
- Illustrate the recombinant plasmid pARA-R
- List the functions of all the genes found on this plasmid
- glue Methods (pg. B-31, B-32) into your lab notebook
- Answer S + T, pg. B-32
- Answer Ch. 3 Questions, pg. B-33
[NOV 20, 2019 - MINIMUM DAY]
- Practice loading gels
- Make more gels
- Continue working on ABE Lab 2 and Lab 3 documentation
- Finish ABE Labs 2 and 3; collect Lab Notebooks
- Discussion on Products of Lab 2 and Products of Lab 3
- Make more gels
FIELD TRIP - MOORPARK COLLEGE & AMGEN!!
ABE LAB 4, DAY 1
- Title
- Purpose of Lab 4 (use the Intro on pg. B-37 to help you answer this)
- Answer "Consider" on pg. B-40
- Illustrate and Describe the 3 configurations of plasmids you might find (refer to pg. B-41)
- Glue Materials/Methods
- Answer "Stop & Think" pg. B-45
ABE LAB 4, Day 2
- Use your gel photo to make full analysis of your gel results; draw your results on the provided gel diagram
- Answer Ch. 4 ?'s, pg. B-48
[DEC 4, 2019 - MINIMUM DAY]
- Quiz: ABE Labs 2, 3, and 4
ABE Lab 5
- Title
- Summary of Intro paragraph, pg. B-53
- WDYAK, ?'s 1-4, pg. B-53
- pg. B-54
- Answer these questions: a) What is a vector? b) Why is a plasmid an ideal vector?
- Answer Consider question #1 & #2
- pg. B-55
- Explain:
- How are bacterial cells prepared to perform transformation?
- What is meant when we call cells "competent cells"?
- Summarize pgs. B-58 + B-59
- Answer BTL ?'s 1 & 2 on pg. B-60
- Paste in Methods
ABE LAB 5, DAY 2
- Write an analysis of all three plates.
- Answer the Ch. 5 Questions on pg. B-66
- ABE Lab 6, Day 1
- Preparation of Overnight Culture
- In Lab Notebooks, Complete ABE Lab 6 Directed Rdg.
- ABE Lab 6, Day 2
- Title
- Directed Rdg
- Complete "WDYAK", pg. E-3 [#1, #2 only]
- pg. E-5:
- Answer "Consider"
- Summarize each of the four phases in bacterial growth
- pg. E-6 - Answer "Consider"
- pg. E-7:
- Answer the following question: What are the properties that lead to protein folding?
- Answer "Consider"
- pg. E-8 - Answer "Consider"
- Glue Methods
- Answer "S & T" in Methods, pg. E-12
[DEC 11, 2019 - MINIMUM DAY]
- ABE Lab 6, Day 3
- Answer both "S & T" in Methods, pg. E-14
- Ch. 6 questions, pg. E-16
**LAB NOTEBOOK DUE TOMORROW!!**
- Finish ABE Lab 6; collect Lab Notebooks
- ABE Review Guide
- Collect Rev Guide
- ABE Exam Jeopardy
- ABE Exam
[DEC 16, 2019 - RALLY SCHEDULE]
- ABE 2.0 Lab #1 - Cheek Cell Extraction
- ABE 2.0 Lab #2 - Verification of DNA Samples
- ABE 2.0 Lab #3 - Hae Digestion of DNA Samples
- ABE 2.0 - discussion on gel results from both labs
- Continue working on bookwork
[DEC 20, 2019 - MINIMUM DAY]
- Bookwork due!
- Day 1: Moorpark College Review
- Cheese Lab - Day 1
- Negative Control + One Variable (Buttermilk, Chymosin, Rennet)
- Measure time to curd
[JAN 8, 2020 - MINIMUM DAY]
- Cheese Lab - Day 2
- Spin Samples to separate curds from whey
- Measure Volume of Whey
- Graph Data
- Complete "REE - PE - PA"
- Collect Cheese Lab
- CHEESE FEST!!
- Discussion on some Moorpark Exam material
- More Moorpark Review
- Last day of Moorpark Review