Overview
Overview
Chemical footprinting has been used for over five decades now as a method for obtaining structural information on biomolecules. The idea of using X-rays for footprinting, however, is relatively new. X-ray footprinting was first conceived and tried at the National Synchrotron Light Source at Brookhaven National Laboratory. Since then, the technique has grown in popularity and has been used to study structure and dynamics in a wide range of protein and nucleic acid systems, revealing information on ligand binding, protein-protein interactions, protein-nucleic acid interactions, and even on systems as complex as in vivo ribosome assembly. In the last decade, we have introduced this method at the Advanced Light Source synchrotron at Berkeley Lab, making it the second synchrotron X-ray footprinting facility in the United States.
Chemical footprinting has been used for over five decades now as a method for obtaining structural information on biomolecules. The idea of using X-rays for footprinting, however, is relatively new. X-ray footprinting was first conceived and tried at the National Synchrotron Light Source at Brookhaven National Laboratory. Since then, the technique has grown in popularity and has been used to study structure and dynamics in a wide range of protein and nucleic acid systems, revealing information on ligand binding, protein-protein interactions, protein-nucleic acid interactions, and even on systems as complex as in vivo ribosome assembly. In the last decade, we have introduced this method at the Advanced Light Source synchrotron at Berkeley Lab, making it the second synchrotron X-ray footprinting facility in the United States.
