Publications

Original Research 

1. Sumitomo A, Siriwach R, Higuchi M, Ngo AQ, Tanaka N, Prasongtanakij S, Thumkeo D, Narumiya S.

Phenylalanine amide deriviatives promote FLG expression via AHR activation in normal human epidermal keratinocytes.

Journal of Pharmacological Sciences, in press

2. Punyawattananukool S, Matsuura R, Wongchang T, Katsurada N, Tsuruyama T, Tajima M, Enomoto Y, Kitamura T, Kawashima M, Toi M, Yamanoi K, Hamanishi J, Hisamori S, Obama K, Charoensawan V, Thumkeo D, Narumiya S.

Prostaglandin E2-EP2/EP4 signaling induces immunosuppression in human cancer by impairing bioenergetics and ribosome biogenesis in immune cells.

Nature Communications 15: 9464 (2024).

3. Sigler AL, Thompson SB, Ellwood-Digel L, Kandasamy A, Michaels-Foster MJ, Thumkeo D, Narumiya S, Del Alamo JC, Jacobelli J.

FMNL1 and mDia1 promote efficient T Cell migration through complex environments via distinct 

mechanisms.

Frontiers in Immunology 15: 1467415 (2024).

4. Akamine T, Terabayashi, T, Sasaki T, Hayashi R, Abe I,  Hirayama F,  Nureki S, Ikawa M, Miyata H, Tokunaga A, Kobayashi T, Hanada K, Thumkeo D, Narumiya S, Ishizaki T. 

    Conditional deficiency of Rho-associated kinases disrupts endothelial cell junctions and impairs     

    respiratory function in adult mice.

    FEBS Open Bio 14:  906-921 (2024).

5. Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, Terai K. 

    Calcium transients trigger switch-like discharge of prostaglandin E2 (PGE2) in an ERK- dependent   

    manner.

    Elife 12: RP86727 (2024).

6. Wonchang T, Pluangnooch P, Hongeng S, Wongkajornsilp A, Thumkeo D, Soontrapa K. 

     Inhibition of DYRK1B suppresses inflammation in allergic contact dermatitis model and  

     Th1/Th17 immune response.

     Scientific Reports 13: 7058 (2023). 

7. Siriwach R, Ngo AQ, Narumiya S, Thumkeo D.

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA 

     sequencing analysis.

     STAR Protocols 3: 101906 (2022).

8. Thumkeo D, Punyawatthananukool S, Prasongtanakij S, Matsuura R, Arima K, Nie H, Yamamoto R, Aoyama N, Hamaguchi H, Sugahara S, Takeda S, Charoensawan V, Tanaka A, Sakaguchi S, Narumiya S.

    PGE2-EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in 

    inflammatory tumor microenvironment.

    Cell Reports 39: 110914 (2022).

9. Kremer KN, Buser A, Thumkeo D, Narumiya S, Jacobelli J, Palenda R, Torres RM. 

LPA suppresses T cell function by altering the cytoskeleton and disrupting immune synapse 

formation.

PNAS 119: e2118816119 (2022).

10. Siriwach R, Ngo AQ, Higuchi M, Arima K, Sakamoto S, Watanabe A, Narumiya S, Thumkeo D.

Single-Cell RNA sequencing identifies a migratory keratinocyte subpopulation expressing THBS1 

in epidermal wound healing.

iScience 25: 104130 (2022).

11. Kundishora AJ, Peters ST, Pinard A, Duran D, Panchagnula S, Barak T, Miyagishima DF, Dong W, Smith H, Ocken J, Dunbar A, Nelson-Williams C, Haider S, Walker RL, Li B, Zhao H, Thumkeo D, Marlier A, Duy PQ, Diab NS, Reeves BC, Robert SM, Sujijantarat N, Stratman AN, Chen YH, Zhao S, Roszko I, Lu Q, Zhang B, Mane S, Castaldi C, Ló pez-Girá ldez F, Knight JR, Bamshad MJ, Nickerson DA, Geschwind DH, Chen SL; Storm PB, Diluna ML, Matouk CC, Orbach DB, Alper SL, Smith ER, Lifton RP, Gunel M, Milewicz DM, Jin SC, Kahle KT.

DIAPH1 variants in non-east asian patients with sporadic Moyamoya disease. 

JAMA Neurology 78: 993〜1003 (2021).

12. Abe I, Terabayashi T, Hanada K, Kondo H, Teshima Y, Ishii Y, Miyoshi M, Kira S, Saito S, Tsuchimochi H, Shirai M, Yufu K, Arakane M, Daa T, Thumkeo D, Narumiya S, Takahashi N, Ishizaki T.

Disruption of actin dynamics regulated by Rho effector mDia1 attenuates pressure overload-

induced cardiac hypertrophic responses and exacerbates dysfunction. 

Cardiovascular Research 117: 1103〜1117 (2021).

13. Schneider R, Deutsch K, Hoeprich GJ, Marquez J, Hermle T, Braun DA, Seltzsam S, Kitzler TM, Mao Y, Buerger F, Majmundar AJ, Onuchic-Whitford AC, Kolvenbach CM, Schierbaum L, Schneider S, Halawi AA, Nakayama M, Mann N, Connaughton DM, Klä mbt V, Wagner M, Riedhammer KM, Renders L, Katsura Y, Thumkeo D, Soliman NA, Mane S, Lifton RP, Shril S, Khokha MK, Hoefele J, Goode BL, Hildebrandt F.

DAAM2 variants cause nephrotic syndrome via actin dysregulation. 

American Journal of Human Genetics 107: 1113〜1128 (2020).

14. Thumkeo D, Katsura Y, Nishimura Y, Kanchanawong P, Tohyama K, Ishizaki T, Kitajima S, Takahashi C, Hirata T, Watanabe N, Krummel MF, Narumiya S.

mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by 

Zap70 at the immune synapse.

Science Advances 6: eaay2432 (2020).

15. Sumitomo A, Siriwach R, Thumkeo D (co-first author), Ito K, Nakagawa R, Tanaka N, Tanabe K, Watanabe A, Kishibe M, Ishida-Yamamoto A, Honda T, Kabashima K, Aoki J, Narumiya S.

LPA induces keratinocyte differentiation and promotes skin barrier function through the 

LPAR1/LPAR5-RHO-ROCK-SRF axis.

Journal of Investigative Dermatology 139: 1010〜1022 (2019).

16.  Lee J, Aoki T, Thumkeo D, Siriwach R, Yao C, Narumiya S.

T cell-intrinsic prostaglandin E2-EP2/EP4 signaling is critical in pathogenic TH17 cell- driven 

inflammation.

Journal of Allergy and Clinical Immunology 143: 631〜643 (2019).

17. Sakamoto S, Thumkeo D (co-first author), Ohta H, Zhang Z, Huang S, Kanchanawong P, Fuu T, Watanabe S, Shimada K, Fujihara Y, Yoshida S, Ikawa M, Watanabe N, Saitou M, Narumiya S.

mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-

actin bundles and indispensable for spermatogenesis and male fertility. 

PLoS Biology 16: e2004874 (2018).

18. Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.

Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural 

hearing loss.

EMBO Molecular Medicine 8: 1310〜1324 (2016).

19. Nomachi A, Yoshinaga M, Liu J, Kanchanawong P, Tohyama K, Thumkeo D, Watanabe T, Narumiya S, Hirata T.

Moesin controls clathrin-mediated S1PR1 internalization in T cells.

PLoS One 8: e82590 (2013).

20. Toyoda Y, Shinohara R, Thumkeo D (co-first author), Kamijo H, Nishimaru H, Hioki H, Kaneko T, Ishizaki T, Furuyashiki T, Narumiya S.

EphA4-dependent axon retraction and midline localization of Ephrin-B3 are disrupted in the 

spinal cord of mice lacking mDia1 and mDia3 in combination.

Genes to Cells 18: 873〜885 (2013).

21. Shimizu Y, Kiumura F, Kaku S, Izuno M, Tomita K, Thumkeo D, Murakami T. 

Successful delivery following ICSI with macrocephalic sperm head syndrome: a case report. 

Reproductive BioMedicine Online 24: 603-605, 2012.

22. Shinohara R, Thumkeo D, Kamijo H, Kaneko N, Sawamoto K, Watanabe K, Takebayashi H, Kiyonari H, Ishizaki T, Furuyashiki T, Narumiya S.

A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron 

precursors.

Nature Neuroscience 15: 373〜380 (2012).

23. Thumkeo D, Shinohara R, Watanabe K, Takebayashi H, Toyoda Y, Tohyama K, Ishizaki T, Furuyashiki T, Narumiya S.

Deficiency of mDia, an actin nucleator, disrupts integrity of neuroepithelium and causes 

periventricular dysplasia.

PLoS One 6: e25465 (2011).

24. Kamijo H, Matsumura Y, Thumkeo D, Koike S, Masu M, Shimizu Y, Ishizaki T, Narumiya S. 

Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II. 

Genes to Cells 16: 1012〜1021 (2011).

25. Thumkeo D, Shimizu Y, Sakamoto S, Yamada S, Narumiya S.

ROCK-I and ROCK-II cooperatively regulate closure of eyelid and ventral body wall in mouse 

embryo.

Genes to Cells 10: 825〜834 (2005).

26. Shimizu Y, Thumkeo D (co-first author), Keel J, Ishizaki T, Oshima H, Oshima M, Noda Y, Matsumura F, Taketo MM, Narumiya S.

ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of 

actomyosin bundles.

Journal of Cell Biology 168: 941〜953 (2005).

27. Thumkeo D, Keel J, Ishizaki T, Hirose M, Nonomura K, Oshima H, Oshima M, Taketo MM,

Narumiya S.

Targeted disruption of the rho-associated kinase 2 gene results in intrauterine growth 

retardation and fetal death.

Molecular and Cellular Biology 23: 5043〜5055 (2003).

Reviews

1. Prasongtanakij S, Soontrapa K, Thumkeo D.

The role of prostanoids in regulatory T cells and their implications in inflammatory diseases and cancers.

European Journal of Cell Biology 104: 151482 (2025).

2. Thumkeo D, Narumiya S. 

Opening the door to better aspirin. 

Structure 29: 200〜202 (2021).

3. Narumiya S, Thumkeo D. 

Rho signaling research: history, current status and future directions. 

FEBS Letters 592: 1763〜1776 (2018).

4. Thumkeo D, Watanabe S, Narumiya S. 

Physiological roles of Rho and Rho effectors in mammals. 

European Journal of Cell Biology 92: 303〜315 (2013).