Publication
Preprint
Goto Y**, Aoki K**. (**Corresponding Authors)
Development of optogenetic tools to manipulate cell cycle checkpoints
bioRxiv, 2020. doi: https://doi.org/10.1101/2020.06.22.166264Ashida K, Aoki K, Ito S.
Experimental evaluation of thermodynamic cost and speed limit in living cells via information geometry.
bioRxiv, 2020. doi:10.1101/2020.11.29.403097Itoh T, Kondo Y, Aoki K, Saito N.
Emergence of Bow-tie Architecture in Evolving Feedforward Networks.
bioRxiv. 2023. p. 2023.03.28.534501. doi:10.1101/2023.03.28.534501Sakai K*, Kondo Y*, Goto Y**, Aoki K**. (*equal contribution, **Corresponding Author)
Cytoplasmic fluidization triggers breaking spore dormancy in fission yeast.
bioRxiv. 2023. p. 2023.09.27.559686. doi:10.1101/2023.09.27.559686Brumbaugh-Reed EH, Aoki K, Toettcher JE.
Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag.
bioRxiv. 2024. p. 2024.01.16.575860. doi:10.1101/2024.01.16.575860
2024
Sugiyama H*, Goto Y*, Kondo Y, Coudreuse D, Aoki K. (*equal contribution)
Live-cell1imaging defines a threshold in CDK activity at the G2/M transition.
Developmental Cell 2024. doi:10.1016/j.devcel.2023.12.014
プレスリリース(NIBB, ExCELLS)Sakai K, Aoki K**, Goto Y**. (**Corresponding Authors)
Live-cell fluorescence imaging and optogenetic control of PKA kinase activity in fission yeast Schizosaccharomyces pombe.
Yeast 2024. doi:10.1002/yea.3937
2023
Tanaka S, Aoki K, Arakawa K.
In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada.
Proc Natl Acad Sci U S A., 2023;120: e2216739120. doi:10.1073/pnas.2216739120
プレスリリース(ExCELLS, NIBB, 慶応義塾大学先端生命科学研究所), 【所内環境のみ接続可:2023.1.31 科学新聞】Kunida K, Takagi N, Aoki K, Ikeda K, Nakamura T, Sakumura Y.
Decoding cellular deformation from pseudo-simultaneously observed Rho GTPase activities.
Cell Reports, 2023; 112071. doi:10.1016/j.celrep.2023.112071
プレスリリース(ExCELLS, NIBB, NAIST, 藤田医科大学, 東京理科大学)Oda S*, Sato-Ebine E*, Nakamura A, Kimura KD, Aoki K. (*equal contribution)
Optogenetic control of cell signaling with red/far-red light-responsive optogenetic tools in Caenorhabditis elegans.
ACS Synthetic Biology, 2023.12; 700–708. doi:10.1021/acssynbio.2c00461
プレスリリース(ExCELLS, NIBB, 名古屋市立大学),【所内環境のみ接続可:2023.3.9 OPTRONICS ONLINE】Kamino K, Kadakia N, Avgidis F, Liu Z-X, Aoki K, Shimizu TS, Emonet T.
Optimal inference of molecular interaction dynamics in FRET microscopy.
Proc Natl Acad Sci U S A., 2023;120: e2211807120. doi:10.1073/pnas.2211807120青木一洋
総論 蛍光タンパク質センサーの現状と将来展望
月刊「細胞」2023年 4月臨時増刊号 蛍光タンパク質センサー後藤祐平、青木一洋
GPCRリガンドセンサーの開発
月刊「細胞」2023年 4月臨時増刊号 蛍光タンパク質センサーToyooka Y, Aoki K, Usami FM, Oka S, Kato A, Fujimori T.
Generation of pulsatile ERK activity in mouse embryonic stem cells is regulated by Raf activity.
Scientific Reports, 2023;13: 9465. doi:10.1038/s41598-023-36424-6Hirashima T, Hino N, Aoki K, Matsuda M.
Stretching the limits of extracellular signal-related kinase (ERK) signaling - Cell mechanosensing to ERK activation.
Current Opinion in Cell Biology, 2023;84: 102217. doi:10.1016/j.ceb.2023.102217Yagi-Utsumi M, Miura H, Ganser C, Watanabe H, Hiranyakorn M, Satoh T, Uchihashi T, Kato K, Okazaki K, Aoki K.
Molecular Design of FRET Probes Based on Domain Rearrangement of Protein Disulfide Isomerase for Monitoring Intracellular Redox Status.
International Journal of Molecular Science, 2023;24: 12865. doi:10.3390/ijms241612865Tomizawa Y, Minamino N, Shimokawa E, Kawamura S, Komatsu A, Hiwatashi T, Nishihama R, Ueda T, Kohchi T, Kondo Y.
Harnessing Deep Learning to Analyze Cryptic Morphological Variability of Marchantia polymorpha.
Plant Cell Physiology, 2023. doi:10.1093/pcp/pcad117Goh T, Song Y, Yonekura T, Obushi N, Den Z, Imizu K, Tomizawa Y, Kondo Y, Miyashima S, Iwamoto Y, Inami M, Chen Y-W, Nakajima K.
In-Depth Quantification of Cell Division and Elongation Dynamics at the Tip of Growing Arabidopsis Roots Using 4D Microscopy, AI-Assisted Image Processing and Data Sonification.
Plant Cell Physiology, 2023. doi:10.1093/pcp/pcad105Ikeda H, Uchikawa T, Kondo Y, Takahashi N, Shishikui T, Watahiki MK, Kubota A, Endo M.
Circadian Clock Controls Root Hair Elongation through Long-Distance Communication.
Plant Cell Physiology, 2023. doi:10.1093/pcp/pcad076Minamino N, Fujii H, Murata H, Hachinoda S, Kondo Y, Hotta K, Ueda T.
Analysis of plant-specific ANTH domain-containing protein in Marchantia polymorpha.
Plant Cell Physiology, 2023. doi:10.1093/pcp/pcad118Nakamura A, Goto Y**, Sugiyama H, Tsukiji S, Aoki K**. (**Corresponding Authors)
Chemogenetic Manipulation of Endogenous Proteins in Fission Yeast Using a Self-Localizing Ligand-Induced Protein Translocation System.
ACS Chemical Biology, 2023. doi:10.1021/acschembio.3c00478
プレスリリース(ExCELLS, NIBB)Otsubo Y, Yamashita A, Goto Y, Sakai K, Iida T, Yoshimura S, Johzuka K.
Cellular responses to compound stress induced by atmospheric-pressure plasma in fission yeast.
Journal of Cell Science, 2023. doi:10.1242/jcs.261292
プレスリリース(NIBB)Nakayama T, Tanikawa M, Okushi Y, Itoh T, Shimmura T, Maruyama M, Yamaguchi T, Matsumiya A, Shinomiya A, Guh Y-J, Chen J, Naruse K, Kudoh H, Kondo Y, Naoki H, Aoki K, Nagano A, Yoshimura T.
A transcriptional program underlying the circannual rhythms of gonadal development in medaka.
Proc Natl Acad Sci U S A., 2023;120: e2313514120. doi:10.1073/pnas.2313514120
プレスリリース(ExCELLS, NIBB)
2022
Aragaki H, Ogoh K, Kondo Y, Aoki K.
LIM Tracker: a software package for cell tracking and analysis with advanced interactivity.
Scientific Reports, 2022;12: 1–14. doi:10.1038/s41598-022-06269-6Tany R, Goto Y**, Kondo Y, Aoki K**. (**Corresponding Authors)
Quantitative live-cell imaging of GPCR downstream signaling dynamics.
Biochemical Journal, 2022;479: 883–900. doi:10.1042/BCJ20220021Suzuki S*, Nakamura A*, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S. (*equal contribution)
A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation.
Cell Chemical Biology, 2022. doi:10.1016/j.chembiol.2022.06.005Hu X, Roy SR, Jin C, Li G, Zhang Q, Asano N, Asahina S, Kajiwara T, Takahara A, Feng B, Aoki K, Xu C & Zhang Y.
Control cell migration by engineering integrin ligand assembly.
Nature Communications, 2022;13: 5002. doi:10.1038/s41467-022-32686-2Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M.
A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration.
Developmental Cell, 2022. doi:10.1016/j.devcel.2022.09.003山本 啓,近藤 洋平
ミオシンの光操作で明らかになった細胞質分裂における両極アクトミオシンの寄与
生物物理, 62(5),285-287(2022)DOI: 10.2142/biophys.62.285青木 一洋
思春期
生物物理, 62 (6) ,323 (2022)
2021
Goto Y, Kondo Y, Aoki K.
Visualization and Manipulation of Intracellular Signaling.
Adv Exp Med Biol., 2021;1293:225-234. doi: 10.1007/978-981-15-8763-4_13Yano T, Tsukita K, Kanoh H, Nakayama S, Kashihara H, Mizuno T, Tanaka H, Matsui T, Goto Y, Komatsubara A, Aoki K, Takahashi R, Tamura A, Tsukita S.
A microtubule‐LUZP1 association around tight junction promotes epithelial cell apical constriction.
The EMBO Journal, 2021;40. https://doi.org/10.15252/embj.2020104712Kajiwara K, Yamano S, Aoki K, Okuzaki D, Matsumoto K, Okada M.
CDCP1 promotes compensatory renal growth by integrating Src and Met signaling.
Life Sci Alliance, 2021;4. doi:10.26508/lsa.202000832Asakura Y, Kondo Y, Aoki K, Naoki H.
Hierarchical modeling of mechano-chemical dynamics of epithelial sheets across cells and tissue.
Scientific Reports, 2021;11: 4069. doi:10.1038/s41598-021-83396-6Farahani PE, Reed EH, Underhill EJ, Aoki K, Toettcher JE.
Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems.
Annu Rev Biomed Eng., 2021. doi:10.1146/annurev-bioeng-083120-111648Ito RE, Oneyama C, Aoki K.
Oncogenic mutation or overexpression of oncogenic KRAS or BRAF is not sufficient to confer oncogene addiction.
PLoS One, 2021 Apr 1;16(4):e0249388. doi: 10.1371/journal.pone.0249388. eCollection 2021.Wen C, Miura T, Voleti V, Yamaguchi K, Tsutsumi M, Yamamoto K, Otomo K, Fujie Y, Teramoto T, Ishihara T, Aoki K, Nemoto T, Hillman EM, Kimura KD.
3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images.
Elife, 2021;10: e59187. doi:10.7554/eLife.59187Nakamura A, Goto Y, Kondo Y, Aoki K.
Shedding light on developmental ERK signaling with genetically encoded biosensors.
Development, 2021;148. doi:10.1242/dev.199767Verma A, Jena SG, Isakov DR, Aoki K, Toettcher JE, Engelhardt BE.
A self-exciting point process to study multicellular spatial signaling patterns.
Proc Natl Acad Sci U S A., 2021;118. doi:10.1073/pnas.2026123118後藤 祐平, 青木 一洋
特集:ライブセルイメージング:細胞機能の可視化と操作
『ライブセルイメージングによる細胞内シグナル伝達の可視化と光操作』
顕微鏡. 2021 年 56 巻 2 号 p. 54-58. https://doi.org/10.11410/kenbikyo.56.2_54Matsui K, Okamoto K, Hasegawa T, Ohtsuka H, Shimasaki T, Ihara K, Goto Y, Aoki K, Aiba H.
Identification of ksg1 mutation showing long-lived phenotype in fission yeast.
Genes to Cells, 2021. doi:10.1111/gtc.12897Yagi-Utsumi M, Aoki K, Watanabe H, Song C, Nishimura S, Satoh T, Yanaka S, Ganser C, Tanaka S, Schnapka V, Goh EW, Furutani Y, Murata K, Uchihashi T, Arakawa K, Kato K.
Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade.
Scientific Reports, 2021;11: 1–9. doi:10.1038/s41598-021-00724-6
プレスリリース(ExCELLS, NIBB, IMS, NIPS, 名古屋大), EurekAlert!, 【所内環境のみ接続可:2021.11.8 NEWS SALT, TechCrunch】Nakamoto C*, Goto Y*, Tomizawa Y, Fukata Y, Fukata M, Harpsoe K, Gloriam D.E., Aoki K**, Takeuchi T**. (*equal contribution, **Corresponding authors)
A novel red fluorescence dopamine biosensor selectively detects dopamine in the presence of norepinephrine in vitro.
Molecular Brain, 2021;14: 1–14. doi:10.1186/s13041-021-00882-8
プレスリリース(ExCELLS, NIBB, NIPS),【所内環境のみ接続可:2021.12.20 OPTRONICS online】Hiiragi T, Gladfelter AS, Miguel-Aliaga I, Coller HA, Lau OS, McBride HM, Aoki K, Linkermann A, Santaguida S, Wasteneys G, Rivron NC, Shahbazi MN, Ebisuya M, Lancaster MA.
20 years of Developmental Cell: Looking forward.
Developmental Cell, 2021;56: 3185–3191. doi:10.1016/j.devcel.2021.11.017Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y**, Aoki K**. (**Corresponding Authors)
Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis.
Nature Communications, 2021;12: 1–13. doi:10.1038/s41467-021-27458-3
*Some plasmids used in this study are deposited in Addgene.
プレスリリース(ExCELLS, NIBB), EurekAlert!,【所内環境のみ接続可:2021.12.8 Phys.org その他8件, 2021.12.9 OPTRONICS online, 2021.12.10 日経バイオテク 】
総研大研究助成学生の研究紹介Sakai K, Kondo Y, Fujioka H, Kamiya M, Aoki K**, Goto Y**. (**Corresponding Authors)
Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system.
Journal of Cell Science, 2021;134. doi:10.1242/jcs.259315
第一著者の酒井くんが "First person" で紹介されました, Link
プレスリリース(ExCELLS, NIBB), 【所内環境のみ接続可:2021.12.21 OPTRONICS online】Nakayama H, Rowland SD, Cheng Z, Zumstein K, Kang J, Kondo Y, Sinha NR.
Leaf form diversification in an ornamental heirloom tomato results from alterations in two different HOMEOBOX genes.
Current Biology, 2021;31: 4788–4799.e5. doi:10.1016/j.cub.2021.08.023
2021年6月
青木が
生体の科学 Vol.72 No.3 2021年 06月号
「特集 生物物理学の進歩 —生命現象の定量的理解へ向けて—」を編集しました。
本書中には近藤と青木が担当した記事「情報理論による真核細胞の情報伝達機構の解析」も収録されています。
2020
青木一洋
フィトクロム B を用いた赤色光 / 遠赤色光による細胞内シグナル伝達系の光操作
光学, 49(1); 14-19, 2020.Nakamura A, Oki C, Kato K, Fujinuma S, Maryu G, Kuwata K, Yoshii T, Matsuda M, Aoki K, Tsukiji S
Engineering orthogonal, plasma membrane-specific SLIPT systems for multiplexed chemical control of signaling pathways in living single cells
ACS Chemical Biology, 2020, 15; 1004-1015. doi: 10.1021/acschembio.0c00024Yagi H, Yagi-Utsumi M, Honda R, Ohta Y, Saito T, Nishio M, Ninagawa S, Suzuki K, Anzai T, Kamiya Y, Aoki K, Nakanishi M, Satoh T, Kato K
Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor
Nature Communications, 2020, 11; 1368. doi: 10.1038/s41467-020-15192-1Hino N, Rossetti L, Marin-Llaurado A, Aoki K, Trepat X, Matsuda M, Hirashima T
ERK-mediated mechanochemical waves direct collective cell polarization
Developmental Cell, Volume 53, Issue 6, 22 June 2020, Pages 646-660.e8Akiyama S, Aoki K, Kubo Y
Biophysical research in Okazaki, Japan.
Biophysical Reviews, 15 Feb 2020, 12(2):237-243
DOI: 10.1007/s12551-020-00633-4 PMID: 32062838 PMCID: PMC7242583Uda Y*, Miura H*, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K. (*equal contribution)
Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics
ACS Chemical Biology 2020, 15, 11, 2896–2906, https://doi.org/10.1021/acschembio.0c00477
*Some plasmids used in this study are deposited in Addgene.
2019
Komatsubara A*, Goto Y*, Kondo Y, Matsuda M, Aoki K** (*equal contribution, **Corresponding Author)
Single-cell quantification of the concentrations and dissociation constants of endogeneous proteins
Journal of Biological Chemistry, 2019, 294; 6062-6072. doi: 10.1074/jbc.RA119.007685
*Some plasmids used in this study are deposited in Addgene.
基生研プレスリリース青木 一洋
ERK MAPキナーゼによる細胞増殖と細胞集団運動の制御
Journal of Japanese Biochemical Society 91(1); 73-80 (2019). doi: 10.14952/SEIKAGAKU.2019.910073近藤 洋平
動く組織の力学特性を測る
生体の科学 特集 メカノバイオロジー, 70(4); 284-289, 2019.後藤 祐平、青木 一洋
赤色光/遠赤色光による哺乳類培養細胞のシグナル伝達系の光操作
生物物理 59(6); 327-329, 2019. doi: 10.2142/biophys.59.327
2018
Oda S, Uda Y, Goto Y, Miura H, Aoki K (Corresponding Author)
Optogenetic Tools for Quantitative Biology: The Genetically Encoded PhyB–PIF Light-inducible Dimerization System and Its Application for Controlling Signal Transduction
Optogenetics, 2018; 137-148. doi: 10.1016/j.celrep.2018.08.020青木一洋
組織の秩序を保つ細胞の集団運動のしくみ
JT生命誌 季刊「生命誌」98号Miura H, Kondo Y, Matsuda M, Aoki K (Corresponding Author)
Cell-to-cell heterogeneity in p38-mediated cross-inhibition of JNK causes stochastic cell death
Cell Reports, 2018, 24; 2658-2668. doi: 10.1016/j.celrep.2018.08.020
*Some plasmids used in this study are deposited in Addgene.
基生研プレスリリース青木一洋、宇田燿一、小田茂和、後藤祐平
細胞外からの発色団添加を必要としない赤色光/近赤外光によるシグナル伝達系の光操作
実験医学「サイズ生物学」クローズアップ実験法, 36, 2018.Muta Y, Fujita Y, Sumiyama K, Sakurai A, Taketo MM, Chiba T, Seno H, Aoki K, Matsuda M, Imajo M
Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine
Nature Communications, 2018, 9; 2174. doi: 10.1038/s41467-018-04527-8Maryu G*, Miura H*, Uda Y, Komatsubara AT, Matsuda M, Aoki K (Corresponding Author)(* Equal contribution)
Live-cell imaging with genetically encoded protein kinase activity reporters
Cell Structure and Function, 2018, 43; 61-74. doi: 10.1247/csf.18003Kondo Y (Corresponding Author), Aoki K, Ishii S
Inverse tissue mechanics of cell monolayer expansion
PLoS Computational Biology, 2018, 14; e1006029. doi: 10.1371/journal.pcbi.1006029青木一洋
蛍光顕微鏡 ⅳ.FRET
実験医学増刊「生きてるものは全部観る! イメージングの選び方・使い方100+」第1章 光学顕微鏡, 36, 2018.
2017
Aoki K (Corresponding Author), Kondo Y, Naoki H, Hiratsuka T, Itoh RE, Matsuda M.
Propagating Wave of ERK Activation Orients Collective Cell Migration.
Developmental Cell, 2017, 43; 305–317. doi: 10.1016/j.devcel.2017.10.016
基生研プレスリリース、11月15日27面の日刊工業新聞、日経バイオテク青木一洋、宇田燿一、小田茂和
光誘導性二量体化による細胞内シグナル伝達経路の操作
生体の科学 増大特集細胞多様性解明に資する光技術─見て,動かす, 68(5); 492-493, 2017.Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K (Corresponding Author)
Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling
Proc. Natl. Acad. Sci. U.S.A., 2017, 114; 11962-11967. doi: 10.1073/pnas.1707190114
*Some plasmids used in this study are deposited in Addgene.
基生研プレスリリース、10月31日21面の日刊工業新聞、日経バイオテク
2016
Yamao M, Aoki K, Yukinawa N, Ishii S, Matsuda M, Naoki H
Two New FRET Imaging Measures: Linearly Proportional to and Highly Contrasting the Fraction of Active Molecules.
PLoS One. 2016, 11; e0164254. doi: 10.1371/journal.pone.0164254Kamezaki A, Sato F, Aoki K, Asakawa K, Kawakami K, Matsuzaki F, Sehara-Fujiwara A
Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo
Scientific Reports, 2016, 6; 28873. doi: 10.1038/srep28873Maryu G, Matsuda M, Aoki K (Corresponding Author)
Multiplexed fluorescence imaging of ERK and Akt activities and cell-cycle progression
Cell Structure and Function, 2016, 41; 81-92. doi: 10.1247/csf.16007. 2016年度日本細胞生物学会論文賞(CSF Award)受賞
*Some plasmids used in this study are deposited in Addgene.Inaba K, Oda K, Aoki K, Sone K, Ikeda Y, Miyasaka A, Kashiyama T, Fukuda T, Makii C, Arimoto T, Wada-Hiraike O, Kawana K, Yano T, Osuga Y, Fujii T.
Synergistic antitumor effects of combination of PI3K/mTOR and MEK inhibition (SAR245409 and pimasertib) in mucinous ovarian carcinoma cells by fluorescence resonance energy transfer imaging
Oncotarget, 2016, 7; 29577-29591. doi: 10.18632/oncotarget.8807青木一洋
細胞内情報伝達系の定量的なシミュレーション
生体の科学 増大特集 生命動態システム科学, 65 (5); 490-491, 2017.
2015
Nagata Y, Kontani K, Enami T, Kataoka K, Ishii R, Totoki Y, Kataoka TR, Hirata M, Aoki K, Nakano K, Kitanaka A, Sakata-Yanagimoto M, Egami S, Shiraishi Y, Chiba K, Tanaka H, Shiozawa Y, Yoshizato T, Suzuki H, Kon A, Yoshida K, Sato Y, Sato-Otsubo A, Sanada M, Munakata W, Nakamura H, Hama N, Miyano S, Nureki O, Shibat T, Haga H, Shimoda K, Katada T, Chiba S, Watanabe T, Ogawa S
Variegated RHOA mutations in adult T-cell leukemia/lymphoma
Blood, 2015, 127; 596-604. doi: 10.1182/blood-2015-06-644948Yamao M, Naoki H, Kunida K, Aoki K, Matsuda M, Ishii S
Distinct predictive performance of Rac1 and Cdc42 in cell migration
Scientific Reports, 2015, 5; 17527. doi:10.1038/srep17527Aoki K (Corresponding Author)
Visualization of Intracellular Signaling with Fluorescence Resonance Energy Transfer-Based Biosensors
Protein Modifications in Pathogenic Dysregulation of Signaling, 2015; 31-41. doi: 10.1007/978-4-431-55561-2Komatsubara AT, Matsuda M, Aoki K (Corresponding Author)
Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer
Scientific Reports, 2015, 5; 13283. doi:10.1038/srep13283Komatsu N, Fujita Y, Matsuda M, Aoki K (Corresponding Author)
mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogeneic KRas-mutant cancer cells
Oncogene, 2015, 34; 5607-5616. doi:10.1038/onc.2015.16Hiratsuka T, Fujita Y, Naoki H, Aoki K, Kamioka Y, Matsuda M
Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse epidermis
eLife, 2015, 4; e05178. doi: 10.7554/eLife.05178.001
2014
Sadaie W, Harada Y, Matsuda M, Aoki K (Corresponding Author)
Quantitative in vivo fluorescence cross-correlation analyses highlight the importance of competitive effect in the regulation of protein-protein interactions
Molecular and Cellular Biology, 2014, 34; 3272-3290. doi: 10.1128/MCB.00087-14Fujita Y, Komatsu N, Matsuda M, Aoki K (Corresponding Author)
FRET-based quantitative analysis of feedforward and feedback loops in EGFR signaling and the sensitivity to molecular targeting drugs
FEBS Journal, 2014, 281; 3177-3192. doi: 10.1111/febs.12852Miura H, Matsuda M, Aoki K (Corresponding Author)
Development of a FRET biosensor with high specificity for Akt
Cell Structure and Function, 2014, 39; 9-20. doi: http://doi.org/10.1247/csf.13018. 2014年度日本細胞生物学会論文賞(CSF Award)受賞
2014年11月26日
青木が小林徹也さん(東大金子生命動態)と一緒に編集した、
「バイオ画像手取り足取りガイド」が羊土社さんから出版されました。
画像処理の原理から、ImageJ, MetaMorph, Matlabによる画像解析の解析例付きです。
2013
Aoki K (Corresponding Author), Kumagai Y, Sakurai A, Komatsu N, Fujita Y, Shionyu C, Matsuda M
Stochastic ERK activation induced by noise and cell-cell propagation regulates cell density-dependent proliferation
Molecular Cell, 2013, 52; 529-40. doi: 10.1016/j.molcel.2013.09.015Aoki K (Corresponding Author), Takahashi K, Kaizu K, Matsuda M
A Quantitative Model of ERK MAP Kinase Phosphorylation in Crowded Media
Scientific Reports, 2013, 3; 1541. doi: 10.1038/srep01541Aoki K (Corresponding Author), Kamioka Y, Matsuda M
Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: Basis of biosensor construction, live imaging, and image processing
Development, Growth and Differentiation, 2013, 55; 515-522. doi: 10.1111/dgd.12039
2012
Kunida K, Matsuda M, Aoki K (Corresponding Author)
FRET imaging and statistical signalprocessing reveal positive and negative feedback loops regulating themorphology of randomly migrating HT-1080 cells
Journal of Cell Science, 2012, 125; 2381-2192. doi: 10.1242/jcs.096859Aoki K, Komatsu N, Hirata E, Kamioka Y, Matsuda M
Stable expression of FRET biosensors: A new light in cancer research
Cancer Science, 2012, 103; 614-619. doi: 10.1111/j.1349-7006.2011.02196.xAoki K, Komatsu N, Goto A, Matsuda M
Visualization of Protein Kinase Activities in Living Cells
Protein Kinase Technologies, 2012, 3-12. doi: 10.1007/978-1-61779-824-5_1
2011
Komatsu N, Aoki K (Corresponding Author) , Yamada M, Yukinaga H, Fujita Y, Kamioka Y, Matsuda M
Development of an optimized backbone of FRET biosensors for kinases and GTPases
Molecular Biology of the Cell, 2011, 22; 4647-4656. doi: 10.1091/mbc.e11-01-0072Aoki K (Corresponding Author), Yamada M, Kunida K, Yasuda S, Matsuda M
Processive phosphorylation of ERK MAP kinase in mammalian cells
Proc Natl. Acad. Sci. U.S.A., 2011, 108; 12675-12680. doi: 10.1073/pnas.1104030108Parrini MC, Sadou-Dubourgnoux A, Aoki K, Kunida K, Biondini M, Hatzoglou A, Poullet P, Formstecher E, Yeaman C, Matsuda M, Rossé C, Camonis J
SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility
Molecular Cell, 2011, 42; 650-661. doi: 10.1016/j.molcel.2011.03.032Kiyokawa E, Aoki K, Nakamura T, Matsuda M
Spatiotemporal regulation of small GTPases as revealed by probes based on the principle of Forster Resonance Energy Transfer (FRET): Implications for signaling and pharmacology
Annual Review of Pharmacology and Toxicology, 2011, 51; 337-358. doi: 10.1146/annurev-pharmtox-010510-100234
2010
Mitsushima M, Aoki K, Ebisuya M, Matsumura S, Yamamoto T, Matsuda M, Toyoshima F, Nishida E
Revolving movement of a dynamic cluster of actin filaments during mitosis
Journal of Cell Biology, 2010, 191; 453-462. doi: 10.1083/jcb.201007136Kamioka Y, Yasuda S, Fujita Y, Aoki K, Matsuda M
Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK
Journal of Biological Chemistry, 2010, 285; 33540-33548. doi: 10.1074/jbc.M110.135517Yoshiki S, Matsunaga-Udagawa R, Aoki K, Kamioka Y, Kiyokawa E, Matsuda M
Ras and Calcium Signaling Pathways Converge at Raf1 via the Shoc2 Scaffold Protein
Molecular Biology of the Cell, 2010, 21; 1088-1096. doi: 10.1091/mbc.e09-06-0455Matsunaga-Udagawa R, Fujita Y, Yoshiki S, Terai K, Kamioka Y, Kiyokawa E, Yugi K, Aoki K (Corresponding Author), Matsuda M
The scaffold protein Shoc2/SUR-8accelerates the interaction of Ras and Raf
Journal of Biological Chemistry, 2010, 285; 7818-7826. doi: 10.1074/jbc.M109.053975
2009
Aoki K, Matsuda M
Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors
Nature Protocols, 2009, 4; 1623-1631. doi: 10.1038/nprot.2009.175
2008
Tsukada Y, Aoki K, Nakamura T, Sakumura Y, Matsuda M, Ishii S
Quantification of local morphodynamics and local GTPase activity by edge evolution tracking
PLoS Computational Biology, 2008, 4; e1000223. doi: 10.1371/journal.pcbi.1000223Nakamura T, Aoki K, Matsuda M
FRET imaging and in silico simulation: analysis of the signaling network of nerve growth factor-induced neuritogenesis
Brain Cell Biology, 2008, 36; 19-30. doi: 10.1007/s11068-008-9028-5Aoki K, Kiyokawa E, Nakamura T, Matsuda M
Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Forster resonance energy transfer
Philosophical Transactions of The Royal Society B Biological Sciences, 2008, 363; 2143-2151. doi: 10.1098/rstb.2008.2267Nishioka T, Aoki K, Hikake K, Yoshizaki H, Kiyokawa E, Matsuda M
Rapid Turnover Rate of Phosphoinositides at the Front of Migrating MDCK Cells
Molecular Biology of the Cell, 2008, 19; 4213-4223. doi: 10.1091/mbc.e08-03-0315Itoh RE, Kiyokawa E, Aoki K, Nishioka T, Akiyama T, Matsuda M
Phosphorylation and activation of the Rac1 and Cdc42 GEF Asef in A431 cells stimulated by EGF
Journal of Cell Science, 2008, 121; 2635-2642. doi: 10.1242/jcs.028647
2007
Aoki K, Nakamura T, Inoue T, Meyer T, Matsuda M
An essential role for the SHIP2-dependentnegative feedback loop in neuritogenesis of NGF-stimulated PC12 cells
Journal of Cell Biology, 2007, 177; 817-827. doi: 10.1083/jcb.200609017Hisata S, Sakisaka T, Baba T, Yamada T, Aoki K, Matsuda M, Takai Y
Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth
Journal of Cell Biology, 2007, 178; 843-860. doi: 10.1083/jcb.200610073
2006
Kawase K, Nakamura T, Takaya A, Aoki K, Namikawa K, Kiyama H, Inagaki S, Takemoto H, Saltiel AR, Matsuda M
GTP Hydrolysis of a Rho-family GTPase TC10 promotes exocytic vesicle fusion
Developmental Cell, 2006, 11; 411-421. doi: 10.1016/j.devcel.2006.07.008Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M
Dynamics of the RAS/ERK map kinase cascade as monitored by fluorescence probes
Journal of Biological Chemistry, 2006, 281; 8917-8926. doi: 10.1074/jbc.m509344200Yoshizaki H, Aoki K, Nakamura T, Matsuda M
Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes
Biochemical Society Transactions, 2006, 34; 851-854. doi: 10.1042/BST0340851
2005
Nakamura T, Aoki K, Matsuda M
Monitoring spatio-temporal regulation of Ras and Rho GTPases with GFP-based FRET probes
Methods, 2005, 37; 146-153. doi: 10.1016/j.ymeth.2005.05.021Nakamura T, Aoki K, Matsuda M
FRET imaging in nerve growth cones reveals a high level of RhoA activity within the peripheral domain
Molecular Brain Research, 2005, 139; 277-287. doi: 10.1016/j.molbrainres.2005.05.030Aoki K, Nakamura T, Fujikawa K, Matsuda M
Local PIP3 Accumulation Recruits Vav2 and Vav3 to Activate Rac1/Cdc42 and Initiate Neurite Outgrowth in Nerve Growth Factor-stimulated PC12 Cells
Molecular Biology of the Cell, 2005, 16; 2207-2017. doi: 10.1091/mbc.e04-10-0904
2004
Aoki K, Nakamura T, Matsuda M
Spatio-temporal regulation of Rac1 andCdc42 activity during nerve growth factor-induced neurite outgrowth in PC12 cells
Journal of Biological Chemistry, 2004, 279; 713-719. doi: 10.1074/jbc.M306382200