Connect evolutionary changes in a population over time to a change in an environment.
Use calculated SA:V ratios to predict which cell(s) might eliminate wastes or procure nutrients faster by diffusion.
Justify the selection of data regarding the type of molecules that a plant, animal or bacteria will take up as necessary building blocks and excrete as waste.
Represent graphically / model the exchange of molecules between an organism and its environment, and the use of these molecules to build new molecules that facilitate homeostasis, growth and reproduction.
Predict the effects of change in a component(s) of a biological system on the functionality of an organism.
Apply mathematical routines to quantities that describe interactions among living systems and their environment that result in the movement of matter and energy.
Use visual representation to analyze situations or solve problems qualitatively to illustrate how interactions among living things and with their environments result in the movement of matter and energy.
Cover a 1 x 1 cm section of the underside of a leaf with nail polish.
When completely dry, place of piece of tape over the nail polish. Gently press down on the tape.
Pull of the tape and stick to a microscope slide. Label slide with appropriate information.
View slide under a microscope at low power, then high.
Draw and label observations about what is seen.
Count the number of stomata in 3 different fields of view.
Calculate the average number of stomata.
Measure the diameter of the field of view and calculate the area.
Calculate the average number of stomate per mm2.
Calculate the surface area
trace leaf on graph paper and count squares OR
cut out traced leaf and weigh it. Then compare that to the weight of the square graph paper with a known area
Calculate the relative number of stomata.
Repeat for steps for 2 other leaves.
Create a potometer
Use 1/8" plastic tubing in 2 lengths (7" & 9") and connect them with a T-connector
Attach a 1" plastic tube to the bottom of the T-connector
Insert a graduate pipet into the 7" side. Ensure it is airtight!
Bend into "J" shape and use ring stand clamps to attach tubing in place. Open end should be 1/2 height of pipet.
Fill a 10 mL syringe with water and attach it to the end of the 1" tubing.
Add water until the water level has a bead at the top of the 9" tubing (open end).
Cut the stem of a bean plant and insert the plant at least 2cm into the tubing.
Be sure to remove any air bubbles that you might see in the potometer.
Dry the area at the base of the stem and seal with petroleum jelly.
Equilibrate for 10 min.
After 10 min, zero the potometer by depressing the plunger of the syringe until the water level is at 0.
Record the initial reading as 0 mL. Record data every 10 min for 30 min.
Calculate the average rate of whole plant transpiration.
Determine the SA using methods from part 1A.
Calculate the rate of transpiration per SA.
Identify an environmental factor to test.
Follow 1B procedure.
Be sure to re-zero your potometer before.
Use the same plant as you did in part 1B.
Record data and analyze as in part 1B.
Determine the rate of transpiration.
Make a graph showing the effect of tested environmental factor on transpiration.