Supervisor Name(s): Dr. Kanhaiya Singh and Dr. Sumit Verma

Supervisor Department and School/Hospital: Department of Surgery at Indiana University

Project Title: Epigenetic Basis of Perfusion Deficit in Diabetic Ischemic Tissue 

As part of the Indiana Center for Regenerative Medicine and Engineering (ICRME) laboratory, our team is focused on developing novel strategies to improve perfusion in diabetic ischemic tissue. This project specifically aims to enhance wound recovery in diabetic patients by examining the genetic and epigenetic factors that contribute to poor circulation and delayed healing.

 As an LHSI intern, my responsibilities include learning and conducting laboratory techniques such as DNA isolation, DNA quality assessment, gene promoter methylation studies and immunohistochemistry to better understand the underlying genetics. Specifically, I have received extensive training in completing DNA methylation assays using murine DNA samples, including DNA Bisulfite Conversion, PCR, gel electrophoresis, and sending samples for Sanger sequencing. The DNA sequences obtained are then aligned to the target genome to quantify the extent of methylation. 

The findings from this project could have significant implications for diabetic patients, particularly those with critical ischemic limb and non healing diabetic ulcers. By understanding the genetic and epigenetic factors that contribute to diabetic wound healing impairment, our research may lead to the development of new therapies and interventions that can improve the quality of life for those living with this condition.

Acknowledgement: The laboratory of Dr. Kanhaiya Singh is supported by U.S. Department of Defense grant W81XWH-22-1-0146. We would like to thank Dr. Chandan K Sen, Director, ICRME for his kind support during this internship duration.