My internship at the Indiana University School of Medicine focuses on investigating vascular reactivity and endothelial function in aortas of atherosclerosis prone ApoE-/- mice. As part of this project, I have performed isometric tension experiments using aortic rings to study how blood vessels respond to chemical and mechanical stimuli. Our goal is to better understand the mechanisms behind vascular dysfunction and contribute to developing treatments for cardiovascular disease.
Throughout my internship, I conducted isometric tension recordings, prepared experimental solutions, and analyzed data to assess vascular responses. This experience enhanced my technical laboratory skills and strengthened my understanding of the critical role endothelial function plays in maintaining vascular health.
Did you know?
Mouse aortas are commonly used in isometric tension experiments because they provide important insights into how smooth muscle responds to different kinds of stimuli. These models help researchers study mechanisms that are key to understanding and treating cardiovascular conditions.
What is 'atherosclerosis'?
This video will explain what atherosclerosis is and how it happens.
Atherosclerosis is a chronic inflammatory disease characterized by plaque buildup within arterial walls, leading to reduced blood flow and an increased risk of cardiovascular events such as heart attacks and strokes. Endothelial dysfunction is a key early contributor to this disease process. This project investigates endothelial function and vascular reactivity in the ascending and descending segments of aortas from atherosclerosis-prone ApoE-/- mice. Using isometric tension experiments on isolated aortic rings, we asses how blood vessels respond to chemical and mechanical stimuli. Ultimately, this research aims to advance knowledge of the mechanisms underlying atherosclerosis and support the development of more effective treatments.
Going into this internship site as an independent worker, I brought the ability to take the initiative and manage tasks with minimal supervision, which will allow me to efficiently complete assignments during my time here. My organizational skills, developed through coursework, class projects, and being in different student organizations, ensure that I can manage multiple tasks and deadlines effectively, keeping my work structured and on track. Additionally, my motivation drives me to consistently seek ways to improve and contribute to the team, making sure I'm not only meeting but exceeding expectations. Even though I currently hold these strengths, I still want to continue working on them as I believe they are the stepping stone to my career after my undergraduate years.
Throughout my internship, I have grown into a more independent and detail-oriented team member. Initially, I focused on learning fundamental lab techniques and familiarizing myself with research protocols. As I gained more experience, my role evolved to include conducting isometric tension experiments on mouse aortas and recording data, which has allowed me to contribute more directly to our research objectives.
My primary responsibility has been assisting with isometric tension recordings, which are essential for understanding how smooth muscle in blood vessels respons to various stimuli. By carefully executing experiments and maintaining precise data records, I have helped ensure the accuracy and reliability of our findings. These contributions, though routine at times, are fundamental to the success of the larger project, as they provide critical findings into vascular function and the role of ion channels in cardiovascular health.
Even the smallest tasks, such as preparing solutions and documenting results, play a significant role in advancing our research.
Successes
1. Early in the internship, I was trained on isometric on isometric tension experiments using mouse aortas to measure vascular response to various chemical solutions. Initially, I found the intricate protocols and the precision required to be intimidating. However, through studying procedures, observing demonstrations, and receiving hands-on guidance, I successfully conducted experiments that yielded reliable data. This process not only strengthened my technical skills and understanding of smooth muscle physiology but also boosted my confidence in performing lab work. One memorable moment was when my supervisor commended my ability to maintain accuracy across several trials, which is a skill that will serve me in future research settings.
2. During a recent experiment, I was able to finally analyze the data I've collected from the isometric tension experiments. Inputting data into Excel for further analysis allowed me to visualize trends and patterns in the responses. This experience ties back directly to the quantitative skills I have developed in my coursework, such as graphing and statistical analysis in Excel, and reinforced the importance of precision and attention to detail in both data collection and interpretation. Seeing the results of my work come together has been incredibly rewarding and has strengthened my ability to bridge lab-based research with data-driven conclusions, an essential skill for my future career in forensic science.
Challenges
1. One of the challenges has been maintaining consistency in preparing the necessary material for these experiments. Initially, I struggled with calculating the correct amounts of each chemical for each solution and making the solutions. This often led to delays and occasional errors. However, by seeking guidance from my supervisor and practicing during lab hours, I have gradually improved. I now approach the experiment with greater confidence and efficiency, a skill that I can directly apply to future lab environments or future coursework involving chemistry and biology labs.
2. Juggling my internship, coursework, and organizational commitments has been a significant challenge this semester. My packed schedule often leaves me feeling overwhelmed and stretched thin. To manage my schedule, I have been using a detailed planner to organize my tasks by priority and have scheduled focused study blocks. While this has helped me stay on track and reduce stress, I recognize that I still struggle with time management. Moving forward, I plan to refine my approach by experimenting with different time blocking techniques and exploring productivity tools to optimize my schedule. This experience has reinforced my resilience and adaptability. Both of which are critical skills as I prepare for my career.