American Lung Association
Osmosis From Elsevier
Project Summary:
My research project focuses on Pulmonary Arterial Hypertension (PAH), which occurs when the blood pressure in the lungs' arteries is too high, causing the heart to work harder. My internship investigates the role of the WWOX (WW domain-containing oxidoreductase) gene in PAH and its relationship to vascular remodeling. The work hypothesizes that maintaining WWOX expression levels is crucial for the health of lung cells. When WWOX levels are low, problems can occur in cells in the lung arteries.
The whole purpose of this research is to understand how WWOX loss contributes to PAH progression by analyzing its effects on pulmonary vascular remodeling.
This research is important for understanding the role of WWOX in vascular remodeling. Understanding this role could unveil new pathways that can be targeted for PAH treatment. This could lead to developing more effective therapeutic methods, significantly improving the quality of life for PAH patients.
Our research has made some exciting progress. We have reduced the WWOX expression in cells to evaluate potential WWOX-targeting treatments for PAH, offering new treatment avenues and raising hope for the future of PAH research.
I've been a sound team member this year. Whenever someone needs something or has a favor to ask, I get it for them to the best of my ability. I assist in experiments that I know I can help with, and finally, I'm willing to learn new material that team members explain to me. We don't work in teams much, but sometimes in two-person groups; communication during experiments is usually good. Sometimes, there is a bit of miscommunication because people conduct the same experiment differently. As I learn how to do it from multiple people, I must adapt to a new style, which can trip me up. Afterward, I asked more questions than during the experiment, which I understand is a part of learning. However, it can be frustrating at times.
My team role has changed since the beginning of my internship. At first, I shadowed and observed how everyone conducted the experiments. Now I'm doing it with them or all alone, so a sense of trust has been built to do it on my own.
My contributions to helping my supervisor with her research are minimal, as I'm only at the internship site three days a week. Still, when I help her and see her research in lab meetings, I can see some of the Western Blot images I worked on and feel a sense of accomplishment.
My efforts involved running many western blots with different proteins to see how they react to antibodies, hoping to find a pathway to help with PAH. My minimal work can allow them to focus on more critical tasks.
Mice Lung Samples
Protein Quantification
Western Blot Imaging
Cell Culture Plate
Another success I'm proud of is that I'm starting to get the hang of doing calculations for solutions in experiments. I wasn't as confident at first, as it was all new to me, but after seeing the formulas and practicing with multiple methods, I started to get the hang of it, especially when you have to do it on the spot. For example, we ran out of Transfer buffer for the Western Blots. I could find the materials I needed and create the solution without assistance. When asked to explain my process, I was told I was correct and hadn't made a mistake. I've learned to pay more attention to detail when explaining things, as many solutions and instruments look the same and share common names. In my future career, I plan to work in a similar environment, so catching on to the little details now is essential, and recognizing how easy it is to make a mistake.
One of my biggest challenges is forgetting minor things that are still important, as they might end up nullifying the experiment, whether a step or a solution. My biggest challenge is that I'm timid about asking questions. If my supervisor is busy, I will greet them and wait until they are free. However, at the same time, they may ask why I haven't said anything yet.
Another challenge is not being able to finish my experiments. There are only so many hours I'm at my internship; I would have to leave in the middle of an experiment, and the next time I come in, I'm doing something completely different, as I have to work with my supervisor. Now that I have the previous experiment and a new one in my thought process, I get confused about my steps and make mistakes. My internship team has been very patient with me regarding learning, as they understand I'm also learning school materials. They have also reassured me when I make a mistake and leave in the middle of an experiment. I take accountability when I make mistakes, but they don't hold it over me; they ask that I learn from them and adapt.