Phosphate-Hach-PhosVer3

For determination of reactive (ortho) phosphate (phosphorus) by the PhosVer® 3 ascorbic acid method. Phosphate results in a blue color which is measured with the colorimeter and an 880nm (IR) led. Recommend using a stir-plate for best results.

Reagents & Equipment

  • Colorimeter with 880 nm LED (e.g. LED Ver B board)

  • Cuvettes

  • Stir plate with stir bar

  • Small beaker

  • 1 mL pipette

  • PhosVer3 Hach Part # 21060-69 for 10 mL samples, pack of 100. $31.29. Hach Method 8048 (Ascorbic acid method).

  • Distilled water. Optional, for diluting water samples.

Colorimeter set-up

  • Wavelength: 880nm. Use a custom LED board with 880 nm LED

  • Phosphate standard data: download a data file or prepare your own

  • Before measuring blank the colorimeter against water or a distilled water sample developed with PhosVer3 reagent as described below

Method

  • Transfer 10 mL of water sample into a small beaker with stir bar.

  • Add the contents of a packet of PhosVer3 to the beaker. Start mixing fairly vigorously.

  • You should see a blue color develop between 15-30 seconds.

  • After 3 minutes, take the beaker off the stir plate and transfer some to a cuvette for measurement.