Nitrate-Hach
Reagents & Equipment
Colorimeter with 400 nm LED (part # COLOR-400)
Cuvettes (macro or semi-macro)
NitraVer 5 Nitrate Reagent for 10 mL samples, Hach cat # 21061-69
Stir plate and stir bar for mixing
1 mL pipette
Small beaker
(Optional) Distilled water for sample dilutions
Colorimeter set-up
Wavelength: 400 nm
Nitrate standard data: Mid-range works well (0 - 80 ppm NO3). Download a data file or prepare your own nitrate standard data
Before measuring blank the colorimeter against water or a distilled water sample developed with NitraVer 5 reagent as described below
Method
Transfer 10 mL of water sample to a small beaker with a stir bar and place on stir plate.
Add the contents of a packet of NitraVer5 to the beaker and start mixing.
After 1 minute, take the beaker off the stir plate. Remove the stir bar.
Wait at least 5 mins for the color to develop.
Pour some of the sample into a cuvette, place in the colorimeter and click measure. The program will return the nitrate concentration in ppm.