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The core of the experimental work took place in a mesocosms system, in which plants were acclimated and then exposed to simulated heatwaves. After the plants were evaluated at three different stages (control conditions, end of the heatwave and after 5 days recovery) using non-invasive method of “mapping” of photosynthesis activity through the whole leaf surface using the chlorophyll fluorescence imagine method.
Acclimation period
Acclimation period
Seagrass shoots were translocated into mesocosms facility and exposed to control conditions of light and temperature for three weeks.
Warming ramp
Warming ramp
After the acclimation period, a heating ramp began for 7 days, with increase in temperature of 1ºC/day until maximal temperature of 28ºC was reached.
Peak temperature
Peak temperature
After the maximal temperature was reached, seagrass shoots remained at 28ºC for 5 days and then a decreasing ramp was done for 7 days with a decrease in temperature of 1ºC/day.
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