研究業績　Publication

State of the art in vivo reactive oxygen species measurements in skeletal muscle using fluorescent proteins.

R Kano, H Shirakawa, DC Poole, D Hoshino, Y Kano 

Redox Biochemistry and Chemistry

Volume 13, September 2025, 100056 

Simultaneous assessment of blood flow changes in femoral artery and skeletal muscle microvasculature using pulsed-wave and power Doppler ultrasonography.

Izumi K, Shiozawa K, Kano Y, Katayama K, Tanaka N, Akima H. Simultaneous 

Eur J Appl Physiol. 2025 Oct 12 5(10):3021-3031. doi: 10.1007/s00421-025-05813-2. Epub 2025 May 14. 

PMID: 40369285; 

Effects of pyruvate administration on mRNA expression of inflammatory cytokines in adipose tissue and whole-body glucose metabolism in male mice.

Ando T, Takeda R, Kano R, Kusano T, Nonaka Y, Kano Y, Hoshino D.

Physiol Rep. 2025 Aug13 (15):e70362. doi: 10.14814/phy2.70362. 

PMID: 40781882

Contactless monitoring of respiratory rate variability in rats under anesthesia with a compact 24GHz microwave radar sensor.
Guanghao Sun , Masaki Kurosawa , Yoshiki Ninomiya, Kohei Baba, Nguyen Huu Son , Hoang Thi Yen , Satoshi Suzuki , Yutaka Kano  
Front Vet Sci.2025 Mar26 12:1518140. doi: 10.3389/fvets.2025.1518140. eCollection 2025.

PMID: 40206262

Deuterium-reinforced polyunsaturated fatty acids protect against muscle atrophy by STZ-induced diabetic mice.
Hiroaki Eshima , Tomoaki Ishihara , Ayaka Tabuchi , Yutaka Kano , Kenji Kurokawa , Mikhail S Shchepinov 
Free Radic Biol Med. 2025 Mar16 230:273-282. doi: 10.1016/j.freeradbiomed.2025.02.015. Epub 2025 Feb 14.

PMID: 39956472

Cooling-induced changes in intracellular hydrogen peroxide and gene expression in mouse skeletal muscle in vivo.
Kano R, Takeda R, Sotani Y, Takagi R, Tabuchi A, Shirakawa H, Poole DC, Kano Y, Hoshino D.   
Am J Physiol Regul Integr Comp Physiol. 2025 Jun 1 328(6):R758-R766. doi: 10.1152/ajpregu.00014.2025. Epub 2025 May 7.
PMID: 40332827.

Intramuscular blood flow and muscle oxygenation of the vastus lateralis response to intermittent incremental muscle contractions.  

Izumi K, Yamamori K, Katayama K, Kano Y, Tanaka N, Akima H.

Exp Physiol. 2025 Jan;110(1):106-114. doi: 10.1113/EP091948. Epub 2024 Dec 10.

PMID: 39659029; 

Intracellular Ca 2+ After Eccentric Muscle Contractions: Key Role for Ryanodine Receptors.

Tabuchi A, Poole DC, Kano Y.
Exerc Sport Sci Rev. 2025 Jan 1;53(1):23-30. doi: 10.1249/JES.0000000000000348. Epub 2024 Sep 5.

PMID: 39262047 

Tabuchi A, Kikuchi Y, Takagi R, Tanaka Y, Hoshino D, Poole DC, Kano Y. In vivo intracellular Ca2+ profiles after eccentric rat muscle contractions: addressing the mechanistic bases for repeated bout protection. J Appl Physiol (1985). 2025 Jan 1;138(1):1-12. doi: 10.1152/japplphysiol.00164.2024. Epub 2024 Nov 15. PMID: 39546386.

Osana S, Tsai CT, Suzuki N, Murayama K, Kaneko M, Hata K, Takada H, Kano Y, Nagatomi R. Inhibition of methionine aminopeptidase in C2C12 myoblasts disrupts cell integrity via increasing endoplasmic reticulum stress. Biochim Biophys Acta Mol Cell Res. 2025 Mar;1872(3):119901. doi: 10.1016/j.bbamcr.2025.119901. Epub 2025 Jan 13. PMID: 39814187.

Time-series transcriptomics reveals distinctive mRNA expression dynamics associated with gene ontology specificity and protein expression in skeletal muscle after electrical stimulation-induced resistance exercise.
Kusano T, Sotani Y, Takeda R, Hatano A, Kawata K, Kano R, Matsumoto M, Kano Y, Hoshino D.
FASEB J. 2024 Nov 30;38(22):e70153. doi: 10.1096/fj.202401420RR.

PMID: 39545720 

Effect of diabetes on microvascular morphology and permeability of rat skeletal muscle: in vivo imaging using two-photon laser scanning microscopy.
Hotta K, Shimotsu R, Behnke BJ, Masamoto K, Yagishita K, Poole DC, Kano Y.
J Appl Physiol (1985). 2024 Oct 1;137(4):963-974. doi: 10.1152/japplphysiol.00222.2024. Epub 2024 Aug 15.

PMID: 39143906

Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice.
Kano R, Kusano T, Takeda R, Shirakawa H, Poole DC, Kano Y, Hoshino D.
J Appl Physiol (1985). 2024 Sep 1;137(3):778-788. doi: 10.1152/japplphysiol.00335.2024. Epub 2024 Jul 25.

PMID: 39052772

In vivo cytosolic H2O2 changes and Ca2+ homeostasis in mouse skeletal muscle.

Kano R, Tabuchi A, Tanaka Y, Shirakawa H, Hoshino D, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2024 Jan 1;326(1):R43-R52. doi: 10.1152/ajpregu.00152.2023. Epub 2023 Oct 30.

PMID: 37899753

 In vivo heat production dynamics during a contraction-relaxation cycle in rat single skeletal muscle fibers.

Tabuchi A, Tanaka Y, Horikawa H, Tazawa T, Poole DC, Kano Y.

J Therm Biol. 2024 Jan;119:103760. doi: 10.1016/j.jtherbio.2023.103760. Epub 2023 Nov 30.

PMID: 38048655

``Medical radar signal dataset for non-contact respiration and heart rate measurement" with expanded data on laboratory rats under isoflurane anesthesia.

Sun G, Kurosawa M, Ninomiya Y, Baba K, Kano Y.

Data Brief. 2024 Jul 7;55:110722. doi: 10.1016/j.dib.2024.110722. eCollection 2024 Aug.

PMID: 39109168 

Muscle Atrophy and mRNA-miRNA Network Analysis of Vascular Endothelial Growth Factor (VEGF) in a Mouse Model of Denervation-Induced Disuse.

Oguri G, Ikegami R, Ugawa H, Katoh M, Obi S, Sakuma M, Takeda N, Kano Y, Toyoda S, Nakajima T.

Cureus. 2024 Sep 9;16(9):e68974. doi: 10.7759/cureus.68974. eCollection 2024 Sep.

PMID: 39385898 

Cooling-promoted myogenic differentiation of murine bone marrow mesenchymal stem cells through TRPM8 activation in vitro.

Takagi R, Takegaki J, Osana S, Kano Y, Konishi S, Fujita S.

Physiol Rep. 2023 Dec;11(23):e15855. doi: 10.14814/phy2.15855.

PMID: 38086691 

Cmah deficiency blunts cellular senescence in adipose tissues and improves whole-body glucose metabolism in aged mice.

Takeda R, Tabuchi A, Nonaka Y, Kano R, Sudo M, Kano Y, Hoshino D.

Geriatr Gerontol Int. 2023 Dec;23(12):958-964. doi: 10.1111/ggi.14732. Epub 2023 Nov 15.

PMID: 37968438

Cooling of male rat skeletal muscle during endurance-like contraction attenuates contraction-induced PGC-1α mRNA expression.

Hoshino D, Wada R, Mori Y, Takeda R, Nonaka Y, Kano R, Takagi R, Kano Y.

Physiol Rep. 2023 Nov;11(21):e15867. doi: 10.14814/phy2.15867.

PMID: 37962014 

The aminopeptidase LAP3 suppression accelerates myogenic differentiation via the AKT-TFE3 pathway in C2C12 myoblasts.

Osana S, Kitajima Y, Naoki S, Murayama K, Takada H, Tabuchi A, Kano Y, Nagatomi R.

J Cell Physiol. 2023 Sep;238(9):2103-2119. doi: 10.1002/jcp.31070. Epub 2023 Jul 12.

PMID: 37435895

The effects of environmental enrichment on voluntary physical activity and muscle mass gain in growing rats.

Sudo M, Kano Y, Ando S.

Front Physiol. 2023 Sep 28;14:1265871. doi: 10.3389/fphys.2023.1265871. eCollection 2023.

PMID: 37841318 

Chronic repetitive cooling and caffeine-induced intracellular Ca2+ elevation differentially impact adaptations in slow- and fast-twitch rat skeletal muscles.

Takagi R, Tabuchi A, Hayakawa K, Osana S, Yabuta H, Hoshino D, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2023 Aug 1;325(2):R172-R180. doi: 10.1152/ajpregu.00063.2023. Epub 2023 Jun 19.

PMID: 37335015

Ryanodine receptors mediate high intracellular Ca2+ and some myocyte damage following eccentric contractions in rat fast-twitch skeletal muscle.

Tabuchi A, Tanaka Y, Takagi R, Shirakawa H, Shibaguchi T, Sugiura T, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2022 Jan 1;322(1):R14-R27. doi: 10.1152/ajpregu.00166.2021. Epub 2021 Nov 10.

PMID: 34755549 

 Effect of endurance training and PGC-1α overexpression on calculated lactate production volume during exercise based on blood lactate concentration.

Takeda R, Nonaka Y, Kakinoki K, Miura S, Kano Y, Hoshino D.

Sci Rep. 2022 Jan 31;12(1):1635. doi: 10.1038/s41598-022-05593-1.

PMID: 35102189 

Little involvement of recycled-amino acids from proteasomal proteolysis in de novo protein synthesis.

Osana S, Kitajima Y, Naoki S, Takada H, Murayama K, Kano Y, Nagatomi R.

Biochem Biophys Res Commun. 2022 Dec 17;634:40-47. doi: 10.1016/j.bbrc.2022.09.113. Epub 2022 Oct 2.

PMID: 36228543 

Predominant cause of faster force recovery in females than males after intense eccentric contractions in mouse fast-twitch muscle.

Watanabe D, Ikegami R, Kano Y.

J Physiol. 2021 Sep;599(18):4337-4356. doi: 10.1113/JP281927. Epub 2021 Aug 26.

PMID: 34368970 

In vivo cooling-induced intracellular Ca2+ elevation and tension in rat skeletal muscle.

Takagi R, Tabuchi A, Poole DC, Kano Y.

Physiol Rep. 2021 Jul;9(13):e14921. doi: 10.14814/phy2.14921. PMID: 34245114 

Vascular permeability of skeletal muscle microvessels in rat arterial ligation model: in vivo analysis using two-photon laser scanning microscopy.

Shimotsu R, Hotta K, Ikegami R, Asamura T, Tabuchi A, Masamoto K, Yagishita K, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2021 Jun 1;320(6):R972-R983. doi: 10.1152/ajpregu.00135.2020. Epub 2021 May 5.

PMID: 33949210 

 Type I diabetes suppresses intracellular calcium ion increase normally evoked by heat stress in rat skeletal muscle.

Ikegami R, Eshima H, Nakajima T, Toyoda S, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2021 Apr 1;320(4):R384-R392. doi: 10.1152/ajpregu.00168.2020. Epub 2021 Jan 6.

PMID: 33407019

In vivo Ca2+ dynamics during cooling after eccentric contractions in rat skeletal muscle.

Takagi R, Tabuchi A, Asamura T, Hirayama S, Ikegami R, Tanaka Y, Hoshino D, Poole DC, Kano Y.

Am J Physiol Regul Integr Comp Physiol. 2021 Feb 1;320(2):R129-R137. doi: 10.1152/ajpregu.00253.2020. Epub 2020 Nov 18.

PMID: 33206560

August Krogh: Muscle capillary function and oxygen delivery.

Poole DC, Kano Y, Koga S, Musch TI.

Comp Biochem Physiol A Mol Integr Physiol. 2021 Mar;253:110852. doi: 10.1016/j.cbpa.2020.110852. Epub 2020 Nov 24.

PMID: 33242636 

Short-Term Calorie Restriction Maintains Plasma Insulin Concentrations along with a Reduction in Hepatic Insulin-Degrading Enzyme Levels in db/db Mice.

Nonaka Y, Takeda R, Kano Y, Hoshino D.

Nutrients. 2021 Apr 3;13(4):1190. doi: 10.3390/nu13041190

PMID: 33916828 

Ayaka Tabuchi, Jesse C. Craig, Daniel M. Hirai, Trenton D. Colburn, Yutaka Kano, David C. Poole and Timothy I. Musch. Systemic NOS inhibition reduces contracting muscle oxygenation more in intact female than male rats. Nitric Oxide. 2020 Aug 1;100-101:38-44

Kouhei Amano, Yudai Nonaka Reo Takeda Yutaka Kano Daisuke Hoshino. Effects of electrical stimulation‐induced resistance exercise training on white and brown adipose tissues and plasma meteorin‐like concentration in rats. Physiol Rep. 2019;7(19):e14266. 

Yudai Nonaka, Reo Takeda, Yutaka Kano, Daisuke Hoshino. Effects of an acute 3-h swimming exercise on insulin secretion capacity of pancreatic islets. The Journal of Physical Fitness and Sports Medicine.

Watanabe D, Hatakeyama K, Ikegami R, Eshima H, Yagishita K, Poole DC, Kano Y. Sex differences in mitochondrial Ca2+ handling in mouse fast-twitch skeletal muscle in vivo. J Appl Physiol. 2020 Feb 1;128(2):241-251. 

Kitaoka Y, Watanabe D, Nonaka Y, Yagishita K, Kano Y, Hoshino D. Effects of clenbuterol administration on mitochondrial morphology and its regulatory proteins in rat skeletal muscle. Physiol Rep. 2019;7(19):e14266.

Shutaro Kodama, Manami Ohta, Kanami Ikeda, Yutaka Kano, Yoko Miyamoto, Wolfgang Osten, Mitsuo Takeda, and Eriko Watanabe. "Three-dimensional microscopic imaging through scattering media based on in-line phase-shift digital holography. Appl Opt. 2019;58(34):G345－G350. 

Tabuchi A, Eshima H, Tanaka Y, Nogami S, Inoue N, Sudo M, Okada H, Poole DC, Kano Y. Regional differences in Ca2+ entry along the proximal-middle-distal muscle axis during eccentric contractions in rat skeletal muscle. J Appl Physiol (1985). 2019;127(3):828－837. 

Hirai DM, Craig JC, Colburn TD, Eshima H, Kano Y, Musch TI, Poole DC.Skeletal muscle interstitial Po2 kinetics during recovery from contractions. J Appl Physiol (1985). 2019;127(4):930－939.

Ryo Ikegami, Hiroaki Eshima, Takuro Mashio, Tomosada Ishiguro, Daisuke Hoshino, David Poole, and Yutaka Kano. Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions. J Appl Physiol. 2019 Mar 1;126(3):691-698. 

Hirai DM, Colburn TD, Craig JC, Hotta K, Kano Y, Musch TI, Poole DC. Skeletal muscle interstitial O2 pressures: bridging the gap between the capillary and myocyte. Microcirculation. Microcirculation. 2019;26(5):e12497.

Hiroaki Eshima, David Poole, and Yutaka Kano. Mitochondrial calcium regulation during and following contractions in skeletal muscle. Review. The Journal of Physical Fitness and Sports Medicine 

Kazuki Hotta, Bradley Behnke, Kazuto Masamoto, Rie Shimotsu, Naoya Onodera, Akihiko Yamaguchi, David Poole, and Yutaka Kano. Microvascular Permeability of Skeletal Muscle After Eccentric Contraction-Induced Muscle Injury: In Vivo Imaging Using Two-Photon Laser Scanning Microscopy. J Appl Physiol (1985). 2018 Aug 1;125(2):369-380. doi: 10.1152/japplphysiol.00046.2018. Epub 2018 May 3. 

Toshiaki Nakajima, Seiichiro Koide, Tomohiro Yasuda, Takaaki Hasegawa, Tatsuya Yamasoba, Syotaro Obi, Shigeru Toyoda, Fumitaka Nakamura, Teruo Inoue, David Poole, and Yutaka Kano. Muscle hypertrophy following blood flow-restricted low force isometric electrical stimulation in rat tibialis anterior: Role for muscle hypoxia. J Appl Physiol (1985). 2018 Jul 1;125(1):134-145. doi:10.1152/japplphysiol.00972.2017. Epub 2018 Mar 22. 

Daniel M. Hirai, Jesse C. Craig, Trenton D. Colburn, Hiroaki Eshima, Yutaka Kano, William L. Sexton, Timothy I. Musch, and David C. Poole. Skeletal muscle microvascular and interstitial PO2 from rest to contractions. J Physiol. 2018 Mar 1;596(5):869-883. doi: 10.1113/JP275170. Epub 2018 Jan 30.

Aiko Watanabe, David C. Poole and Yutaka Kano. The effects of RSR13 on microvascular PO2 kinetics and muscle contractile performance in the rat arterial ligation model of peripheral arterial disease. J Appl Physiol (1985). 2017 Oct 1;123(4):764-772. doi: 10.1152/japplphysiol.00257.2017. 

Hiroaki Eshima, Shinji Miura, Nanami Senoo, Koji Hatakeyama, David C. Poole and Yutaka Kano. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1α. Am J Physiol Regul Integr Comp Physiol. 2017 Jun 1;312(6):R1017-R1028. doi: 10.1152/ajpregu.00032.2017. Epub 2017 Apr 24. 

Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, and Takayoshi Kobayashi. Photothermal imaging of skeletal muscle mitochondria.Biomedical Optics Express Vol. 8, Issue 6, pp. 2965-2975 (2017)

Mario Wakizaka, Hiroaki Eshima, Yoshinori Tanaka, Hideki Shirakawa, David C. Poole and Yutaka Kano. In vivo Ca2+ dynamics induced by Ca2+ injection in individual rat skeletal muscle fibers. Physiol Rep. 2017 Mar;5(5). pii: e13180. doi: 10.14814/phy2.13180. 

Sudo Mizuki, Ando Soichi, and Kano Y. Repeated blood flow restriction induces muscle fiber hypertrophy. Muscle Nerve. 2017 Feb;55(2):274-276. doi: 10.1002/mus.25415. 

Yoshinori Tanaka, Tadakatsu Inagaki, DavidC Poole and Yutaka Kano. pH buffering of single rat skeletal muscle fibers in the in vivo environment. Am J Physiol Regul Integr Comp Physiol. 2016 May 15;310(10):R926-33. doi: 10.1152/ajpregu.00501.2015.

電気通信大学Research Highlightsとして紹介されています． 

Yoshinori Tanaka, David C. Poole and Yutaka Kano. pH homeostasis in contracting and recovering skeletal muscle: Integrated function of the microcirculation with the interstitium and intramyocyte milieu. Curr Top Med Chem. 2016;16(24):2656-63. Review. 

Toshiaki Nakajima, Tomohiro Yasuda, Seiichiro Koide, Tatsuya Yamasoba, Syotaro Obi, Shigeru Toyoda , Yoshiaki Sato, Teruo Inoue and Yutaka Kano. Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a rat model. Heart Vessels. 2016 Oct;31(10):1685-95. doi: 10.1007/s00380-016-0801-6.