One of the most crucial, though understudied, polar ecosystems affected by increasing air and water temperatures is landfast sea ice. As sea water freezes, salt ions are pushed out during crystallization and form dense hypersaline waterways known as brine channels. These brine channels are teeming with life, though the community structure and how it is influenced by abiotic changes are largely unknown. I wanted to look at how changes in irradiance affected community dynamics and predicted that more irradiance would result in greater abundances of photosynthetic microalgae. To conduct this research, a snow gradient with gradually increasing snow depths were maintained overlying landfast Arctic sea ice. After six weeks, cores were taken from beneath the gradient, melted, and the microalgal community identified and numerated to determine the altered irradiance affects on interstitial community dynamics. Results indicated that abiotic changes in light availability and ice insulation, both heavily dependent on overlying snow, significantly affect both interstitial community abundance and diversity. Climate warming in the Arctic is likely to cause losses in overlying snow, dramatically changing the sea ice community. How these changes in the sea ice community affect the underlying marine ecosystem is still unknown.
Longleaf pine forests rely on the disturbance of fire in order to maintain a balanced ecosystem. To understand the importance of fire, this study examined the effects of fire suppression on aquatic invertebrates in ephemeral wetlands embedded in longleaf pine forests, an important habitat that links aquatic and terrestrial environments.
Within Computational Systems, the significance of programs involving systems of differential equations cannot be overemphasized. Beginning with a particular focus on the analysis and computation of the matrix exponential, we developed our results by constructing a diverse toolbox of matrix functions, having multiple applications to complex dynamical systems.
Prior studies have indicated that the transmembrane cell surface protein Integrin Alpha-6 may be upregulated in many types of cancers and promote the migration and invasion of cancer cells. Through in-vitro experimentation I tested the varying migration rates of pancreatic cancer cells expressing different forms of Integrin Alpha-6.
The advent of carbon nanotubes (CNT’s) has prompted a wide range of research in various fields but more specifically in cancer therapy for localized and site-specific treatment. We sought to affirm the initial results of CNTs in cancer therapy by investigating the toxicology in mice injected with CNT-Ab followed by microwave hypothermia.
We measured the ammonium, nitrate, potassium, pH, dissolved oxygen and conductivity parameters of two aquaponics systems that mainly differed in the settling chamber. This was done in an attempt to find which system yielded more optimal conditions for plant and fish growth.
The differentiation and migration of cells in Drosophila egg chambers are highly influenced by pathways, which are in turn influenced by specific gene expression. By using the Gal4/UAS system to manipulate the levels of Broad, the effects of gene expression can be analyzed in the migration of border cells.
Simultaneous C-C and C-N bond formation reactions were investigated from our novel organonickel(II) N-C-N pincer complex (LNiBr). Characterization of our complex and its reaction kinetics were completed by combining LNiBr with electrophilic halogenated solvents and monitored by ³¹P NMR to determine the presence of intermediates and the reaction order.
In this study, we utilized cat fleas (Ctenocephalides felis) to investigate whether natural infections with bacteria induce reactive oxygen species (ROS)synthesis in the flea gut, and whether production of ROS provides a defense mechanism against microbial colonization in the flea gut.
Predator prey interactions are a major driver of morphological evolution. The mole skink uses decoy coloration to draw predatory attacks to less lethal parts of their bodies. We wanted to know if red or blue was the more beneficial autonomous tail color in open or closed habitat
This research aims to design peptides capable of safely mimicking and inhibiting nuclear factor-kappa B transcription factors to regulate transcription and decrease overexpression. Three NF-ÎşB peptide mimics were analyzed using isothermal titration calorimetry and fluorescence to study binding affinity to ÎşB DNA. Circular dichroism was utilized to study their structures.
A novel, synthesized tris-indolyl compound (GS02) was tested for anti-cancer properties. Experimental methods included MTS and apoptosis assays. Current results show a decrease in PC3 cell viability through apoptosis. Interestingly, GS02 was shown to be toxic within a specific concentration range which may be beneficial for a new chemotherapy agent.
It has been observed that carbon nanotubes (MWCNTs) efficiently absorb microwaves, causing hyperthermia of cells in direct contact with them with unnoticed harm to other cells. It is hypothesized that a cancer treatment can be developed based on a safe microwave heating schedule for the selective ablation of tumor cells.
Amphitropical disjunct plants are species that occur in both North and South America but not in the intermediate equatorial region. How amphitropical species dispersed across the Americas to their current distribution still remains uncertain. To explain amphitropical distributions, three hypotheses were developed to test that (1) species dispersed successively through island hopping across the tropical zone in temperate microhabitats (= stepping stones), (2) species came to their current distribution through vicariance, or (3) species dispersed by a single long distance dispersal event. Twenty five amphitropical species were studied to infer their historical distributions with species distribution models in MaxEnt. Distribution models for each amphitropical species were estimated under three different timelines: the current climate, the last glacial maximum (22,000 years ago), and the last interglacial maximum (120,000–140,000 years ago). Ecological niche models were generated in MaxEnt with 19 bioclimate variables from the WorldClim database. Across the three time slices, the vicariance hypothesis was rejected in all but one species for one of its time slice. In 13 experiments, the long distance dispersal and vicariance hypotheses were rejected in favor of the stepping stone hypothesis. In 37 experiments, the stepping stone and vicariance hypotheses were rejected in favor of long distance dispersal. There were three species that could have dispersed by shorter-distance-dispersal events via intermediate dispersal from Mexico. Although our results suggest that long distance dispersal was the most dominant mechanism of dispersal among amphitropical species, they also convey that that numerous shorter-distance-dispersal events via intermediate, favorable populations are an underappreciated mode of dispersal in amphitropical species.
In this study we evaluated the importance of acoustic signals as direct cues. We conducted trials at five Peromyscus polionotus burrows. Each contained bait and emitted a predator call. These included coyote, owl, white-noise, or silence. Our results suggest that Peromyscus polionotus may use auditory cues to assess foraging risk.
While studying in vivo toxicity of this molecule in zebrafish (Danio rerio) embryo, we observed that it has a unique biological activity that causes a sudden inactivity in embryo movement. Continued investigation revealed that this molecule blocks sodium channels in neurons causing a temporary anesthesia in Danio rerio embryo.
A study of the potential anti-cancer properties of herbal agents Neem and Nilavembu. Zebrafish embryos were treated with an herbal solution for five days; on day three of five, the zebrafish were treated with human cancer and on the fifth day, the embryos were stained and observed.
Esters are common and widely used in industry. Traditional methods of ester synthesis involve a reaction between a carboxylic acid and an alcohol in the presence of a Bronsted acid catalyst. This research project focused on carrying out the ester synthesis using the electrochemical coupling of aldehydes with alcohols.
Flame retardants (THPC) used in textile processing are discharged into surface waters. However, THPC enters aquatic environments in mixture with ammonium and hydrogen peroxide, potentially affecting zooplankton in unpredictable ways. Surprisingly, zooplankton mortality appeared to be affected only by the presence of THPC, whether alone or mixed with other chemicals.