1. Computer Skills
Programming Languages – Bash, python, Tcl/Tk
Statistical analysis – Fluent with R and familiar with SPSS and Epi-info.
Parallel Computing : Beskow (CRAY-XC40-MPI) supercomputer at PDC (KTH Royal Institute of Technology), Kebnekaise/Abisko - Umeå Sweden
Operating systems – MS-DOS , Windows, Unix and Linux
Bioinformatics - Public protein and DNA databases such as EnsEMBL, SWISS-PROT, Genbank, EMBL, Interpro, ClustalW, PDB, , TMPRED among others as well as several commercial databases; Bioinformatics algorithms such as BLAST, FASTA, gene finding algorithms (GLIMMER and GENESCAN), primer designing and data mining. Evolutionary analysis with MEGA and BIO-EDIT.
Molecular Modeling – Modeller9, molecular dynamics (NAMD and OpenMM and Amber), PMV/ADT, VMD and PyMOL.
Gene Expression analysis- GEO, ArrayExpress, R/Bioconductor, Classification (WEKA and RWEKA) and Clustering
Network analysis- Meta-databases (BioGRID, BIND and DIP) and Cytoscape tools.
Next generation sequencing data analysis
2. Molecular Biology Laboratory practices
Protein extraction, purification and over expression in bacteria (E.Coli), QPCR, PCR, DNA and RNA extraction, site-directed mutagenesis, SDS-PAGE gel electrophoresis, western blotting, Restriction digestion practices, Cloning (Gateway cloning method), cell culture and cDNA synthesis.
Current: Research Fellow| National Tsing Hua University | Taiwan
1. Master of Science in Molecular Biotechnology – August 2016 to May 2018 - University of Skövde, Skövde, Sweden.
2. Research assistant – July 2017 to May 2018 – Science for Life Laboratory, Theoretical and Computational Biophysics Lab (TCBLAB), Stockholm, Sweden - Advisor Magnus Andersosn
3. Computer Laboratory assistant (Visualisation Molecular Dynamics program) – February 2018, KTH Royal Institute of Technology, SE-100 44, Stockholm, Sweden
4. Bachelor of Science in Biotechnology – August 2011 to June 2014 – Makerere University, P.O. BOX 7062 Kampala, Uganda. www.mak.ac.ug
6. Research Assistant – June to August 2013 - National Crops Resources Research Institute (NACRRI), Department of Bio-sciences, P.O Box 7084, Kampala, Uganda, www.nacrri.go.ug
July 2017 to May 2018 – Master Thesis – Registered at University of Skövde, Sweden and project work carried out at Science for Life Laboratory, Stockholm, Sweden- Determination of molecular factors that govern the specificity and affinity of lactose permease from Escherichia coli through the application of molecular dynamics. Supervisor: Magnus Andersson PhD, KTH Royal Institute of Technology, Sweden
October 2013 to April 2014 – Bachelor Thesis – Registered at Makerere University Kampala Uganda- Functional characterization and bioinformatics analysis of Dihydroflavonal 4-reductase from Rosa Hydrida. Supervisor: Masembe Charles PhD, Makerere University, Uganda
Selected publication
Genome sequencing and application for Taiwanese macaque Macaca cyclopis Kuo-Ping Chiu*, Lutimba Stuart, Hong Sain Ooi, Yu-Tai Wang, John Yu, David Glenn Smith and Kurtis Jai-Chyi Pei. Submitted
Helical structure motifs made searchable for functional peptide design Cheng-Yu Tsai, Emmanuel Oluwatobi Salawu, Hongchun Li, Guan-Yu Lin, Ting-Yu Kuo, Liyin Voon, Adarsh Sharma, Kai-Di Hu, Yi-Yun Cheng, Sobha Sahoo, Lutimba Stuart, Chih-Wei Chen, Yuan-Yu Chang, Yu-Lin Lu, Simai Ke, Christopher Llynard D. Ortiz, Bai-Shan Fang, Chen-Chi Wu, Chung-Yu Lan, Hua-Wen Fu & Lee-Wei Yang Nature Communications volume 13, Article number: 102 (2022)
Substrate Interactions in the LacY Membrane Protein Transporter Biophysical Journal, Volume 114, Issue 3, Supplement 1, 2 February 2018, Page 240a. Lutimba Stuart 1 , Stephen H. White 2 , Ronald H. Kaback 3 , Magnus Andersson 1, KTH Royal Institute of Technology, Solna, Sweden, 2 UCI, Irvine, CA, USA, 3 UCLA, Los Angeles, CA, USA.
2. 2019 (March) A travel grant to present at the Umeå Plant Science Center (UPSC), Umeå University, Sweden.
2018 (June) A travel grant to present at the Max Planck Institute of Colloids and Interfaces, University of Potsdam, Germany.
2016-2018 Swedish Institute study scholarship award for studies in molecular biotechnology at a master’s level at the University of Skövde.